Abstract: The present invention provides a method and apparatus for system lineup and testing (SLAT) that is suitable for practical high-density cross-connect implementation. Preferably, a software assistant (wizard) guides a user through the various phases of SLAT in an automated fashion, and is able to co-ordinate tasks in a SLAT session possibly being performed at a plurality of locations such that essential tasks in said SLAT session are completed in proper sequence and some tasks of said SLAT session are performed in parallel. The present invention advantageously provides SLAT with the following benefits: support for both local and remote users; supports for multiple users; facility for users to collaborate; production of a report that is saved for reference, providing a persistent record of session data; permitting users to quit, resume and continue an incomplete session; reporting errors to users; SLAT that may be run locally (stand-alone) or remotely (on a server).

Abstract: Methods, compositions, and kits for the detecting and/or quantification of polynucleotides are provided. An exemplary method provides hybridizing a first primer to the target polynucleotide and forming equal length primer extension products using a nucleotide reaction mixture consisting of X, Y, and Z, wherein X and Y are different purine non-terminator nucleotides, and Z is a pyrimidine non-terminator nucleotide, or X and Y are different pyrmidine non-terminator nucleotides, and Z is a purine non-terminator nucleotide. A portion of the extension products are hybridized to a second primer that is immobilized on a solid support, and the second primer is extended with at least one nucleotide having a detectable marker. The signal from the detectable marker can be correlated to the amount of target polynucleotide. Kits for performing this method are also provided.

Abstract: The present invention provides a method and apparatus for system lineup and testing (SLAT) that is suitable for practical high-density cross-connect implementation. Preferably, a software assistant (wizard) guides a user through the various phases of SLAT in an automated fashion, and is able to co-ordinate tasks in a SLAT session possibly being performed at a plurality of locations such that essential tasks in said SLAT session are completed in proper sequence and some tasks of said SLAT session are performed in parallel. The present invention advantageously provides SLAT with the following benefits: support for both local and remote users; supports for multiple users; facility for users to collaborate; production of a report that is saved for reference, providing a persistent record of session data; permitting users to quit, resume and continue an incomplete session; reporting errors to users; SLAT that may be run locally (stand-alone) or remotely (on a server).