Patents by Inventor Wolfgang Paffhausen
Wolfgang Paffhausen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Patent number: 8178359Abstract: A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.Type: GrantFiled: September 28, 2001Date of Patent: May 15, 2012Assignee: Bayer Healthcare AGInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 7632466Abstract: Disclosed is an ultraphobic surface structure, especially a microtitre plate and a method for the production thereof which is provided with a plurality of hydrophilic areas which are preferably distributed on the surface in a periodic manner.Type: GrantFiled: January 29, 2001Date of Patent: December 15, 2009Assignee: Qiagen GmbHInventors: Karsten Reihs, Wolfgang Paffhausen
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Patent number: 7615376Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: GrantFiled: August 27, 2008Date of Patent: November 10, 2009Assignee: Bayer Schering Pharma AktiengesellschaftInventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Publication number: 20080318270Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: ApplicationFiled: August 27, 2008Publication date: December 25, 2008Applicant: BAYER HEALTHCARE AGInventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Patent number: 7138280Abstract: In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.Type: GrantFiled: October 3, 2002Date of Patent: November 21, 2006Assignee: Bayer Healthcare AGInventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 7063952Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: GrantFiled: September 28, 2001Date of Patent: June 20, 2006Assignee: Bayer Healthcare AGInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Patent number: 6985225Abstract: The invention relates to a fluorescence-measuring system as can be employed for high-throughput screening in drug development. The arrangement for fluorescence excitation contains a two-dimensionally extended sample-receiving device and at least two illumination sources for exciting the fluorescence of the samples. The illumination sources are extended linearly and arranged in such a way that the illuminated area of the sample-receiving device is homogeneously illuminated directly or via deflecting mirrors at an opening angle of ?30°.Type: GrantFiled: May 22, 2003Date of Patent: January 10, 2006Assignee: Bayer HealthCare AktiengesellschaftInventors: Martin Bechem, Wolfgang Paffhausen
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Publication number: 20040032589Abstract: The invention relates to a fluorescence-measuring system as can be employed for high-throughput screening in drug development. The arrangement for fluorescence excitation contains a two-dimensionally extended sample-receiving device and at least two illumination sources for exciting the fluorescence of the samples. The illumination sources are extended linearly and arranged in such a way that the illuminated area of the sample-receiving device is homogeneously illuminated directly or via deflecting mirrors at an opening angle of ≦30°.Type: ApplicationFiled: May 22, 2003Publication date: February 19, 2004Inventors: Martin Bechem, Wolfgang Paffhausen
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Publication number: 20030108449Abstract: Disclosed is an ultraphobic surface structure, especially a microtitre plate and a method for the production thereof which is provided with a plurality of hydrophilic areas which are preferably distributed on the surface in a periodic manner.Type: ApplicationFiled: November 13, 2002Publication date: June 12, 2003Inventors: Karsten Reihs, Wolfgang Paffhausen
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Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Publication number: 20030092081Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: ApplicationFiled: October 3, 2002Publication date: May 15, 2003Inventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 6420183Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. The separating layer 10 must be composed such that it has a high power of reflection for the luminescent light 11.Type: GrantFiled: November 20, 1998Date of Patent: July 16, 2002Assignee: Bayer AktiengesellschaftInventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Publication number: 20020022274Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: ApplicationFiled: November 20, 1998Publication date: February 21, 2002Inventors: THOMAS KRAHN, WOLFGANG PAFFHAUSEN, ANDREAS SCHADE, MARTIN BECHEM, DELF SCHMIDT
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Publication number: 20020015969Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: ApplicationFiled: September 28, 2001Publication date: February 7, 2002Inventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
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Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays
Publication number: 20020009754Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.Type: ApplicationFiled: September 28, 2001Publication date: January 24, 2002Inventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt -
Patent number: 6191852Abstract: In the measurement system for detecting optical signals of microassays, the signal-generating test objects 5 are arranged on an investigation surface of a planar carrier 4. The planar carrier 4 is, in particular, a microtitre plate for biological objects. In principle, the measurement system comprises an optical imaging arrangement which reduces the size of the test objects 4 to be measured in such a way that all the objects are imaged completely on a two-dimensional, photosensitive image sensor 6.Type: GrantFiled: October 13, 1998Date of Patent: February 20, 2001Assignee: Bayer AktiengesellschaftInventors: Wolfgang Paffhausen, Martin Bechem
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Patent number: 5253649Abstract: An improved process for the measurement of blood circulation by means of colored microspheres is presented, which process replaces the previous counting procedure when using colored microspheres by a rapidly reproducible measurement method. This avoids the complicated and expensive use of radioactively labelled microspheres. At the same time, a coloring process for the preparation of the microspheres is presented which is distinguished by homogeneous and intensive coloration of the microspheres.Type: GrantFiled: June 7, 1991Date of Patent: October 19, 1993Assignee: Triton Technology, Inc.Inventors: Rainer Gross, Wolfgang Paffhausen, Andreas Schade, Gerd Heusch