Abstract: A microfluidic system for analyzing nucleic acid, the microfluidic system including a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlet

Abstract: A reagent supply device includes a plurality of chambers which are separated from each other such that different reagents may be injected, the plurality of chambers having bottom surfaces made of a membrane, the membrane comprising a destruction pattern which is formed at a position corresponding to each of the plurality of chambers and breachable by an external impact to discharge the injected reagent from the chamber.

Abstract: A method for amplifying a nucleic acid amplification in the presence of an ionic liquid that suppresses an inhibitor of nucleic acid amplification, particularly when in a biological material, and a composition useful for performing the method.

Abstract: A microfluidic device comprising a first substrate including a micro-flow path and a valve seat protruding into the micro-flow path, a second substrate coupled to the first substrate and including a cavity aligned with the valve seat, and a valve gate film provided between the first substrate and the second substrate, such that the valve gate film opens and closes the micro-flow path with air pressure applied to the cavity, wherein a surface of the valve seat is separated from the valve gate film at the initial stage such that the micro-flow path is opened at the initial stage, and the valve gate film moves to be in contact with the surface of the valve seat so that the micro-flow path is closed when air pressure is applied to the cavity.

Abstract: A microvalve device comprises: a first substrate having a first surface, where at least one fluidic channel is formed into the first surface of the first substrate; and at least one valve seat is formed in the fluidic channel; a second substrate having a second surface, the second substrate comprising at least one air channel and at least one air chamber formed on the second surface of the second substrate, where the air chamber is connected to the air channel; and an elastic film interposed between the first substrate and the second substrate, where an upper portion of the valve seat is lower than the first surface of the first substrate.

Abstract: A micro-fluid supplying device having a gas bubble trapping function. The micro-fluid supplying device includes: a fluid supplier including a fluid having a biomaterial; a trap chamber in which a gas bubble is removed from the fluid supplied from the fluid supplier; and a fluid discharger which externally discharges a material supplied from the trap chamber. Material properties of a side wall and a bottom of an inside of the trap chamber are different from each other. The side wall has a better property of wetting with respect to the fluid supplied from the fluid supplier than the bottom.

Abstract: A gene analysis apparatus includes a sample preparation chip in which a polymerase chain reaction (“PCR”) sample is prepared, a PCR chip in which a PCR is performed on the PCR sample, and a package layer on which the sample preparation chip and the PCR chip are mounted. The package layer includes a channel through which a material flows from the sample preparation chip to the PCR chip. The sample preparation chip and the PCR chip are on a same side or on opposing sides of the package layer.

Abstract: A gene analysis device including an illumination optical system radiating exciting light onto a sample solution; a microfluidic device for forming a bio-reaction space of the sample solution and including at least one micro-chamber in which a reflection pattern is formed; and a detection optical system for detecting a fluorescence signal generated by a bio-reaction in the micro-chamber and comprising a photodetector.

Abstract: A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.

Abstract: A microfluidic device including at least one microvalve which controls a flow of a fluid, where the at least one microvalve includes: a pneumatic layer, a fluidic layer disposed opposite to the pneumatic layer; an elastomer membrane disposed between the pneumatic layer and the fluidic layer; and an asymmetric valve seat protruding from a surface of the fluidic layer toward a surface of the elastomer membrane and asymmetrically dividing a space between the fluidic layer and the elastomer membrane.

Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber

Abstract: A nucleic acid analysis system comprising a seating area configured to receive a microfluidic cartridge; a pneumatic module configured to supply a pneumatic pressure or a vacuum to the cartridge when mounted on the seating area; a thermal module configured to control temperature in a predetermined portion of the cartridge when mounted on the seating area; an optic module positioned to irradiate light onto the cartridge when mounted on the seating area, and detect light generated or reflected from a sample inside the cartridge when mounted on the seating area; a fluid sensing module that determines whether a fluid in a predetermined portion of a cartridge mounted on the seating area is in a gaseous state or a liquid state; a scanning module that moves the optic module and the fluid sensing module relative to the seating area; and a control module that controls operations of the pneumatic module, the thermal module, the optic module, the fluid sensing module, and the scanning module, and processes and analyzes

Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber.

Abstract: A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber

Abstract: A microfluidic system for analyzing nucleic acid, the microfluidic system including a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlet

Abstract: A microfluidic device including a microvalve includes a first substrate, a second substrate facing the first substrate, an elastic film between the first and second substrates, a microfluidic channel on the second substrate, a valve seat of the second substrate protruding in the microfluidic channel, and a fine structure on a surface of the elastic film, facing the valve seat and which contacts the valve seat when the microvalve is operated.

Abstract: A reagent supply device includes a plurality of chambers which are separated from each other such that different reagents may be injected, the plurality of chambers having bottom surfaces made of a membrane, the membrane comprising a destruction pattern which is formed at a position corresponding to each of the plurality of chambers and breachable by an external impact to discharge the injected reagent from the chamber.

Abstract: A method for amplifying a nucleic acid amplification in the presence of an ionic liquid that suppresses an inhibitor of nucleic acid amplification, particularly when in a biological material, and a composition useful for performing the method.

Abstract: In a method for initialization of a microfluidic device including a first substrate including a microfluidic channel disposed therein, a valve seat disposed in a microfluidic channel and protruding from the first substrate, and a second substrate disposed opposite to the first substrate and including a space formed therein and corresponding to the valve seat, and a polymer film disposed between the first and second substrates, the method includes separating the polymer film from a surface of the valve seat by applying a positive pressure into the microfluidic channel of the first substrate and applying a negative pressure into the space of the second substrate.

Abstract: A microfluidic device comprising a first substrate including a micro-flow path and a valve seat protruding into the micro-flow path, a second substrate coupled to the first substrate and including a cavity aligned with the valve seat, and a valve gate film provided between the first substrate and the second substrate, such that the valve gate film opens and closes the micro-flow path with air pressure applied to the cavity, wherein a surface of the valve seat is separated from the valve gate film at the initial stage such that the micro-flow path is opened at the initial stage, and the valve gate film moves to be in contact with the surface of the valve seat so that the micro-flow path is closed when air pressure is applied to the cavity.