Abstract: A manufacturing method of high-precision and efficient coupling FA includes: machining circular through-holes on a glass substrate, dividing the glass substrate, and cutting a glass substrate upper surface to obtain an array of V-shaped grooves; peeling a coating layer at an end of each optical fiber, washing and placing them into the array of V-shaped grooves, covering a glass cover to obtain a first intermediate FA; inserting ceramic sleeves into the first intermediate FA to obtain a second intermediate FA; cutting the second intermediate FA to obtain a first FA and a second FA, and grinding and coating; inserting ceramic ferrules into the ceramic sleeves in the first FA, so that an end of the ceramic ferrule is exposed from the first FA, and fixing them; and inserting the end of the ceramic ferrule exposed from the first FA into the second FA for coupling to obtain a finished FA.

Abstract: Provided are a Cas effector protein, a fusion protein containing said protein, and a nucleic acid molecule coding same. Also provided are a complex and a composition for nucleic acid editing, for example, a complex and a composition for gene or genome editing, containing the Cas effector protein or the fusion protein, or the nucleic acid molecule encoding same. Also provided is a method for nucleic acid editing, for example, a method for gene or genome editing, using the Cas effector protein or the fusion protein.

Abstract: The application belongs to the field of nucleic acid editing, in particular to the field of clustered regularly interspaced short palindromic repeats (CRISPR) technology. In particular, the application provides a Cas effector protein, a fusion protein with the Cas effector protein, and a nucleic acid molecule encoding the same. Also provided are a compound and a composition for nucleic acid editing (e.g., gene or genome editing) with the protein or the nucleic acid molecule, and a method for nucleic acid editing (e.g., gene or genome editing) using the protein.