Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.

Abstract: An intelligent terminal, and a computer-readable storage medium is provided. The detection method includes: obtaining a column set, a page set, and a block set, and presetting a bad column set, a bad page set, an error threshold, and an initial bad block template; alternately obtaining bad page elements and bad column elements from the block set in sequence based on the error threshold, and alternately updating the bad page set and the bad column set in sequence; based on the bad column sets and the bad page sets corresponding to different error thresholds, updating the error threshold, obtaining a final column set from the bad column sets, and obtaining a final page set from the bad page sets; and obtaining a final bad block template. The present invention can reduce the impact of a bad page on a subsequent operation of selecting a bad column element.

Abstract: Provided herein is a method of constructing a high throughput sequencing library for use in detecting chromosome copy number variation comprising mainly the following steps: (1) subjecting DNAs to be tested to random fragmentation by a double-strand DNA fragmentation enzyme; (2) end-filling and adding poly-adenine at the 3?end of the fragmented DNAs; (3) connecting the end-filled DNAs having a 3?end poly-adenine with sequencing linkers to obtain connected products; (4) purifying the connected products to obtain the sequencing library; wherein steps (1)-(3) are performed in a single reaction tube. Also provided is a kit for constructing a sequencing library for use in detecting chromosome copy number variation.

Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.