Abstract: The present disclosure provides a tFiber protein fragment of avian egg drop syndrome virus, wherein the gene that encoded the tFiber protein fragment of avian egg drop syndrome virus has a nucleotide sequence shown in SEQ ID NO. 2 or a degenerate sequence thereof, and a vaccine composition prepared from the protein fragment. The fragment has good immunogenicity and is capable of preparing combined vaccine with various antigens, producing complete protection to the chickens and ducks.