Patents by Inventor Wolfgang Kuhne

Wolfgang Kuhne has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10723762
    Abstract: For the removal of high molecular weight compounds from recombinantly produced polypeptides generally chromatographic methods are employed. It has been found that underivatized controlled pore glass (uCPG) selectively binds high molecular weight compounds present in a solution. The purified polypeptide can be recovered e.g. from the flow through of a chromatography column containing uCPG as chromatography material. It has been found that this effect is pronounced at a pH value of about 4 to 6 in buffered solutions. With approximately 100 m2 to 150 m2 uCPG surface per g of polypeptide almost 80% to 95% of the high molecular weight compounds are removed with a yield of 80% to 90% of polypeptide.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: July 28, 2020
    Assignee: Hoffman-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Publication number: 20190218305
    Abstract: A general method is provided for the production of purified antibodies by separation of an antibody molecule from an antibody variant by chromatographic methods, e.g. to enhance therapeutic efficacy, by for example choosing a specific harvesting time point and/or a specific purification scheme.
    Type: Application
    Filed: July 26, 2018
    Publication date: July 18, 2019
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Josef Burg, Bernhard Hilger, Thorsten Kaiser, Wolfgang Kuhne, Lars Stiens, Claus Wallerius, Frank Zettl
  • Patent number: 10273277
    Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield.
    Type: Grant
    Filed: February 19, 2016
    Date of Patent: April 30, 2019
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Roberto Falkenstein, Wolfgang Koehnlein, Wolfgang Kuhne, Hartmut Schurig
  • Publication number: 20170305964
    Abstract: For the removal of high molecular weight compounds from recombinantly produced polypeptides generally chromatographic methods are employed. It has been found that underivatized controlled pore glass (uCPG) selectively binds high molecular weight compounds present in a solution. The purified polypeptide can be recovered e.g. from the flow through of a chromatography column containing uCPG as chromatography material. It has been found that this effect is pronounced at a pH value of about 4 to 6 in buffered solutions. With approximately 100 m2 to 150 m2 uCPG surface per g of polypeptide almost 80% to 95% of the high molecular weight compounds are removed with a yield of 80% to 90% of polypeptide.
    Type: Application
    Filed: April 13, 2017
    Publication date: October 26, 2017
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Patent number: 9750564
    Abstract: A catheter for the high-frequency therapy of body lumens has a flexible shaft tube of preferably biocompatible plastic material, which is connected to a distal end piece in the region of the distal end of the catheter. A proximal part of the distal end piece, that projects into the distal part of the shaft tube, forms a damping connection to the distal end of the shaft tube, which is secured by a distal electrode which is drawn on to the external peripheral surface of the shaft tube, by the distal electrode exerting a radially inwardly acting clamping force on the damping connection.
    Type: Grant
    Filed: October 4, 2007
    Date of Patent: September 5, 2017
    Assignee: CELON AG MEDICAL INSTRUMENTS
    Inventors: Markus Fay, Wolfgang Kühne
  • Patent number: 9657054
    Abstract: For the removal of high molecular weight compounds from recombinantly produced polypeptides generally chromatographic methods are employed. It has been found that underivatized controlled pore glass (uCPG) selectively binds high molecular weight compounds present in a solution. The purified polypeptide can be recovered e.g. from the flow through of a chromatography column containing uCPG as chromatography material. It has been found that this effect is pronounced at a pH value of about 4 to 6 in buffered solutions. With approximately 100 m2 to 150 m2 uCPG surface per g of polypeptide almost 80% to 95% of the high molecular weight compounds are removed with a yield of 80% to 90% of polypeptide.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: May 23, 2017
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Publication number: 20170008941
    Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield.
    Type: Application
    Filed: February 19, 2016
    Publication date: January 12, 2017
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Roberto Falkenstein, Wolfgang Koehnlein, Wolfgang Kuhne, Hartmut Schurig
  • Patent number: 9421279
    Abstract: The invention relates to a nucleic acid preparation with a content of below 1% protein, preferably below 0.1% protein, free of ethidium bromide, phenol, cesium chloride and detergents based on octyl phenol poly(ethylene glycol ether)n and with a content of below 1 EU/mg DNA of endotoxins. Said preparation is suitable as a drug particularly in gene therapy.
    Type: Grant
    Filed: May 14, 2014
    Date of Patent: August 23, 2016
    Assignee: Roche Diagnostics GmbH
    Inventor: Wolfgang Kuhne
  • Publication number: 20160102149
    Abstract: A general method is provided for the production of purified antibodies by separation of an antibody molecule from an antibody variant by chromatographic methods, e.g. to enhance therapeutic efficacy, by for example choosing a specific harvesting time point and/or a specific purification scheme. The current invention thus reports a method for producing an antibody composition comprising an antibody molecule and a variant thereof, comprising the following steps: providing a sample comprising the antibody molecule and a variant thereof, determining the presence of the antibody molecule and/or a variant thereof and/or the ratio of the amount of the antibody molecule or variant thereof to the sum of the amounts of the antibody molecule and the variant thereof, in an aliquot of said sample, determining a subsequent harvesting time point and/or antibody purification scheme on basis of the data obtained before, thereby producing an antibody composition comprising the antibody molecule and a variant thereof.
    Type: Application
    Filed: December 18, 2015
    Publication date: April 14, 2016
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Josef Burg, Bernhard Hilger, Thorsten Kaiser, Wolfgang Kuhne, Lars Stiens, Claus Wallerius, Frank Zettl
  • Patent number: 8911437
    Abstract: The invention concerns a medical technology device for treatment of the human or animal body, comprising at least one electrical plug connector which is adapted to be connected to a complementary counterpart plug connector of a further medical technology device and which in an active position is arranged projecting upwardly at the top side of the device. To provide a medical technology device which can be used both as an individual device and also in conjunction with a second device as a device arrangement and which in that respect satisfies existing safety and aesthetic demands, it is provided in accordance with the invention that the plug connector is adapted to be movable from the active position into a passive position, wherein in the passive position the plug connector is removed from the top side of the device and the top side is closed in substantially fluid-tight relationship.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: December 16, 2014
    Assignee: Olympus Winter & Ibe GmbH
    Inventors: Andreas Hörlle, André Roggan, Wolfgang Kühne, Uwe Fischer, Timo Strauss, Stefan Schiddel, Christopher Sprenger
  • Publication number: 20140287026
    Abstract: The invention relates to a nucleic acid preparation with a content of below 1% protein, preferably below 0.1% protein, free of ethidium bromide, phenol, cesium chloride and detergents based on octyl phenol polyethylene glycol ether)n and with a content of below 1 EU/mg DNA of endotoxins. Said preparation is suitable as a drug particularly in gene therapy.
    Type: Application
    Filed: May 14, 2014
    Publication date: September 25, 2014
    Applicant: Roche Diagnostics GmbH
    Inventor: Wolfgang KUHNE
  • Patent number: 8822655
    Abstract: Herein is reported a tangential flow filtration method with a pre-filtration solute concentration adjustment in order to ensure a defined concentration of the components of the solution after tangential flow filtration.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: September 2, 2014
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Publication number: 20140163207
    Abstract: Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield.
    Type: Application
    Filed: November 26, 2013
    Publication date: June 12, 2014
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Roberto Falkenstein, Wolfgang Koehnlein, Wolfgang Kuhne, Hartmut Schurig (deceased)
  • Patent number: 8633302
    Abstract: The current invention reports a method for concentrating an immunoglobulin solution by tangential flow filtration wherein the transmembrane pressure and the cross-flow are variable.
    Type: Grant
    Filed: July 15, 2008
    Date of Patent: January 21, 2014
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Publication number: 20130315985
    Abstract: The invention relates to a nucleic acid preparation with a content of below 1% protein, preferably below 0.1% protein, free of ethidium bromide, phenol, cesium chloride and detergents based on octyl phenol poly(ethylene glycol ether)n and with a content of below 1 EU/mg DNA of endotoxins. Said preparation is suitable as a drug particularly in gene therapy.
    Type: Application
    Filed: July 10, 2013
    Publication date: November 28, 2013
    Applicant: Roche Diagnostics GmbH
    Inventor: Wolfgang KUHNE
  • Patent number: 8580933
    Abstract: The current invention reports a method for concentrating an immunoglobulin solution by tangential flow filtration wherein the transmembrane pressure and the cross-flow are variable.
    Type: Grant
    Filed: July 15, 2008
    Date of Patent: November 12, 2013
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Publication number: 20130004562
    Abstract: The invention relates to a nucleic acid preparation with a content of below 1% protein, preferably below 0.1% protein, free of ethidium bromide, phenol, cesium chloride and detergents based on octyl phenol poly(ethylene glycol ether)n and with a content of below 1 EU/mg DNA of endotoxins. Said preparation is suitable as a drug particularly in gene therapy.
    Type: Application
    Filed: July 10, 2012
    Publication date: January 3, 2013
    Applicant: ROCHE DIAGNOSTICS GMBH
    Inventor: WOLFGANG KUHNE
  • Publication number: 20120316588
    Abstract: The invention concerns a medical technology device for treatment of the human or animal body, comprising at least one electrical plug connector which is adapted to be connected to a complementary counterpart plug connector of a further medical technology device and which in an active position is arranged projecting upwardly at the top side of the device. To provide a medical technology device which can be used both as an individual device and also in conjunction with a second device as a device arrangement and which in that respect satisfies existing safety and aesthetic demands, it is provided in accordance with the invention that the plug connector is adapted to be movable from the active position into a passive position, wherein in the passive position the plug connector is removed from the top side of the device and the top side is closed in substantially fluid-tight relationship.
    Type: Application
    Filed: August 22, 2012
    Publication date: December 13, 2012
    Applicant: CELON AG MEDICAL INSTRUMENTS
    Inventors: Andreas Hörlle, André Roggan, Wolfgang Kühne, Uwe Fischer, Timo Strauss, Stefan Schiddel, Christopher Sprenger
  • Publication number: 20120219990
    Abstract: Herein is reported a tangential flow filtration method with a pre-filtration solute concentration adjustment in order to ensure a defined concentration of the components of the solution after tangential flow filtration.
    Type: Application
    Filed: August 27, 2010
    Publication date: August 30, 2012
    Inventors: Stefan Hepbildikler, Wolfgang Kuhne, Eva Rosenberg, Gerhard Winter
  • Patent number: 8251752
    Abstract: The invention relates to a high frequency surgical device for generating high frequency energy for separating and/or coagulating of biological tissue, comprising at least one energy source and at least one output socket, into which a longitudinally extending electrically conductive plug contact of an electrosurgical instrument can be inserted, and which output socket comprises a catch for engaging an opposite catch configured in the plug contact, wherein a socket contact is electrically insulated from the catch, which socket contact is electrically connected to the energy source.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: August 28, 2012
    Assignee: Celon AG Medical Instruments
    Inventor: Wolfgang Kühne