Abstract: A toothbrush box with sterilization function includes a box body, a fixing rack, an ozone generator, a negative ion generator, an ultraviolet lamp, and an antibiotic filter that is coated with a nano-silver on a surface thereof and is wrapped on the ultraviolet lamp, wherein the fixing rack and the ultraviolet lamp are mounted on an upper surface of a bottom portion of the box body, and the ozone generator and the negative ion generator are installed in a compartment of the bottom portion. When the toothbrush box is power supplied, the ultraviolet lamp wrapped with the antibiotic filter, the ozone generator and the negative ion generator can sterilize toothbrushes placed in the box. Moreover, the negative ion generator can produce negative ions to dehumidify and deodorize the toothbrushes.