Abstract: This invention relates to a method for determining fruiting body and mycelium of Antrodia cinnamomea and Antrodia salmonea. By using ten known components in Antrodia cinnamomea including five ergostanes (antcins C and K, zhankuic acids A, B and C), four lanostanes (sulphurenic acid, dehydrosulphurenic acid, eburicoic acid, dehydroeburicoic acid), and one monophenyl (4,7-dimethoxy-5-methyl-1,3-benzodioxole) as standards, differentiation of mycelia and basidiomes of Antrodia cinnamomea was carried out in the invention. The natural basidiomes collected from wood of Cinnamomum kanehirai at natural forests and the cultural basidiomes grown on potato dextrose agar medium contained all ten tested components. However, the natural mycelia collected from wood of C. kanehirai at a natural forest and the liquid/solid cultural mycelia grown on potato dextrose broth/potato dextrose agar media contained the four lanostanes and 4,7-dimethoxy-5-methyl-1,3-benzodioxole, but not the five ergostanes.