Abstract: Disclosed are fusion proteins comprising two detection portions and a periplasmic binding protein (PBP) portion between this two detection portions which undergoes a conformational change upon binding of a compound. Also described are nucleic acid molecules comprising a nucleotide sequence encoding such a fusion protein as well as expression cassettes, vectors, transgenic plants and transgenic non-human animals comprising said nucleic acid molecules. In addition, described are methods for detecting an analyte in a sample or in a cell using said fusion protein, and optionally, a control sensor allowing a calibration of analyte detection made by said fusion protein. Also described are such control sensors and nucleic acid molecules encoding them, as well as diagnostic compositions, kits and uses of the fusion protein and the control sensor for in vitro or in vivo analyte detection and preparing a diagnostic composition.

Abstract: The invention relates to a method for producing a transgenic plant in which the capability for incorporating undesired nitrogen compounds, especially amino acids, in the storage organs is reduced compared with the wild type, comprising the steps: introducing at least one DNA sequence and/or at least one RNA sequence corresponding to the DNA sequence and/or a mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence with the coding region for an amino acid transporter or parts thereof into a plant cell, wherein the DNA and/or RNA sequence and/or mixed sequence is used in sense or antisense orientation and the expression of an endogenous amino acid transporter gene is prevented or reduced, and regenerating a plant from this plant cell, wherein the DNA and/or RNA sequence is a sequence from Beta vulgaris.

Abstract: The invention relates to a nucleic acid that encodes a plant transporter of the inner mitochondrial membrane, in particular a member of the mitochondrial carrier family (MCF), and uses of this nucleic acid. The invention furthermore relates to a fragment of the nucleic acid, a construct containing the nucleic acid or a fragment thereof, and a host cell that contains the nucleic acid, the fragment, or the construct. The present invention furthermore relates to a method for producing a transgenic plant by using the nucleic acid, a fragment thereof, or a construct containing the nucleic acid or a fragment thereof, as well as a method for modulating the transport properties of the inner mitochondrial membrane of a plant, a plant part, a plant cell and/or seeds.

Abstract: There are described DNA sequences, that contain the coding region of amino acid transporters whose, introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.

Abstract: The invention relates to a nucleic acid that encodes a plant transporter of the inner mitochondrial membrane, in particular a member of the mitochondrial carrier family (MCF), and uses of this nucleic acid. The invention furthermore relates to a fragment of the nucleic acid, a construct containing the nucleic acid or a fragment thereof, and a host cell that contains the nucleic acid, the fragment, or the construct. The present invention furthermore relates to a method for producing a transgenic plant by using the nucleic acid, a fragment thereof, or a construct containing the nucleic acid or a fragment thereof, as well as a method for modulating the transport properties of the inner mitochondrial membrane of a plant, a plant part, a plant cell and/or seeds.

Abstract: There are described DNA sequences, that contain the coding region of ammonium transporters, which after introduction in suitable vectors are introduced into the plant genome and lead to the formation of mRNA which makes possible either the formation of new ammonium transporters, or prevents the formation of endogenous ammonium transporters in transgenic plants. There are further described plasmids, bacteria, yeast strains, plant cells and transgenic plants, which contain the sequences of ammonium transporters as a constituent of a recombinant DNA, and also a process for the identification and isolation of DNA sequences, coding for ammonium transporters.

Abstract: There are described DNA sequences from Arabidopsis thaliana, that contain the coding region of ammonium transporters.

Abstract: The invention relates to nucleic acid molecules that code a saccharide transporter, in particular a saccharose transporter, vectors and host cells that contain said nucleic acid molecules, as well as plant cells and plants transformed by the described nucleic acid molecules and vectors. The invention also relates to processes for modifying the transport of saccharide, in particular saccharose, in plants.

Abstract: There are described DNA sequences that contain the coding region of amino acid transporters whose introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.

Abstract: The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.

Abstract: There are described DNA sequences, that contain the coding region of ammonium transporters, which after introduction in suitable vectors are introduced into the plant genome and lead to the formation of mRNA which makes possible either the formation of new ammonium transporters, or prevents the formation of endogenous ammonium transporters in transgenic plants. There are further described plasmids, bacteria, yeast strains, plant cells and transgenic plants, which contain the sequences of ammonium transporters as a constituent of a recombinant DNA, and also a process for the identification and isolation of DNA sequences, coding for ammonium transporters.

Abstract: There are described DNA sequences from Arabidopsis that contain the coding region of amino acid transporters, as well as plasmids, bacteria, yeasts and plants containing these DNA sequences.

Abstract: Described are processes for producing plants whose flowering behavior is modified in comparison to wildtype plants, in particular for producing plants with early flower formation and flowering to an increased extent, and the plants resulting from that process. Further, the use of DNA molecules encoding sucrose carriers for modifying plant flowering behavior is described.

Abstract: There are described DNA sequences, that contain the coding region of an oligosaccharide transporter, whose introduction in a plant genome modifies the formation and transfer of storage materials in transgenic plants, plasmids, bacteria and plants containing these DNA sequences, a process for the preparation and transformation of yeast strains, that makes possible the identification of the DNA sequences of the plant oligosaccharide transporter of the invention, as well as the use of DNA sequences of the invention.

Abstract: A method for identifying substances with a potential herbicidal and growth-regulating action by means of plant transporter proteins (transporters), the use thereof and substances identifiable by the method and having a herbicidal and growth-regulating action are described.

Abstract: There are described 5' transcriptional regulatory regions of plant genes which ensure sink organ specific expression of cis-linked DNA sequences as well as recombinant double stranded DNA molecules comprising these 5' regulatory regions. Furthermore the present invention relates to a method for producing transgenic plants which show a sink tissue specific expression of a desired DNA sequence.

Abstract: There are described DNA sequences that contain the coding region of amino acid transporters whose introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.

Abstract: There are described DNA sequences, that contain the coding region of an oligosaccharide transporter, whose introduction in a plant genome modifies the formation and transfer of storage materials in transgenic plants, plasmids, bacteria and plants containing these DNA sequences, a process for the preparation and transformation of yeast strains, that makes possible the identification of the DNA sequences of the plant oligosaccharide transporter of the invention, as well as the use of DNA sequences of the invention.

Abstract: There is described a new DNA sequence of an expression cassette on which the potato tuber specific regulatory regions are localised as well as the transfer of this DNA sequence into the plant genome using agrobacteria as transfer micro-organisms. The DNA sequence contains a patatin gene with a patatin gene promoter. The transfer DNA sequence acts both for regulating endogenous as well as for preparation of heterologous products in crops.