Abstract: A specimen base, a specimen sectioning device, a microscopic apparatus and a method for 3D imaging of biological specimen. The specimen base comprising a top plate (1) and a bottom plate (2) that are detachably coupled together; the top plate (1) comprises a first plane (11) and a second plane (12) opposite each other, the first plane (11) is used for fixing specimen, and the second plane (12) is coupled with a third plane (21) of the bottom plate (2); a top plate fixing part (13) is provided on the second plane (12) and a bottom plate fixing part (22) is provided at the corresponding position on the third plane (21); the top plate (1) couples with the bottom plate (2) by means of matching the top plate fixing part (13) with the bottom plate fixing part (22); a through-hole (23) is provided in the bottom plate (2).

Abstract: Provided are a reagent for clearing tissue or organ, a reagent for dehydration of tissue or organ, a transparent embedding solvent system (TESOS) for transparent embedding tissue or organ, a method for clearing tissue or organ, a method for transparent embedding tissue or organ using the TESOS, and a method for microscopic imaging.