Abstract: The present disclosure discloses a protective film, a display module, a display assembly, a method for preparing the display assembly and a display device. The protective film is configured to be attached to a surface on a display side of the flexible display substrate before a mother set is cut. The protective film includes a base material layer, a first adhesive layer and a second adhesive layer, wherein the first adhesive layer is located on the side, facing the flexible display substrate, of the base material layer; the viscosity of the first adhesive layer is 0.5 gf/inch to 1.5 gf/inch; the second adhesive layer is located on the side, facing the flexible display substrate, of the base material layer, is independently arranged relative to the first adhesive layer; and the viscosity of the second adhesive layer is 3 gf/inch to 4 gf/inch.