Abstract: The invention provides in an electron microscopy grid, comprising: a perforated substrate; a support film on the perforated substrate; a mixture of different linker molecules according to Structure (I), wherein AG is an anchoring group, for anchoring the linker molecule to the solid support; BU is a binding unit, for binding to the analyte; L1 is a first linear linker section; L2 is a second linear linker section; ? is the angle between the linear linker section L1 and the linear linker section L2; AS is an angled linker section, connecting the linear linker section L1 and the linear linker section L2. The invention further provides in method of structural determination of analytes, using such EM-grids.

Abstract: The invention provides a method for regioselective functionalizing a surface of an electron microscopy grid (10), therefore: a) providing a surface (20) of an electron microscopy grid (101); b) providing a plurality of wall systems (30A, 30B) on the surface of an electron microscopy grid, thereby forming a plurality of temporary reaction chambers (40A, 40B); c) providing a modification solution (50) in each temporary reaction chambers (40A, 40B); d) modifying the parts of the surface (21A, 21B) in the temporary reaction chambers (40A, 40B) with the modification solution(s) (50, 50A, 50B); e) removing the modification solution(s) (50, 50A, 50B) from the temporary reaction chambers (40A, 40B); f) removing the plurality of wall systems (30A, 30B) from the surface (20). The invention further provides in an electron microscopy grid to be regioselective functionalised and an apparatus for regioselective functionalizing a surface of an electron microscopy grids.

Abstract: An electron microscopy grid, includes: (i) a perforated substrate, (ii) a support film on the perforated substrate, the support film having a thickness of 60 ? or less, and (iii) linkers attached on top of the support film. The linkers has at least one affinity group for immobilizing an analyte; wherein the linkers form a non-random pattern on the support film.

Abstract: The invention provides in an electron microscopy grid, comprising: - a perforated substrate; - a support film on the perforated substrate; - a mixture of different linker molecules according to Structure (I), wherein AG is an anchoring group, for anchoring the linker molecule to the solid support; BU is a binding unit, for binding to the analyte; L1 is a first linear linker section; L2 is a second linear linker section; ? is the angle between the linear linker section L1 and the linear linker section L2; AS is an angled linker section, connecting the linear linker section L1 and the linear linker section L2. The invention further provides in method of structural determination of analytes, using such EM-grids.

Abstract: An electron microscopy grid, includes: (i) a perforated substrate, (ii) a support film on the perforated substrate, the support film having a thickness of 60 ? or less, and (iii) linkers attached on top of the support film. The linkers has at least one affinity group for immobilizing an analyte; wherein the linkers form a non-random pattern on the support film.