Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Abstract: The present application discloses a dispatching and control cloud data processing method, device and system. The method includes the following operations: pilot node device acquires a global scheduling task, decomposes the global scheduling task to obtain scheduling tasks, issues the scheduling tasks to collaborative node device, acquires data collection ranges and data processing rules of the collaborative node devices, and delivers them to the collaborative node devices; the collaborative node devices receive and execute the scheduling tasks issued by the pilot node device; receives the data collection ranges and the data processing rules issued by the pilot node device, acquires, based on the scheduling tasks, collected data in the data collection ranges, processes the acquired collected data according to the data processing rules to obtain the processed data, uploads the processed data to the pilot node device; the pilot node device receives the processed data uploaded by the collaborative node devices.

Abstract: Provided is a method for interplanting grape and Polygonatum kingianum in a photovoltaic (PV) power plant. The method includes: building a grapevine traction frame which is higher in south and lower in north in a space formed by a plurality of PV modules in the PV power plant, the grapevine traction frame includes a plurality of uprights, a plurality of traction crossbars, a plurality of supporting bars and a wire mesh; making a grape ridge below the south of each PV module and planting grape seedlings; maintaining two branches for each of the grape seedlings in the same year of grape planting, and pulling the two branches along the traction crossbar located in south; pulling branches grown out of arm bud points southwards onto the wire mesh in the next year of grape planting; sowing and ploughing a green manure, making planting beds in a shade and planting Polygonatum kingianum.

Abstract: Reusable flow cells for sequencing which exhibit signal intensity retention over numerous use cycles, the active surface of which contains poly-azide functional moieties, methods of treating flow cells surfaces with reagents to provide such poly-azide functional moieties, and reagents therefor.

Abstract: The disclosure relates to KRASG12D inhibitor compounds having the structure of Formula (A) or Formula (B), pharmaceutical compositions thereof, and methods of use thereof for inhibiting, treating, and/or preventing KRASG12D mutation-associated diseases, disorders and conditions.

Abstract: An interaction method, an interaction apparatus, and an electronic device are provided. The method includes: determining, in response to detecting a predefined size transformation operation, target transformation information of a target video based on whether a current size of the target video is a preset anchor point size, where the target video is a video played in a video play area; and transforming the target video based on the target transformation information, and playing the transformed target video.

Abstract: The present invention relates to application of gossypol and its optical isomers to preparation of an anti-Coronavirus drug. Specifically disclosed is application of pharmaceutically acceptable salts, solvates, isotopes, stereoisomers, stereoisomer mixtures, and tautomers of the gossypol and its optical isomers to preparation of a drug for preventing and/or treating a disease caused by a Coronavirus. The Coronavirus is MERS-CoV, SARS-CoV, or SARS-CoV-2. It is found in the present invention for the first time that the gossypol and its optical isomers can inhibit the activity of Coronavirus 3CL proteases to achieve an anti-Coronavirus effect. The half maximal inhibitory concentrations of gossypol and (?)-gossypol to SARS-CoV proteases and SARS-CoV-2 3CL proteases are all lower than 10 ?M. The anti-Coronavirus 3CL protease effect is good. Therefore, the gossypol and its optical isomers have the potential for use in the preparation of a drug for preventing and/or treating novel Coronavirus infections.

Abstract: The present application relates to secondary amine-substituted coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Abstract: The present invention relates to a digital CRISPR-based method for detecting and quantitating target nucleic acids in a sample comprising: forming a mixture comprising sample nucleic acids; isothermal amplification reaction reagents for amplifying one or more target nucleic acid sequences; partitioning the mixture into a plurality of compartments; incubating the partitioned mixture at a temperature for isothermal amplification and Cas effector cleavage of an amplified DNA strand, detecting a signal from cleavage of the non-target sequence, thereby detecting the one or more target sequences in the sample, and determining the copy number of the target nucleic acid based on a Poisson distribution of the proportion of positive-to-negative compartments. The invention also relates to a method for detecting presence and/or of a disease in a subject, and a kit to quantitate nucleic acids in a sample.

Abstract: Embodiments of the present disclosure relate to kits, compositions, and methods for nucleic acid sequencing, for example, two-channel nucleic acid sequencing by synthesis using blue and green light excitation. In particular, unlabeled nucleotides for incorporation may be used in conjunction with affinity reagents containing detectable labels excitable by blue and/or green lights, for specific binding to each type of nucleotides incorporated.

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Abstract: Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3? AOM blocking group. Also provided herein are kits related to such methods.

Abstract: Embodiments of the present disclosure relate to compositions and kits for use in sequencing by synthesis to improve fluorescent signal intensity and reduce signal decay caused by short wavelength light source during the imaging events. Methods of sequencing using the compositions and kits described herein are also provided.

Abstract: A filter capable of automatically cutting off gas flow, including: a housing (1) with a cavity, a filter element (2) disposed in the cavity, a blocking mechanism disposed in the flow channel (13), and an unblocking member (23). The housing is provided with an inlet end (11) and an outlet end (12). The filter element (2) is provided with a first chamber (21). A second chamber (23) is formed between the filter element (2) and the housing (1), and a flow channel (13) is disposed between the first chamber (21) and the inlet end (11). The blocking mechanism has an unblocking state in which the blocking mechanism is cooperated with the unblocking member (23) to allow a gas flow to flow out of the flow channel (13), and a blocking state in which the blocking mechanism prevents the gas flow from flowing out of the flow channel (13).

Abstract: Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

Abstract: A method, device and computer-readable medium for generating a super-resolution version of a compressed video stream. By leveraging the motion information and residual information in compressed video streams, described examples are able to skip the time-consuming motion-estimation step for most frames and make the most use of the SR results of key frames. A key frame SR module generates SR versions of I-frames and other key frames of a compressed video stream using techniques similar to existing multi-frame approaches to VSR. A non-key frame SR module generates SR version of the non-key inter frames between these key frames by making use of motion information and residual information used to encode the inter frames in the compressed video stream.

Abstract: Embodiments of the present disclosure relate to compositions and methods for improving the intensity of the fluorescent signals during nucleic acid sequencing. In particular, at least one biotin-binding site of the labeled streptavidin is blocked to reduce fluorescent signal deflation.

Abstract: Disclosed are a genetic tumor marker combination, a methylation detection reagent, a kit, and use thereof. Lung cancer specimens can be distinguished by measuring the methylation level of a combination of HOXB4 and SRCIN1 genes. Proved by experiments, the reagent of the present disclosure can detect and diagnose lung cancer, and has clinical application value.

Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.