Patents by Inventor XIAFENG LU

XIAFENG LU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nitrilase mutant, construction method therefor, and application thereof
    Patent number: 11492606
    Abstract: The present invention discloses a nitrilase mutant and its construction method and its application in the synthesis of chiral intermediate of pregabalin in the technical field of bioengineering. The present invention, respectively, takes turnip nitrilase BrNIT and arabidopsis nitrilase AtNIT as parent, using peptide fragment displacement method, displaces the sites 226-286 of BrNIT amino acid sequence and sites 225-285 of AtNIT amino acid sequence with sites 225-285 of Arabis alpina L. nitrilase AaNIT, obtain nitrilase mutants BrNIT225-285 and AtNIT225-285 of which the amino acid sequence is as shown in SEQ ID NO.1 or SEQ ID NO.3.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: November 8, 2022
    Assignee: ZHEJIANG UNIVERSITY OF TECHNOLOGY
    Inventors: Renchao Zheng, Yuguo Zheng, Qin Zhang, Zheming Wu, Xiaoling Tang, Xiafeng Lu
  • NITRILASE MUTANT, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF
    Publication number: 20210009981
    Abstract: The present invention discloses a nitrilase mutant and its construction method and its application in the synthesis of chiral intermediate of pregabalin in the technical field of bioengineering. The present invention, respectively, takes turnip nitrilase BrNIT and arabidopsis nitrilase AtNIT as parent, using peptide fragment displacement method, displaces the sites 226-286 of BrNIT amino acid sequence and sites 225-285 of AtNIT amino acid sequence with sites 225-285 of Arabis alpina L. nitrilase AaNIT, obtain nitrilase mutants BrNIT225-285 and AtNIT225-285 of which the amino acid sequence is as shown in SEQ ID NO.1 or SEQ ID NO.3.
    Type: Application
    Filed: January 31, 2019
    Publication date: January 14, 2021
    Inventors: RENCHAO ZHENG, YUGUO ZHENG, QIN ZHANG, ZHEMING WU, XIAOLING TANG, XIAFENG LU