Abstract: A wig sheath includes a front end portion and a rear end portion. The front end portion is sleeved at a front end of a user head, the rear end portion is sleeved at a rear end of the user head, a plurality of mesh holes are formed in the front end portion and the rear end portion, and the mesh holes are used for fixing the wig. The rear end portion is provided with a fastener. An edge portion is formed at a lower end of the front end portion, and the edge portion fits with the user hairline. When wearing the wig sheath, the user can sleeve the front end portion of the head and stretch the fastener, and sleeve the rear end portion at the head. An edge portion is aligned with the head hairline of the user by regulating the position of the wig sheath.

Abstract: The disclosure provides Cas12f polypeptides, fusion proteins comprising such Cas12f polypeptides, CRISPR-Cas12f systems comprising such Cas12f polypeptides or fusion proteins, and methods of using the same.

Abstract: The present disclosure provides a Cas12i protein (e.g., non-naturally occurring, engineered) comprising an amino acid sequence having at least about 80% (e.g., at least about any of 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%) identity to the amino acid sequence as set forth in any one of SEQ ID NOs: 1-10 (preferably, SEQ ID NOs: 1-3 and 6, and more preferably, SEQ ID NO: 1).

Abstract: The present disclosure provides a Cas12i protein (e.g., non-naturally occurring, engineered) comprising an amino acid sequence having at least about 80% (e.g., at least about any of 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%) identity to the amino acid sequence as set forth in any one of SEQ ID NOs: 1-10 (preferably, SEQ ID NOs: 1-3 and 6, and more preferably, SEQ ID NO: 1).