Abstract: This document relates to materials and methods for the production of protein. In one aspect, this document provides a nucleic acid construct including a first alcohol oxidase promoter element, wherein the first alcohol oxidase promoter element includes a mutation at one or more nucleotide positions corresponding to any of nucleotide positions 668-734 relative to SEQ ID NO: 28.

Abstract: A high-temperature and high-pressure microscopic visual flowing device and an experimental method are provided by the present disclosure, comprising a seepage simulation system, a micro-displacement and metering system connected to the seepage simulation system, and an image acquisition and analysis system; the seepage simulation system consists of a visual high-temperature and high-pressure kettle, a microscopic core model placed in the visual high-temperature and high-pressure kettle, and glass carriers arranged above and below the microscopic core model; the glass carriers are provided with sealing rubber sleeves, and the visual high-temperature and high-pressure kettle is provided with an annular heating jacket; an outlet of the microscopic core model is provided with a microflow channel which is connected to the micro-displacement and metering system through a pipe, effectively reducing the metering error caused by the dead volume of the pipe.

Abstract: A high-temperature and high-pressure microscopic visual flowing device and an experimental method are provided by the present disclosure, comprising a seepage simulation system, a micro-displacement and metering system connected to the seepage simulation system, and an image acquisition and analysis system; the seepage simulation system consists of a visual high-temperature and high-pressure kettle, a microscopic core model placed in the visual high-temperature and high-pressure kettle, and glass carriers arranged above and below the microscopic core model; the glass carriers are provided with sealing rubber sleeves, and the visual high-temperature and high-pressure kettle is provided with an annular heating jacket; an outlet of the microscopic core model is provided with a microflow channel which is connected to the micro-displacement and metering system through a pipe, effectively reducing the metering error caused by the dead volume of the pipe.

Abstract: A touch panel includes a first substrate, a liquid crystal layer, a second substrate, a first optical detection layer, and a black matrix. The first optical detection layer is over a light-emitting surface of the liquid crystal layer and includes a plurality of first optical detection components, whose orthographic projections on the first substrate are within an orthographic projection of the black matrix. A touch control can be determined based on a change of a first electric signal converted by each first optical detection component based on an intensity of a light transmitting through the liquid crystal layer. The light can be an infrared light. A plurality of second optical detection components can be further disposed over a light-incident surface of the liquid crystal layer to pairingly correspond to, and utilized to determine a touch control along with, the plurality of first optical detection components.

Abstract: A computer system can determine historical interval data of a freight operator from completed freight service requests associated with the freight operator. Additionally, while the freight operator is fulfilling a current freight service request, the computer system can determine at least a first likely downtime interval for a non-operating activity of the freight operator prior to the freight operator arriving at a pickup location or a destination location of the current freight service request, based on the historical interval data. Also, the computer system can determine an estimated arrival time for the freight operator to arrive at the pickup location or the destination location of the current freight service request, based at least in part on the first likely down time interval. Moreover, the computer system can update a record associated with the freight operator to reflect the estimated arrival time.

Abstract: A test system includes a transmitter, capacitor, and receiver. The transmitter includes: an output circuit coupled to the receiver via the capacitor; a transmitting circuit transmitting a predetermined signal to a pad of the output circuit during a first test; a signal generator outputting a first signal and second signal to the pad during a first process and second process of a second test; and a comparator comparing the pad's signal with a reference signal in the first process and second process to determine whether the second test passes. The receiver includes: an input circuit coupled to the transmitter via the capacitor; a switch coupled between the input circuit and a receiving circuit to be conducting in the first test and second process and nonconducting in the first process; and the receiving circuit determining whether the first test passes according to the predetermined signal and assisting the capacitor in discharging.

Abstract: The present invention disclosed a method for preparing a thickener for tight oil and gas reservoir, which relieves damages. The technical solution includes the following steps: putting a three-flask in ice; adding perfluoroalkyl alcohol, P-toluenesulfonyl chloride and pyridine; reacting for 3 h at 0-20° C.; adding filter paper; ultrasonic dispersing for 1 h; removing the filter paper; washing the solution for 3-5 times by adding dilute hydrochloric acid to collect the intermediate product (I); adding 1,3-dihydroxy-propane-2-tert-butyl carbamate and the intermediate product (I) in another three-flask; adding potassium carbonate and N,N-dimethylformamide; reacting for 4 h at 40° C. to collect the intermediate product (II); adding trifluoroacetic acid and methylene chloride into the intermediate product (II); reacting at 45° C. for 2 h; extracting and vacuum drying; and adding 1,6-hexamethylene diisocyanate; reacting for 2 h to collect the final product (III).

Abstract: A computer system can determine historical interval data of a freight operator from completed freight service requests associated with the freight operator. Additionally, while the freight operator is fulfilling a current freight service request, the computer system can determine at least a first likely downtime interval for a non-operating activity of the freight operator prior to the freight operator arriving at a pickup location or a destination location of the current freight service request, based on the historical interval data. Also, the computer system can determine an estimated arrival time for the freight operator to arrive at the pickup location or the destination location of the current freight service request, based at least in part on the first likely down time interval. Moreover, the computer system can update a record associated with the freight operator to reflect the estimated arrival time.

Abstract: The disclosure relates to genetic engineering, and more particularly to a CHO cell line, its construction and a recombinant protein expression system using the CHO cell line. Regardless of the presence screening pressure, the recombinant protein expression system constructed by the 60th passage of the recombinant APRT gene-deficient CHO cell line is much higher than the normal recombinant CHO cell in the expression retention of the target protein EGFP. In the absence of G418 screening pressure, the expression level of the recombinant vitronectin in the recombinant protein expression system constructed by the 30th passage of the recombinant APRT gene-deficient CHO cell is significantly higher than the expression level of the corresponding target protein in the normal recombinant CHO cells.

Abstract: A light source device, a backlight module and a liquid crystal display device are provided. The backlight module includes a prism structure, wherein the prism structure includes a substrate, prismatic tooth portions and a transparent sealing portion which are arranged in a stacked manner; and the transparent sealing portion seals the prismatic tooth portions, and the space between the prismatic tooth portions and the transparent sealing portion is a vacuum space.

Abstract: The present disclosure relates to the field of touch display technology, and in particular, provides a touch display apparatus and a driving method therefor. Specifically, the touch display apparatus includes a first substrate and a second substrate opposite to each other, a light source on a side of the second substrate facing the first substrate, a plurality of touch sensors in an array on a side of the first substrate facing the second substrate, and one or more interference color filters on a side of each touch sensor facing the second substrate. Specifically, the light source is configured to emit light towards each interference color filter. Further, each interference color filter comprises a first sub-interference color filter, a second sub-interference color filter and a third sub-interference color filter configured to output different colors of light respectively.

Abstract: Provided are a memory lymphocyte population, a culture medium, and a method for obtaining the memory lymphocyte population and a use of the same. The memory lymphocyte population contains at least one of the following marker molecules: leukocyte differentiation antigens CD3, CD4, CD8, CD16, CD56, CD62L and CD45RO.

Abstract: A touch panel includes a first substrate, a liquid crystal layer, a second substrate, a first optical detection layer, and a black matrix. The first optical detection layer is over a light-emitting surface of the liquid crystal layer and includes a plurality of first optical detection components, whose orthographic projections on the first substrate are within an orthographic projection of the black matrix. A touch control can be determined based on a change of a first electric signal converted by each first optical detection component based on an intensity of a light transmitting through the liquid crystal layer. The light can be an infrared light. A plurality of second optical detection components can be further disposed over a light-incident surface of the liquid crystal layer to pairingly correspond to, and utilized to determine a touch control along with, the plurality of first optical detection components.

Abstract: The present disclosure relates to a polarizer and a display device. The polarizer includes a polarization layer, a color filter layer located on the polarization layer, wherein the color filter layer includes a quantum dot. The application of the polarizer provided by the embodiments of the present disclosure to the display device can increase the color gamut, the color purity, and the light utilization, thereby improving the display color quality.

Abstract: A test system includes a transmitter, capacitor, and receiver. The transmitter includes: an output circuit coupled to the receiver via the capacitor; a transmitting circuit transmitting a predetermined signal to a pad of the output circuit during a first test; a signal generator outputting a first signal and second signal to the pad during a first process and second process of a second test; and a comparator comparing the pad's signal with a reference signal in the first process and second process to determine whether the second test passes. The receiver includes: an input circuit coupled to the transmitter via the capacitor; a switch coupled between the input circuit and a receiving circuit to be conducting in the first test and second process and nonconducting in the first process; and the receiving circuit determining whether the first test passes according to the predetermined signal and assisting the capacitor in discharging.

Abstract: The invention relates to genetic engineering, and more particularly to a Dnmt3b gene-deficient CHO cell line, a preparation method and an application thereof and a recombinant protein expression system using the same. The invention adopts a CRISPR/Cas9 gene editing technique to knock out the Dnmt3b gene from the CHO cells to produce the Dnmt3b gene-deficient CHO cell line, which can significantly improve the expression level and stability of the target gene in CHO cells, overcoming the defects existing in the current CHO cell expression system, such as low expression level and stability. It has been demonstrated that using the CHO line provided herein to express a recombinant adalimumab can significantly increase the expression level of the recombinant adalimumab, indicating that the CHO cell line can be widely used to enhance the expression of target proteins.

Abstract: Disclosed are a conductive particle, an anisotropic conductive film, a display device, and a method for fabricating the same so as to detect the extent to which the conductive particles are cracked in a heating and pressurizing process, to thereby improve the ratio of finished products while the display device is being manufactured. A core of the conductive particle is a fluorescent resin core. In the conductive particle according to this disclosure, the core of the conductive particle is a fluorescent resin core, and the extent to which the conductive particle is cracked can be detected by detecting varying fluorescence in a heating and pressuring process, to thereby alleviate such a phenomenon from taking place that the conductive particle has a poor electrical conductivity due to an insufficient pressure, or the conductive particle is cracked, and thus loses its electrical conductivity, due to an excessive pressure.

Abstract: The present invention disclosed a method for prparing a thickener for tight oil and gas reservoir, which relieves damages. The technical solution includes the following steps: putting a three-flask in ice; adding perfluoroalkyl alcohol, P-toluenesulfonyl chloride and pyridine; reacting for 3 h at 0-20° C.; adding filter paper; ultrasonic dispersing for 1 h; removing the filter paper; washing the solution for 3-5 times by adding dilute hydrochloric acid to collect the intermediate product (I); adding 1,3-dihydroxy-propane-2-tert-butyl carbamate and the intermediate product (I) in another three-flask; adding potassium carbonate and N,N-dimethylformamide; reacting for 4h at 40° C. to collect the intermediate product (II); adding trifluoroacetic acid and methylene chloride into the intermediate product (II); reacting at 45° C. for 2 h; extracting and vacuum drying; and adding 1,6-hexamethylene diisocyanate; reacting for 2 h to collect the final product (III).

Abstract: This document relates to materials and methods for the production of protein. In one aspect, this document provides a nucleic acid construct including a first alcohol oxidase promoter element, wherein the first alcohol oxidase promoter element includes a mutation at one or more nucleotide positions corresponding to any of nucleotide positions 668-734 relative to SEQ ID NO: 28.

Abstract: The present disclosure relates to the field of touch display technology, and in particular, provides a touch display apparatus and a driving method therefor. Specifically, the touch display apparatus includes a first substrate and a second substrate opposite to each other, a light source on a side of the second substrate facing the first substrate, a plurality of touch sensors in an array on a side of the first substrate facing the second substrate, and one or more interference color filters on a side of each touch sensor facing the second substrate. Specifically, the light source is configured to emit light towards each interference color filter. Further, each interference color filter comprises a first sub-interference color filter, a second sub-interference color filter and a third sub-interference color filter configured to output different colors of light respectively.