Abstract: Identifying objects in images is a difficult problem, particularly in cases an original image is noisy or has areas narrow in color or grayscale gradient. A technique employing a convolutional network has been identified to identify objects in such images in an automated and rapid manner. One example embodiment trains a convolutional network including multiple layers of filters. The filters are trained by learning and are arranged in successive layers and produce images having at least a same resolution as an original image. The filters are trained as a function of the original image or a desired image labeling; the image labels of objects identified in the original image are reported and may be used for segmentation. The technique can be applied to images of neural circuitry or electron microscopy, for example. The same technique can also be applied to correction of photographs or videos.

Abstract: Identifying objects in images is a difficult problem, particularly in cases an original image is noisy or has areas narrow in color or grayscale gradient. A technique employing a convolutional network has been identified to identify objects in such images in an automated and rapid manner. One example embodiment trains a convolutional network including multiple layers of filters. The filters are trained by learning and are arranged in successive layers and produce images having at least a same resolution as an original image. The filters are trained as a function of the original image or a desired image labeling; the image labels of objects identified in the original image are reported and may be used for segmentation. The technique can be applied to images of neural circuitry or electron microscopy, for example. The same technique can also be applied to correction of photographs or videos.

Abstract: A method for sensing a wave-front of specimen light scattered from an illuminated area in a specimen (10) includes the steps of focusing illumination light into the specimen (10), directing specimen light scattered in the specimen (10) to a detector device (50) having a plurality of detector elements (51) and being capable to sense light with local resolution, detecting sample light contained in the specimen light with the detector device (50), said sample light being scattered in a predetermined sample plane (11) of the specimen (10) and being selected by a time-based gating of the specimen light, locally resolved measuring phase information of the sample light, and reconstructing the wave-front of the sample light on the basis of the phase information. Furthermore, a method of microscopic imaging with adapted illumination light is described.

Abstract: The present invention relates to a method of determining voltage changes, e.g. in cell membranes, by means of a voltage-sensitive dye.

Abstract: A method for sensing a wave-front of specimen light scattered from an illuminated area in a specimen (10) includes the steps of focusing illumination light into the specimen (10), directing specimen light scattered in the specimen (10) to a detector device (50) having a plurality of detector elements (51) and being capable to sense light with local resolution, detecting sample light contained in the specimen light with the detector device (50), said sample light being scattered in a predetermined sample plane (11) of the specimen (10) and being selected by a time-based gating of the specimen light, locally resolved measuring phase information of the sample light, and reconstructing the wave-front of the sample light on the basis of the phase information. Furthermore, a method of microscopic imaging with adapted illumination light is described.

Abstract: A process for operating an optical scanning system includes making an image of a sample by scanning spots in the sample, measuring intensities of light emitted by the scanned spots, determining locations of the scanned spots, and assigning intensities to image pixels based on the measured intensities and determined locations of the scanned spots. In the making of the image, the acts of determining depend on a value of a parameter. The process also includes selecting a new value for the parameter and deciding whether the image of the sample has less double imaging if the new value of the parameter is used during the acts of determining. The process accepts the new value of the parameter in response to determining that the new value produces less of the double imaging.

Abstract: An optical scanning system includes a probe and a processor. The probe includes a mechanical oscillator responsive to AC voltage signals and an optical fiber. The optical fiber has a free end that executes an oscillatory scanning motion in response to being mechanically driven by the mechanical oscillator. The processor is configured to receive measured intensities of light emitted from spots of a sample scanned by light from the free end of the optical fiber. The processor is also configured to assign intensities to image pixels based on the measured intensities of light. The acts of assigning compensate for variations in the density of the scanned spots.

Abstract: An optical scanning system includes a probe and a processor. The probe includes a mechanical oscillator responsive to AC voltage signals and an optical fiber. The optical fiber has a free end that executes an oscillatory scanning motion in response to being mechanically driven by the mechanical oscillator. The processor is configured to receive measured intensities of light emitted from spots of a sample scanned by light from the free end of the optical fiber. The processor is also configured to assign intensities to image pixels based on the measured intensities of light. The acts of assigning compensate for variations in the density of the scanned spots.

Abstract: An imaging device for microscopic imaging of an object using an objective, which is set up for generating an object image in infinity, and a fixed tube optic, which is set up to generate an intermediate image from the object imaged, are described, the objective being situated so it is movable in relation to the tube optic in at least one reference direction, which deviates from the alignment of the optical axis of the objective, and a deflection device having at least one adjustable reflector, which directs the beam path from the objective onto the tube optic in any position of the objective in such a way that it runs perpendicularly to the tube optic and parallel to its optical axis, and an adjustment device are provided, using which the objective and the at least one reflector are movable.

Abstract: An optical scanning system includes a probe and a processor. The probe includes a mechanical oscillator responsive to AC voltage signals and an optical fiber. The optical fiber has a free end that executes an oscillatory scanning motion in response to being mechanically driven by the mechanical oscillator. The processor is configured to receive measured intensities of light emitted from spots of a sample scanned by light from the free end of the optical fiber. The processor is also configured to assign intensities to image pixels based on the measured intensities of light. The acts of assigning compensate for variations in the density of the scanned spots.

Abstract: Semiconductor devices are imaged using two-photon absorption. The method is similar to conventional optical beam induced imaging except that the light beams used have frequencies (photon energies) insufficient to excite electrons across the semiconductor bandgap. Rather the instantaneous intensity of the lower frequency light is increased, as by using a pulsed laser source, so that electron transitions occur by two-photon absorption predominately in the localized region where the beam is focused. The result is minimal absorption during passage through the substrate and maximal absorption in the component-rich active layer where the beam is focused. This enhances imaging of fine-detail semiconductor devices. Specifically, the quadratic dependence of free carrier generation on the excitation intensity both enhances the resolution and provides a three-dimensional sectioning capability.

Abstract: The present invention is an inexpensive optical beam power controller for providing fast, continuous control of a linearly polarized optical beam, such as a beam from a laser. In essence, the controller comprises a birefringent plate tiltable in relation to the beam and a polarization analyzer. Alternative double pass embodiments reduce beam displacement and the required tilt angle.

Abstract: In a time-division-multiplex system, a relatively high-rate optical signal stream comprising multiple interleaved signal sequences is applied to one end of an elongated waveguide that includes multiple photodetectors disposed along the longitudinal extent of the waveguide. Probe pulses at a relatively low rate are applied to the other end of the waveguide in a synchronized fashion to cause two-photon non-linear absorption in successive respective photodetectors as each propagating probe pulse overlaps successive different signals of each sequence. In that way, electrical output signals are provided from each photodetector at the relatively low probe-pulse rate.

Abstract: A method for forming micron-sized or smaller drops of liquid, and the use of the method in fabricating micro electro mechanical and micro mechanical devices is disclosed. A micropipette is formed having an inside diameter no larger than the size of the drops to be formed. The micropipette is connected to a system capable of developing a positive and optionally negative pressure within the micropipette. The tip of the micropipette is placed in liquid. The liquid is drawn into the micropipette via capillary action or from the negative pressure developed by the system. The micropipette is then positioned to deliver liquid to an intended location on a surface. To deliver the liquid, a positive pressure is developed within the micropipette. The positive pressure forces a micron-sized or smaller drop of liquid out of the micropipette. The method can be used to form micron-sized or smaller drops of adhesive for fixing in place various structural members that form microdevices.

Abstract: A laser scanning microscope produces molecular excitation in a target material by simultaneous absorption of two photons to thereby provide intrinsic three-dimensional resolution. Fluorophores having single photon absorption in the short (ultraviolet or visible) wavelength range are excited by a stream of strongly focused subpicosecond pulses of laser light of relatively long (red or infrared) wavelength range. The fluorophores absorb at about one half the laser wavelength to produce fluorescent images of living cells and other microscopic objects. The fluorescent emission from the fluorophores increases quadratically with the excitation intensity so that by strongly focusing the laser light, fluorescence as well as photobleaching are confined to the vicinity of the focal plane. This feature provides depth of field resolution comparable to that produced by confocal laser scanning microscopes, and in addition reduces photobleaching.

Abstract: This light waveguide consists of an optically transparent body cut at one end to a sharp tip and polished optically flat at the other end. A metallization layer on its surface is thick enough to be opaque. By pressing the waveguide against a rigid plate the metallization is plastically deformed so as to expose a tiny aperture at the tip of the body through which light can pass. By carefully controlling the deformation of the metallization the diameter of the aperture can be kept between 10 and 500 nm. The waveguide can be incorporated in a semiconductor laser of a read/write head used in an optical storage device.