Abstract: The present invention relates to compositions comprising a therapeutic protein with enhanced stability, which comprise a piperazine- or morpholine-containing zwitterionic buffering substance, such as HEPES, in particular when used out of the common pH range. The compositions of the invention are particularly useful for topical administration The present invention further relates to the use of said compositions for treating bacterial infections, e.g. bacterial infections caused by Staphylococcus aureus such as methicillin-resistant Staphylococcus aureus (MRSA). The present invention further relates to the use of said compositions for preventing or eliminating nasal bacterial colonization or bacterial colonization of the skin.

Abstract: The use of polyalkylene oxide modified reagents is disclosed in a method for the detection of an analyte or in suitable reagent kits for such methods.

Abstract: The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space VR contains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume V0 of the matrix is available as the reaction compartment and the matrix volume VM of the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.

Abstract: The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.

Abstract: The present invention concerns a method for protein expression comprising the steps of transcribing stabilized linear short DNA in cell-free in vitro transcription/translation systems with exonuclease-containing lysates or in a cellular system containing exonucleases and subsequent translation, wherein the stability of the linear short DNA is improved by one or several of the following measures to protect the double-stranded DNA from exonucleases:

Abstract: The present invention concerns a method for producing linear DNA fragments for the in vitro expression of proteins in which a linear DNA fragment which contains control elements necessary for expression and the protein-coding gene which has complementary regions to the two ends of the linear DNA fragment at both ends, are amplified together using a primer pair which binds upstream and downstream of the expression control regions on the linear DNA fragment, characterized in that the linear DNA fragment can be produced by linearizing an expression vector. Furthermore a method is disclosed which concerns the preparation of the protein-coding gene the ends of which have regions which overlap the linear DNA fragment.

Abstract: The present invention concerns a matrix reactor for reactions in which low-molecular and high-molecular reactants are involved wherein the reactor space VR contains a matrix and comprises a reaction compartment and a supply compartment, wherein the matrix is composed of a porous material which can take up low-molecular reactants and wherein the exclusion volume V0 of the matrix is available as the reaction compartment and the matrix volume VM of the matrix is available as the supply compartment. The matrix reactor can be used for enzymatic processes, in particular for a coupled in vitro transcription/translation reaction to produce proteins.

Abstract: The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.

Abstract: The use of polyalkylene oxide modified reagents is disclosed in a method for the detection of an analyte or in suitable reagent kits for such methods.

Abstract: The invention concerns a method for the determination of an analyte in a sample liquid wherein the sample liquid is incubated with (a) a first receptor which binds to a first epitope that is only present once on the analyte and is immobilized on a particulate carrier material and (b) a second receptor which binds to a second epitope on the analyte and has at least two binding sites for the second epitope, the first epitope being different from the second epitope and the analyte is determined by means of the binding to both receptors.