Abstract: Provided is a perfusion culture method for CAR-T cells. The method comprises the following steps: 1) separating peripheral blood mononuclear cells from a single blood cell of a subject, and sorting the mononuclear cells to obtain T cells; 2) carrying out activation treatment on the separated T cells by using CD3/CD28 stimulation magnetic beads; 3) infecting the activated T cells by using a lentiviral vector; 4) carrying out perfusion culture on the lentivirus-infected T cells, and harvesting CAR-T cells, wherein the composition of a serum-free culture medium, which does not contain an animal-derived component, for culturing the CAR-T cells is: AIM-V+(3-9)% ISR; and the perfusion culture comprises the following stages: the first stage: when the cell density is (0.5-1.1)×106 cells/mL, the perfusion rate is A1, and/or the second stage: when the cell density is (1.

Abstract: Methods, apparatuses, systems, assemblies, and/or the like are provided. An example shuttle car assembly may include a shuttle car body configured to support one or more objects. The shuttle car body includes a chassis, one or more wheels connected to the chassis, one or more operating components connected to the chassis, one or more toolless body attachments. The shuttle car assembly may also include a shuttle car housing configured to at least partially house the one or more operating components of the shuttle car body. The shuttle car housing may include a shell with a top surface, a front surface, and two opposed side surfaces, and one or more toolless housing attachments. The one or more toolless body attachments and the one or more toolless housing attachments may be configured to engage to operatively connect the shuttle car body and the shuttle car housing.

Abstract: A processing device (30, 710) for reducing scan traffic is provided. The processing device (30, 710) comprises one or more interfaces (32, 718) configured to transmit information to at least one register access interface (759, 761) and processing circuitry (34) configured to control the one or more interfaces. Further, the processing circuitry (34) is configured to obtain register parameters of at least one functional unit (760, 762) of a processing unit (750) and to generate an improved bulk register comprising the register parameters of the at least one functional unit.

Abstract: The present invention provides a test device for nucleic acid, including a sample treatment chamber, a sample reaction chamber and a test chamber which are disposed from top to bottom successively. The functions of sampling, nucleic acid purification, isothermal amplification and testing result reading by immunochromatography are integrated onto a device to prepare a simple and cute test device for nucleic acid; the device can achieve nucleic acid testing only by two rotations and can ensure that each sampling or reagent adding can reach the target area in a free falling way without any extra drainage facility. The present invention has lower costs, more convenient operation, high detection sensitivity, short time required in nucleic acid testing, and can be used for nucleic acid testing of various samples, such as human and animals.

Abstract: Methods and kits are provided for detecting and discriminating between immune quiescence and allograft rejections, such as T cell-mediated rejection and antibody-mediated rejection, using differential gene expression signatures alone or in combination with levels of transplant-derived cell-free nucleic acids.

Abstract: Wind baffles are provided at a side discharge vent of a cabinet containing a heating, ventilation, air conditioning, and refrigeration (HVACR) system. The wind baffles include two or more baffles joined to a support frame. The baffles have a leading edge towards ambient environment surrounding of the cabinet and a trailing edge towards an interior of the cabinet, and the leading edge has a perimeter greater than a perimeter of the trailing edge. The baffles may surround each successive baffle. The baffles may be concentric The wind baffles may be located between ambient environment surrounding of the cabinet and a fan near the side discharge vent. The fan may be a variable-speed fan.

Abstract: Apparatuses are described that provide for a mobile eye washing station. An example station includes a fluid storage cartridge that stores unused eye wash fluid and a nozzle assembly in fluid communication with the fluid storage cartridge. In operation, the nozzle assembly dispenses the unused eye wash fluid stored in the fluid storage cartridge via one or more nozzles of the nozzle assembly. The example station further includes a waste fluid collection basin that, in operation, collects eye wash fluid dispersed by the nozzle assembly. Due to contact with the fluid storage cartridge, the waste fluid collection basin compresses the fluid storage cartridge to drive fluid communication between the fluid storage cartridge and the nozzle assembly. The fluid storage cartridge is disposed vertically beneath the waste fluid collection basin.

Abstract: A face mask comprises a container coupled to a first surface of a face mask, a fragrance material disposed in the container, and an exhalation valve coupled to a second surface of the a face mask. The fragrance material comprises a fluid configured to evaporate from the fragrance material, and the container is configured to releasably engage the exhalation valve to form a closed chamber. The closed chamber is configured to enclose the fragrance material when the container is releasably engaged with the exhalation valve.

Abstract: Embodiments relate generally to a gas detector system. The gas detector system comprises a gas detector mounting flange, where the gas detector mounting flange comprises an explosion-proof cylindrical body that is configured to couple the gas detector mounting flange to a gas detector and a plurality of bolt receiving slots configured to couple the gas detector mounting flange to a mounting system by securing bolts, where each bolt receiving slot is at least three times as long as a diameter of securing bolts.

Abstract: Apparatus and associated methods relate to a body-worn unitary harness having a shoulder portion and a waist portion in tensile communication with one another, such that both the shoulder and the waist portions can be simultaneously adjusted using a single adjustment mechanism to conform to a wearer's body. In an illustrative embodiment, a length of webbing may thread through an angle-turning buckle and change direction therein. Tensile forces in the length of webbing may be directed in different directions on either side of the angle-turning buckle. A single clasp may facilitate both donning and doffing of the unitary harness. An implement connector may be coupled to a hip member and configured to connect an implement to the unitary harness. Various exemplary unitary harnesses may provide comfortable implement carrying capacity while automatically adjusting relative waist and shoulder lengths to accommodate a user's movements and positions.

Abstract: Wind baffles are provided at a side discharge vent of a cabinet containing a heating, ventilation, air conditioning, and refrigeration (HVACR) system. The wind baffles include two or more baffles joined to a support frame. The baffles have a leading edge towards ambient environment surrounding of the cabinet and a trailing edge towards an interior of the cabinet, and the leading edge has a perimeter greater than a perimeter of the trailing edge. The baffles may surround each successive baffle. The baffles may be concentric The wind baffles may be located between ambient environment surrounding of the cabinet and a fan near the side discharge vent. The fan may be a variable-speed fan.