Abstract: The present disclosure provides method and apparatus for generating a response in a human-machine conversation. A first sound input may be received in the conversation. A first audio attribute may be extracted from the first sound input, wherein the first audio attribute indicates a first condition of a user. A second sound input may be received in the conversation. A second audio attribute may be extracted from the second sound input, wherein the second audio attribute indicates a second condition of a user. A difference between the second audio attribute and the first audio attribute is determined, wherein the difference indicates a condition change of the user from the first condition to the second condition. A response to the second sound input is generated based at least on the condition change.

Abstract: The invention provides a method for increasing the yield of 7-dehydrocholesterol in yeast by using compartmentalization. The method includes steps of: by taking yeast as a starting strain, expressing heterologous sterol delta 24-reductase and cholestenol delta-isomerase, wherein the yield of 7-DHC in Saccharomyces cerevisiae S288C is detected to be 10.15 mg/L. According to the method of the invention, partial enzymes in a 7-DHC synthesis path are positioned in compartments in Saccharomyces cerevisiae by using a peroxisome and a mitochondrial positioning tag, and a relatively independent 7-DHC synthesis path is formed. Meanwhile, the storage space of precursor substances needed by 7-DHC synthesis is increased, the feedback effect is reduced, the conversion efficiency between enzymes is improved in the same compartment, the loss of the acting substrate is reduced, and finally the yield of the 7-DHC is improved by 4 times and reaches 53.31 mg/L.