Patents by Inventor Wolfgang Piepersberg

Wolfgang Piepersberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Processes for preparing acarviosyl transferase and for using it in the conversion of acarbose homologues into acarbose, for the preparation of acarbose homologues
    Patent number: 5989882
    Abstract: The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.
    Type: Grant
    Filed: March 14, 1997
    Date of Patent: November 23, 1999
    Assignee: Bayer Aktiengesellschaft
    Inventors: Anneliese Crueger, Hans-Georg Dellweg, Jurgen Georg Lenz, Werner Schroder, Hermann Pape, Klaus Goeke, Beate Schaper, Michael Hemker, Wolfgang Piepersberg, Jurgen Distler, Ansgar Stratmann
  • Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses
    Patent number: 5866378
    Abstract: The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:(a) substrates comprising a nucleoside monophosphate, phosphoenolpyruvate, adenosine triphosphate, and sucrose; and(b) enzymes comprising pyruvate kinase, nucleoside-monophosphate kinase, sucrose synthase and deoxythymidine-D-glucose 4,6dehydratase.
    Type: Grant
    Filed: October 7, 1996
    Date of Patent: February 2, 1999
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Ruediger Marquardt, Brigitte Hoersch, Andreas Seiffert-Stoeriko, Andreas Stein, Astrid Zervosen, Lothar Elling, Maria Regina Kula, Stefan Verseck, Juergen Distler, Wolfgang Piepersberg
  • Acarbose biosynthesis genes from actinoplanes sp., process for the isolation thereof and the use thereof
    Patent number: 5753501
    Abstract: The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has been derived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesis genes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.
    Type: Grant
    Filed: February 23, 1996
    Date of Patent: May 19, 1998
    Assignee: Bayer Aktiengesellschaft
    Inventors: Anneliese Crueger, Wolfgang Piepersberg, Jurgen Distler, Ansgar Stratmann
  • Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them and their use
    Patent number: 5710032
    Abstract: Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them, and their use.The invention concerns secondary-metabolite biosynthesis genes from actinomycetes, a method of isolating secondary-metabolite and, in particular, 6-deoxy-sugar bio-synthesis genes, from actinomycetes using the gene probes strD, strE, strL and strM gene probes from Streptomyces griseus DSM40236, and structurally related genes, as gene probes for detecting the genes snot (coding for amphotheronolide B-dTDP-D-mycosaminyl transferase), snoD (coding for dTDP-D-glucose synthase) and snoM (coding for dTDP-4-keto-6-deoxy-D-glucose isomerase), or one or more secondary-metabolite biosynthesis genes from actinomycetes. The invention also concerns the use of secondary-metabolite biosynthesis genes thus isolated.
    Type: Grant
    Filed: July 30, 1996
    Date of Patent: January 20, 1998
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Piepersberg, Barbara Brau, Petra Sichel
  • Process for the production of recombinant proteins in streptomycetes
    Patent number: 5656453
    Abstract: The invention concerns a process for the production of recombinant proteins in streptomycetes in which the expression is carried out in Streptomyces galbus DSM 40480, preferably under the control of the ermE-up promoter or an inducible promoter.
    Type: Grant
    Filed: March 31, 1995
    Date of Patent: August 12, 1997
    Assignee: Boehringer Mannheim GmbH
    Inventors: Wolfgang Piepersberg, Angela Weiss, Carola Rossler
  • Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them and their use
    Patent number: 5614619
    Abstract: Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them, and their use.The invention concerns secondary-metabolite biosynthesis genes from actinomycetes, a method of isolating secondary-metabolite and, in particular, 6-deoxy-sugar biosynthesis genes, from actinomycetes using the gene probes strD, strE, strL and strM gene probes from Streptomyces griseus DSM40236, and structurally related genes, as gene probes for detecting the genes snoT (coding for amphotheronolide B-dTDP-D-mycosaminyl transferase), snoD (coding for dTDP-D-glucose synthase) and snoM (coding for dTDP-4-keto-6-deoxy-D-glucose isomerase), or one or more secondary-metabolite biosynthesis genes from actinomycetes. The invention also concerns the use of secondary-metabolite biosynthesis genes thus isolated.
    Type: Grant
    Filed: August 25, 1994
    Date of Patent: March 25, 1997
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Piepersberg, Michael Stockmann, Kambiz M. Taleghani, J urgen Distler, Susanne Grabley, Petra Sichel, Barbara Br au