Patents by Inventor Wolfgang Kroutil

Wolfgang Kroutil has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190127769
    Abstract: There is provided a method of producing at least one unsaturated amino acid from at least one amino acid comprising at least two carbonyl groups, the method comprising (a) contacting a recombinant microbial cell with a medium comprising the amino acid comprising the carbonyl groups, wherein the cell is genetically modified to comprise—at least a first genetic mutation that increases the expression relative to the wild type cell of an enzyme (E) selected from the CYP152 10 peroxygenase family, and—at least a second genetic mutation that increases the expression relative to the wild type cell of at least one NAD(P)+ oxidoreductase (E2) and the corresponding mediator protein.
    Type: Application
    Filed: May 3, 2017
    Publication date: May 2, 2019
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Thomas HAAS, Anja HECKER, Thomas BÜLTER, Wolfgang KROUTIL, Kurt FABER
  • Publication number: 20190070268
    Abstract: Disclosed are pharmaceutical compositions comprising a drug with a laccase-reactive functional group and a laccase (EC.10.3.2) and methods for manufacturing such compositions.
    Type: Application
    Filed: February 28, 2017
    Publication date: March 7, 2019
    Applicant: G. L. PHARMA GMBH
    Inventors: Georg GUEBITZ, Katrin GREIMEL, Martin BRANDAUER, Daniela HUBER, Klaus BLEYMAIER, Wolfgang KROUTIL, Doris LECHNER, Christof WACHTER, Harald WAGNER, Heimo WINKLER
  • Publication number: 20190060470
    Abstract: Disclosed are pharmaceutical compositions comprising oxycodone and an oxycodone-processing enzyme, wherein oxycodone is contained in the pharmaceutical composition in a storage stable, enzyme-reactive state and under conditions wherein no enzymatic activity acts on oxycodone.
    Type: Application
    Filed: February 28, 2017
    Publication date: February 28, 2019
    Applicant: G.L. PHARMA GMBH
    Inventors: Georg GUBITZ, Katrin GREIMEL, Martin BRANDAUER, Daniela HUBER, Klaus BLEYMAIER, Wolfgang KROUTIL, Doris LECHNER, Christof WACHTER, Harald WAGNER, Heimo WINKLER
  • Publication number: 20190060471
    Abstract: Disclosed is an abuse-deterrent pharmaceutical composition comprising a drug with an enzyme-reactive functional group, wherein the drug has an abuse potential, and an enzyme capable of reacting with the enzyme-reactive functional group (a drug-processing enzyme), wherein the drug with the enzyme-reactive functional group is contained in the pharmaceutical composition in a storage stable, enzyme-reactive state and under conditions wherein no enzymatic activity acts on the drug.
    Type: Application
    Filed: February 28, 2017
    Publication date: February 28, 2019
    Applicant: G.L. PHARMA GMBH
    Inventors: Georg GUEBITZ, Katrin GREIMEL, Martin BRANDAUER, Daniela HUBER, Klaus BLEYMAIER, Wolfgang KROUTIL, Doris LECHNER, Christof WACHTER, Harald WAGNER, Heimo WINKLER
  • Publication number: 20180057845
    Abstract: A biocatalytic method is provided for preparing p-vinyl phenols by a three-step, one-pot reaction according to the following reaction scheme: wherein the three steps include: (a) optionally substituted phenol (1) is bound to pyruvic acid (BTS) to form optionally substituted tyrosine (2) by the catalytic action of a tyrosine phenol-lyase (TPL) and in the presence of ammonium ions, (b) ammonia is eliminated from tyrosine (2) by the catalytic action of a tyrosine ammonia-lyase (TAL) or a phenyl ammonia-lyase (PAL) to produce optionally substituted p-coumaric acid (3), and (c) p-coumaric acid (3) is subjected to a decarboxylation reaction by the catalytic action of a phenolic acid decarboxylase (PAD), to produce the desired, optionally substituted p-vinyl phenol (4); and (d) wherein the generated CO2 is removed from the reaction system to shift the chemical equilibrium of all three reaction steps (a), (b) and (c) towards the product side.
    Type: Application
    Filed: March 3, 2016
    Publication date: March 1, 2018
    Inventors: Wolfgang KROUTIL, Eduardo BUSTO, Robert SIMON
  • Patent number: 9765370
    Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: September 19, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
  • Patent number: 9580732
    Abstract: A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: February 28, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Markus Pötter, Thomas Haas, Jan Christoph Pfeffer, Arne Skerra, Wolfgang Kroutil, Alexandra Lerchner, Johann H. Sattler, Steffen Schaffer, Katharina Christin Tauber
  • Publication number: 20160326555
    Abstract: The present invention relates to a method comprising the method steps A) Providing at least one compound of the general formula I) where X=divalent organic radical comprising 1 to 19 carbon atoms B) Contacting the compound of the general formula I) with an enzyme E1 selected from the group esterases, lipases and lactonases, characterized in that method step B) is carried out in the presence of at least one aliphatic alcohol comprising 1 to 6 carbon atoms.
    Type: Application
    Filed: December 18, 2014
    Publication date: November 10, 2016
    Applicant: EVONIK DEGUSSA GmbH
    Inventors: Philip ENGEL, Thomas HAAS, Wolfgang KROUTIL, Johann H. SATTLER
  • Patent number: 9273291
    Abstract: The invention relates to an enzyme that comprises or includes a sequence according to SEQ. ID No. 1 or SEQ. ID No. 2, to a method for the production thereof, and to the use thereof as a catalyst in the oxidative cleavage of vinyl aromatics.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: March 1, 2016
    Assignee: Universitat Graz
    Inventors: Aashrita Rajagopalan, Wolfgang Kroutil, Markus Schober
  • Publication number: 20150111254
    Abstract: The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell.
    Type: Application
    Filed: March 25, 2013
    Publication date: April 23, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
  • Publication number: 20150064756
    Abstract: The invention relates to an enzyme that comprises or includes a sequence according to SEQ. ID No. 1 or SEQ. ID No. 2, to a method for the production thereof, and to the use thereof as a catalyst in the oxidative cleavage of vinyl aromatics.
    Type: Application
    Filed: August 9, 2012
    Publication date: March 5, 2015
    Applicant: UNIVERSITAT GRAZ
    Inventors: Aashrita Rajagopalan, Wolfgang Kroutil, Markus Schober
  • Publication number: 20140308717
    Abstract: The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.
    Type: Application
    Filed: July 27, 2012
    Publication date: October 16, 2014
    Applicant: Evonik Degussa GmbH
    Inventors: Thomas Haas, Markus Poetter, Jan Christoph Pfeffer, Wolfgang Kroutil, Arne Skerra, Alexandra Lerchner, Katharina Christin Tauber, Johann H. Sattler, Steffen Schaffer
  • Publication number: 20140242646
    Abstract: The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, wherein the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme, to a whole cell catalyst for carrying out the method, and to the use of such a whole cell catalyst for oxidizing a primary alcohol.
    Type: Application
    Filed: July 17, 2012
    Publication date: August 28, 2014
    Applicant: EVONIK DEGUSSA GmbH
    Inventors: Markus Pötter, Thomas Haas, Jan Christoph Pfeffer, Arne Skerra, Wolfgang Kroutil, Alexandra Lerchner, Johann H. Sattler, Steffen Schaffer, Katharina Christin Tauber
  • Patent number: 8153408
    Abstract: A process for the racemization of an optically active alpha-hydroxyketone by incubating said alpha-hydroxyketone in the presence of an acetoin racemase of Lactobacillus.
    Type: Grant
    Filed: January 31, 2007
    Date of Patent: April 10, 2012
    Assignee: BASF SE
    Inventors: Bernhard Hauer, Rainer Stürmer, Bettina M. Nestl, Wolfgang Kroutil, Kurt Faber
  • Publication number: 20110070630
    Abstract: The invention relates to a process for enzymatic deracemization of enantiomer mixtures of secondary alcohols by a combination of oxidation and reduction reactions by means of stereoselective alcohol dehydrogenases and the cofactors thereof, wherein one enantiomer of an optically active secondary alcohol is in a formal sense selectively oxidized to the corresponding ketone, which is subsequently reduced selectively to the optical antipode, while the reduced form of the cofactor is provided for the reduction reaction by means of an additional enzyme, characterized in that two alcohol dehydrogenases with opposite stereoselectivity and different cofactor selectivity and the two corresponding, different cofactors are used for the oxidation and reduction reactions, and the oxidized and reduced cofactors are interconverted in a parallel enzymatic reaction with the additional enzyme, the direction of the deracemization toward one of the two enantiomers being controllable by the selection of the two alcohol dehydrogen
    Type: Application
    Filed: March 26, 2009
    Publication date: March 24, 2011
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Christian Gruber, Wolfgang Kroutil, Constance Voss, Jan Pfeffer, Uwe Dingerdissen
  • Patent number: 7795004
    Abstract: A method for racemizing optically active secondary alcohols by incubating these alcohols with at least one alcohol dehydrogenase of the E.C. 1.1.1. class.
    Type: Grant
    Filed: April 19, 2007
    Date of Patent: September 14, 2010
    Assignee: BASF SE
    Inventors: Rainer Stürmer, Wolfgang Kroutil, Kurt Faber, Christian Gruber
  • Publication number: 20100184174
    Abstract: The invention relates to a method for the oxidative cleavage of vinyl aromatics of the formula (1) characterized in that (a) compound(s) of the formula (1) is/are oxidized to aldehydes and ketones of the formulas (2) and (3), respectively, in the presence of molecular oxygen using at least one enzyme selected from peroxidases and laccases as a catalyst, according to the following general reaction scheme: wherein n is an integer of 0 to 5; the R1 are selected from saturated or unsaturated hydrocarbon groups with 1 to 10 carbon atoms, wherein carbon atoms are optionally substituted by heteroatoms and are optionally further substituted, amino, C1-6 alkylamino and C1-6 dialkylamino groups, halogens, hydroxy and cyano, wherein two of the substituents R1 may be linked to form a ring; R2 and R3 are each independently hydrogen or one of the options for R1, wherein R2 and/or R3 may be linked with R1 to form a ring, in which case R2 and R3 may each represent a chemical bond.
    Type: Application
    Filed: July 9, 2008
    Publication date: July 22, 2010
    Applicant: Universitat Graz
    Inventors: Wolfgang Kroutil, Miguel Lara
  • Publication number: 20090221046
    Abstract: A process for the racemisation of an optically active alpha-hydroxyketone by incubating said alpha-hydroxyketone in the presence of an acetoin racemase of Lactobacillus.
    Type: Application
    Filed: January 31, 2007
    Publication date: September 3, 2009
    Applicant: BASF SE
    Inventors: Bernhard Hauer, Rainer Stürmer, Bettina M. Nestl, Wolfgang Kroutil, Kurt Faber
  • Patent number: 7569375
    Abstract: The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.
    Type: Grant
    Filed: September 18, 2003
    Date of Patent: August 4, 2009
    Assignee: Codexis, Inc.
    Inventors: Wolfgang Stampfer, Birgit Kosjek, Wolfgang Kroutil, Kurt Faber, Jürgen Eck, Frank Niehaus
  • Publication number: 20090098623
    Abstract: A method for racemizing optically active secondary alcohols by incubating these alcohols with at least one alcohol dehydrogenase of the E.C. 1.1.1. class.
    Type: Application
    Filed: April 19, 2007
    Publication date: April 16, 2009
    Applicant: BASF SE
    Inventors: Rainer Sturmer, Wolfgang Kroutil, Kurt Faber, Christian Gruber