Abstract: The invention relates to a device comprising: a laser source emitting a first beam with a central wavelength ?1 lying between 1010 nm and 1050 nm, a spectral supercontinuum generator downstream of the laser source, generating a second beam with a central wavelength ?2 lying between 1670 nm and 1730 nm from a part of the first beam, an optical parametric amplification system downstream of the spectral supercontinuum generator, generating a third beam with a central wavelength ?3 lying between 2540 nm and 2690 nm from at least a part of the second beam and a part of the first beam, and a second harmonic generator downstream of the optical parametric amplification system, the second harmonic generator generating a fourth beam with a central wavelength ?4 lying between 1270 nm and 1345 nm from at least a part of the third beam.

Abstract: The invention relates to a device comprising: a laser source emitting a first beam with a central wavelength ?1 lying between 1010 nm and 1050 nm, a spectral supercontinuum generator downstream of the laser source, generating a second beam with a central wavelength ?2 lying between 1670 nm and 1730 nm from a part of the first beam, an optical parametric amplification system downstream of the spectral supercontinuum generator, generating a third beam with a central wavelength ?3 lying between 2540 nm and 2690 nm from at least a part of the second beam and a part of the first beam, and a second harmonic generator downstream of the optical parametric amplification system, the second harmonic generator generating a fourth beam with a central wavelength ?4 lying between 1270 nm and 1345 nm from at least a part of the third beam.

Abstract: A module is provided for a multi-photon imaging system for simultaneously exciting chromophores of a specimen. A first femtosecond laser source emits a first pulsed excitation beam having a repetition rate 1/T and a wavelength ?1 exciting a first chromophores, the absorbed photons originating from the first excitation beam. A second femtosecond laser source emits a second pulsed excitation beam with a wavelength ?2 exciting a second chromophores, the absorbed photons originating from the second beam; the first beam including an “excitation” part exciting the specimen and a “pumping” part acting as a pump beam for exciting the second laser source to synchronize the second laser source with the first laser source. An optical delay line superimposes spatially and temporally the second beam and the excitation part of the first beam to excite at least a third chromophore, the absorbed photons originating from the first and second excitation beams.

Abstract: An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).

Abstract: An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.

Abstract: An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).

Abstract: An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal. illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.