Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups.

Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups.

Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups.

Abstract: Disclosed herein are methods of treating an edible fiber source to make an animal feed with increased digestible energy. An exemplary method includes hydrolyzing the edible fiber source with an inorganic fiber hydrolyzing agent in a twin screw mixer that shears the edible fiber to a size of between 0.5 to 25 mm. The hydrolysis in the mixer occurs at pressure of about 14 psig or higher with a temperature about 100° C. to 110° C. The inorganic hydrolysis liberates a first portion of soluble carbohydrates from the edible fiber source. The inorganically hydrolyzed material is also treated (before or after) with a fiber degrading enzyme to solubilize a second portion of carbohydrates. The dually hydrolyzed material is dried to form an animal feed or feed ingredient having a soluble and insoluble carbohydrate fraction with the amount of soluble carbohydrate being at least 45% wt/wt of the total carbohydrates obtained from the edible fiber source.

Abstract: The invention is drawn to the enzymatic decarboxylation of 2-keto-L-gulonic acid (2-KLG) to produce xylose. The invention is also drawn to a method to detect xylose in vitro or in vivo (intracellularly), which employs an L-xylose dehydrogenase.

Abstract: The present invention relates to methods for producing propylene glycol monoesters (PGMEs). A method comprises contacting a fatty acid material with a lipase to form a first composition, contacting the first composition with propylene glycol to form a second composition, and heating the second composition, wherein a PGME composition is produced. The present methods yield between about 40% to about 70% PGMEs or greater, and less than about 15% propylene glycol diester.

Abstract: Methods for cell-free production of glucosamine from starch, maltodextrin or glycogen or from fructose and a source of amino groups are disclosed. Also disclosed are cellular extracts comprising glucosamine-6-phosphate synthase activity, as well as a cellular extract comprising glucosamine-6-phosphate deaminase.

Abstract: Embodiments of the present invention are directed to a method of producing diacylglycerides comprising mixing one or more fatty acid esters of glycerol and at least one enzyme selected from a lipase and an endo-lipase and transesterifying at least a portion of the one or more fatty acid esters of glycerol to produce diacylglycerides. The enzyme may be a free enzyme or the enzyme may be immobilized. The fatty acid esters of glycerol may comprise at least one of monoacylglycerides, diacylglycerides, and triacylglycerides. Additionally, embodiments of the method of the present invention may comprise mixing at least one of a fatty acid and a fatty acid lower alcohol ester with the one or more fatty acid esters of glycerol and the enzyme. Additional embodiments may comprise mixing glycerol with the one or more fatty acid esters of glycerol and the enzyme.

Abstract: The invention is drawn to the enzymatic decarboxylation of 2-keto-L-gulonic acid (2-KLG) to produce xylose. The invention is also drawn to a method to detect xylose in vitro or in vivo (intracellularly), which employs an L-xylose dehydrogenase.

Abstract: Methods for cell-free production of glucosamine from starch, maltodextrin or glycogen or from fructose and a source of amino groups are disclosed. Also disclosed are cellular extracts comprising glucosamine-6-phosphate synthase activity, as well as a cellular extract comprising glucosamine-6-phosphate deaminase.