Abstract: An artificial insemination device for private use which is capable of allowing unskilled persons to achieve an artificial insemination of livestock such as cattle, horses, and deer, etc. in a simple and easy manner using frozen semen of those livestock. The device includes an in-vagina insert unit inserted into the vagina of a female to be inseminated upon injecting semen into the female, and a semen injection unit inserted into the womb canal of the female through the in-vagina insert unit upon injecting semen into the female. The in-vagina insert unit includes a hollow insert body, and an insert tube slidably fitted at its rear end in a front end of the insert body in such a manner that its length protruded from the insert body is adjustable in accordance with the depth of the vagina of the female. The semen injection unit includes an elongated semen injection tube connected at a rear end thereof to an injector, and a guide/nozzle member separably coupled to a front end of the semen injection tube.