Abstract: In order to determine an analyte by a heterogeneous immunological test using a first partner of a binding pair which is immobilized on a solid phase in which a sample solution containing the analyte is incubated in the presence of the solid phase with either(1) a first conjugate consisting of (a) the second partner of the above-mentioned binding pair and (b) a first substance capable of specific immunological binding to the analyte or(2) a first conjugate consisting of (a) the second partner of the above-mentioned binding pair and (b) a first substance capable of specific immunological binding to the analyte as well as with a second labelled substance capable of specific immunological binding to the analyte,afterwards the phases are separated, in case (1) the liquid phase is replaced by a further solution containing a second labelled substance capable of binding specifically to the analyte and it is again incubated and subsequently separated, and in both cases the label on the solid phase or in the liquid pha

Abstract: The present invention provides a device for carrying out a heterogeneous reaction for the determination of a component of a sample liquid on a first capillary action carrier present in a measurement chamber, to which carrier adheres at least one non-soluble reagent, wherein at least one soluble reagent is present on a second capillary action carrier in a first pre-chamber, which carrier is in capillary contact with the capillary action carrier in the measurement chamber, which first pre-chamber is connected via a first capillary with a first application chamber to be filled from outside through a filling opening and the measurement chamber is also connected with a waste chamber by a capillary arrangement through which can only flow a liquid under a predetermined gravitational force into the measurement chamber.