Abstract: The present invention relates to a detection mechanism for polymerase chain reaction and a polymerase chain reaction device, wherein the detection mechanism comprises at least one excitation module group, each of the excitation module groups comprising two excitation modules for providing excitation light with two wavelengths; an excitation optical fiber, connected to the excitation modules, the excitation optical fiber transmitting the excitation light to at least one reaction tube, each of the reaction tubes receiving excitation light with two wavelengths; a receiving optical fiber, for collecting and transmitting a fluorescent signal from the reaction tube; at least one receiving module group, connected to the receiving optical fiber, each of the receiving module groups comprising two receiving modules, to respectively receive the fluorescent signal of two wavelengths from the same said reaction tube, and convert the fluorescent signal into an electrical signal for output; the detection mechanism is config

Abstract: Disclosed herein is a method of enhancing RAA isothermal amplification sensitivity using magnetic beads and applications in nucleic acid analysis and detection and medical diagnosis, belonging to the biomedical engineering field, comprising: (1) material selection of magnetic beads, (2) optimization of diameter of magnetic beads, (3) optimization of mixing time of magnetic beads, and (4) sensitivity detection method of magnetic beads for RAA isothermal detection, wherein the material of magnetic bead is steel bead, the diameter of the magnetic bead is 1.5 mm, the mixing time of the magnetic bead is 30 s, and the detection method of isothermal RAA results includes agarose gel and fluorescence detection. Under this condition, the sensitivity of magnetic beads for RAA isothermal amplification is increased by 10 times compared to that without adding magnetic beads, and increased by 100 times compared to that of other types of fluorescence detectors.

Abstract: The present invention relates to a detection mechanism for polymerase chain reaction and a polymerase chain reaction device, wherein the detection mechanism comprises at least one excitation module group, each of the excitation module groups comprising two excitation modules for providing excitation light with two wavelengths; an excitation optical fiber, connected to the excitation modules, the excitation optical fiber transmitting the excitation light to at least one reaction tube, each of the reaction tubes receiving excitation light with two wavelengths; a receiving optical fiber, for collecting and transmitting a fluorescent signal from the reaction tube; at least one receiving module group, connected to the receiving optical fiber, each of the receiving module groups comprising two receiving modules, to respectively receive the fluorescent signal of two wavelengths from the same said reaction tube, and convert the fluorescent signal into an electrical signal for output; the detection mechanism is config

Abstract: Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids.

Abstract: Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids.

Abstract: Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.

Abstract: Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.

Abstract: Disclosed are mechanically-actuated devices for transporting fluids within a microfluidic circuit and performing diagnostic operations on a sample. Also disclosed are related methods for performing sample analysis effected by the motion of an actuator proximate to a microfluidic system.

Abstract: Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.

Abstract: Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.