Abstract: Methods and systems are provided that receive biological trait information of a subject and biological trait information of controls; and generate artificial image objects (AIOs) from the biological trait information, where each AIO is formed of an array of cells that are single unit addressable (e.g., x,y coordinates). Each cell of the AIO for the subject and the AIOs for the controls is accorded a specific graphic pixel signal corresponding to at least one data type of specific variant information of an assigned discrete unit of the biological trait information for that cell. The AIOs for the controls form a training set of artificial image objects, which are used to train an artificial intelligence (AI) algorithm for classifying AIOs. The trained AI algorithm is applied to the AIO for the subject to determine a probability that a particular biological trait is present in the subject.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for training a neural network to perform a machine learning task using a momentum and sign based optimizer.

Abstract: Methods and systems are provided for classifying genetic variant and gene function and/or expression data, as well as DNA methylation, epigenomics, proteomics, metabolomics, microbiomics, and other biological/omics data into one or more uni- or multi-dimensional artificial image objects (AIOs) for image analyses. AIOs are composed of a plurality of cells, each being assigned a specific variant. Each variant is assigned a specific value. The graphic pixel signals from AIOs generated from a population of subjects each possessing a particular trait (or not) are analyzed and/or trained collectively with Machine Learning (ML) or other Artificial Intelligence (AI) algorithms. The trained algorithm then detects characteristic signatures of the trait from the AIO to determine whether a subject possesses the trait or not, thereby affording rapid and accurate detection and better treatment. Traits include, but are not limited to, diseases such as mental illness, cancer, heart disease, and other biological conditions.

Abstract: It discloses an acoustic channel-based data communications method which performs channel coding on an original data signal using a CRC coding method and a BCH coding method to obtain a coded sequence; modulates the coded sequence using a preset audio sequence symbol set via a symbol mapping method to obtain a digital audio signal; selects a channel frequency band according to characteristics of a transmitting equipment and interference between frequency bands; and converts the digital audio signal into an analog audio signal through a digital-to-analog converter and transmits the signal to a channel for transmission according to the selected channel frequency band.

Abstract: Disclosed are a method and a system for remote wake-up and status check, with a wake-up signal generating unit generating and transmitting a wake-up signal to wake up an electricity supply unit of a remote device to operate normally and provide electricity for the remote device. The wake-up signal has an assigned electric voltage with specific characteristics for detection by the electricity supply unit while in power-off mode and, upon signal detection, the electricity supply unit wakes up to provide normal electric voltage output. The wake-up signal generating unit determines, according to an electric current on a wake-up signal feed line, whether the remote device is in an active mode. A low wake-up energy consumption enables system wake-up and a true powered-off sleep mode.

Abstract: Disclosed are a method and a system for remote wake-up and status check, with a wake-up signal generating unit generating and transmitting a wake-up signal to wake up an electricity supply unit of a remote device to operate normally and provide electricity for the remote device. The wake-up signal has an assigned electric voltage with specific characteristics for detection by the electricity supply unit while in power-off mode and, upon signal detection, the electricity supply unit wakes up to provide normal electric voltage output. The wake-up signal generating unit determines, according to an electric current on a wake-up signal feed line, whether the remote device is in an active mode. A low wake-up energy consumption enables system wake-up and a true powered-off sleep mode.

Abstract: Disclosed are a wired long-distance constant-voltage electricity-feeding method with a wake-up function and a system. A smart electricity supply module of a central electricity supply device generates a feed voltage from a central electricity source, and feeds the voltage to a terminal electricity source module through a feed line. Said smart electricity supply module can continuously provide electricity at a constant voltage to the terminal electricity source module and can change feed voltage polarity according to set rules when the terminal electricity module in sleep-mode must be remotely waken up. A voltage polarity monitoring module of said terminal electricity source module can determine, by monitoring the polarity of the voltage of the centrally fed electricity, whether to wake up the terminal electricity source module from sleep-mode to enter a normal electricity-supplying mode.

Abstract: High throughput methods and kits for single nucleotide polymorphism (SNP) genotyping are provided. The methods involve utilizing nested PCR amplification reactions which produce sequencible and ligatible structures. An outer PCR primer set amplifies the SNP, and an inner PCR primer set amplifies a portion of the DNA amplified by the outer primer set, but does not amplify the SNP itself. The inner and outer primers may reaction include non-target common domain sequences, and the inner primer common domain sequences may comprise digestion restriction endonuclease recognition sites. The design of the inner primer set allows precise tailoring of the sequencible and ligatible structures with respect to length and base composition.

Abstract: A method for single nucleotide polymorphism (SNP) genotyping using widely available DNA sequencers is provided. A restriction endonuclease recognition site is incorporated into a PCR primer for the SNP, a restriction enzyme is used to cleave the DNA and create extendable ends at target polymorphic sites, and an extension reaction is used to create allele-specific extension products that can be distinguished using DNA sequencers or other detection platforms.

Abstract: High throughput methods and kits for single nucleotide polymorphism (SNP) genotyping are provided. The methods involve utilizing nested PCR amplification reactions which produce sequencible and ligatible structures. An outer PCR primer set amplifies the SNP, and an inner PCR primer set amplifies a portion of the DNA amplified by the outer primer set, but does not amplify the SNP itself. The inner and outer primers may reaction include non-target common domain sequences, and the inner primer common domain sequences may comprise digestion restriction endonuclease recognition sites. The design of the inner primer set allows precise tailoring of the sequencible and ligatible structures with respect to length and base composition.

Abstract: Methodology for a two-level allele-specific extension (AS-PCR) reaction for use in ultra-high throughput genotyping is provided. A primary AS-PCR reaction is carried out with primers containing both sequence-specific and artificial, universal domains. The primers are designed to render the PCR products 1) allele specific and 2) amenable to amplification with secondary primers which also contain universal domains. The secondary primers are designed to maintain allele-specificity, and to provide a detectable label.

Abstract: A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.

Abstract: A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.

Abstract: A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

Abstract: A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.