Abstract: A bioreactor includes a reservoir container for holding a liquid medium, a duct providing a flowpath in a generally vertical direction upward from the reservoir container, a plurality of fiber assemblies located within the duct, a top of which is higher than a top of the plurality of fiber assemblies, and a circulation system. The upper end of the duct comprises an overflow wall surrounded by a moat, a bottom of which is lower than a top of the overflow wall. The upper end of the duct and moat contact a pocket region that is bounded by a structure that is connected to the duct and that is isolated from fluid communication with an exterior of the pocket region. The liquid medium flows over the overflow wall within the pocket region, contacts gas in the pocket region, overflows into the moat and is removed therefrom by the circulation system.

Abstract: A bioreactor includes a reservoir container for holding a liquid medium, a duct providing a flowpath in a generally vertical direction upward from the reservoir container, a plurality of fiber assemblies located within the duct, a top of which is higher than a top of the plurality of fiber assemblies, and a circulation system. The upper end of the duct comprises an overflow wall surrounded by a moat, a bottom of which is lower than a top of the overflow wall. The upper end of the duct and moat contact a pocket region that is bounded by a structure that is connected to the duct and that is isolated from fluid communication with an exterior of the pocket region. The liquid medium flows over the overflow wall within the pocket region, contacts gas in the pocket region, overflows into the moat and is removed therefrom by the circulation system.

Abstract: A bioreactor for culturing of cells is described. Screens suitable as a cell growth scaffold may comprise crossed fibers. Screens may be contained loosely in a screen holder, which in turn may be contained inside a manifold assembly. A lower manifold, screen holder and upper manifold may have identical or similar interior open cross-sections. Flow of liquid medium can occur upwardly through the array of screens, then flowing over a weir in the presence of an air pocket, and into a moat and a pump. The screen holder may have slots whose exterior-facing ligaments are rounded, and may have grooves whose interior-facing edges are rounded. These components may be located inside an incubator suitable to maintain desired environmental conditions and cleanliness.

Abstract: A bioreactor for culturing of cells is described. Screens suitable as a cell growth scaffold may comprise crossed fibers. Screens may be contained loosely in a screen holder, which in turn may be contained inside a manifold assembly. A lower manifold, screen holder and upper manifold may have identical or similar interior open cross-sections. Flow of liquid medium can occur upwardly through the array of screens, then flowing over a weir in the presence of an air pocket, and into a moat and a pump. The screen holder may have slots whose exterior-facing ligaments are rounded, and may have grooves whose interior-facing edges are rounded. These components may be located inside an incubator suitable to maintain desired environmental conditions and cleanliness.

Abstract: In an embodiment of the invention, there may be provided a bioreactor having tissue scaffolds and having culture medium perfused therethrough. There may be multiple independent culture chambers and reservoirs or sub-reservoirs. Sensors can provide for individually controlling conditions in various culture chambers, and various culture chambers can be operated differently or for different durations. It is possible to infer the number of cells or the progress toward confluence from the fluid resistance of the scaffold, based on flowrate and pressure drop. Harvesting may include any combination or sequence of; exposure to harvesting reagent; vibration; liquid flow that is steady, pulsatile or oscillating; passage of gas-liquid interface through the scaffold. Vibration and flow can be applied so as to reinforce each other.

Abstract: A bioreactor for culturing of cells is described. Screens suitable as a cell growth scaffold may comprise crossed fibers. Screens may be contained loosely in a screen holder, which in turn may be contained inside a manifold assembly. A lower manifold, screen holder and upper manifold may have identical or similar interior open cross-sections. Flow of liquid medium can occur upwardly through the array of screens, then flowing over a weir in the presence of an air pocket, and into a moat and a pump. The screen holder may have slots whose exterior-facing ligaments are rounded, and may have grooves whose interior-facing edges are rounded. These components may be located inside an incubator suitable to maintain desired environmental conditions and cleanliness.

Abstract: Direct slicing of CAD models to generate process planning instructions for solid freeform fabrication may overcome inherent disadvantages of using STL format in terms of the process accuracy, ease of file management, and incorporation of multiple materials. This paper will present the results of our development of a direct slicing algorithm for layered freeform fabrication. The direct slicing algorithm was based on a neutral, international standard (ISO 10303) STEP-formatted NURBS geometric representation and is intended to be independent of any commercial CAD software. The following aspects of the development effort will be presented: 1) Determination of optimal build direction based upon STEP-based NURBS models; 2) Adaptive subdivision of NURBS data for geometric refinement; and 3) Ray-casting slice generation into sets of raster patterns.

Abstract: A device for discharging at least one substance therefrom, comprising a container having a body member with substantially a vertical wall and a closure member, the body member is provided with an upper opening via which the substance is received and a lower opening closable by the closure member, wherein the body member and said closure member together define a cavity for containing the substance and wherein the body member and the closure member is movable relative to each other for discharging the substance.

Abstract: We formulate the network-wide traffic measurement/analysis problem as a series of set-cardinality-determination (SCD) problems. By leveraging recent advances in probabilistic distinct sample counting techniques, the set-cardinalities, and thus, the network-wide traffic measurements of interest can be computed in a distributed manner via the exchange of extremely light-weight traffic digests (TD's) amongst the network nodes, i.e. the routers. A TD for N packets only requires O(loglog N) bits of memory storage. The computation of such O(loglog N)-sized TD is also amenable for efficient hardware implementation at wire-speed of 10 Gbps and beyond. Given the small size of the TD's, it is possible to distribute nodal TD's to all routers within a domain by piggybacking them as opaque data objects inside existing control messages, such as OSPF link-state packets (LSPs) or I-BGP control messages.

Abstract: An electrical accessory has releasably engageable first and second body members. The first body member includes a receiving portion adapted to be releasably engageable with a plug member of an electric cord of an electric appliance. The second body member has a first plug member that is electrically connectable with an electricity mains supply, a second plug member adapted to be releasably engageable with the first body member and an electric cord electrically connecting the first and second plug members. A securing device secures the first body member to a surface.

Abstract: In a network including a centralized controller and a plurality of routers forming a security perimeter, a method for selectively discarding packets during a distributed denial-of-service (DDoS) attack over the network. The method includes aggregating victim destination prefix lists and attack statistics associated with incoming packets received from the plurality of routers to confirm a DDoS attack victim, and aggregating packet attribute distribution frequencies for incoming victim related packets received from the plurality of security perimeter routers. Common scorebooks are generated from the aggregated packet attribute distribution frequencies and nominal traffic profiles, and local cumulative distribution function (CDF) of the local scores derived from the plurality of security perimeter routers are aggregated.