Patents by Inventor Xiaolin Wu

Xiaolin Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250146066
    Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Application
    Filed: January 13, 2025
    Publication date: May 8, 2025
    Inventors: Antoine FRANCAIS, Elena CRESSINA, Adam CULLEY, Angelica MARIANI, Xiaolin WU, Xiaohai LIU
  • Patent number: 12267196
    Abstract: Provided are a method for processing an OFDM signal, a system for transmitting an OFDM signal, and a device, which effectively reduce a peak to average power ratio of the OFDM signal.
    Type: Grant
    Filed: November 17, 2023
    Date of Patent: April 1, 2025
    Assignee: CHONGQING SATELITE NETWORK SYSTEM CO., LTD.
    Inventors: Linhai Gu, Guangnan Zou, Xuesong Wang, Xiaolin Wu
  • Publication number: 20250075269
    Abstract: Embodiments of the present disclosure relate to methods and kits for sequencing by synthesis utilizing biorthogonal chemical reactions between unlabeled nucleotides and post incorporation labeling reagents.
    Type: Application
    Filed: August 29, 2024
    Publication date: March 6, 2025
    Inventors: Xiaolin Wu, Madushani Dharmarwardana, Harry John Meats
  • Publication number: 20250069688
    Abstract: Provided herein are methods of determining loss of heterozygosity in human leukocyte antigen in liquid biopsies and applications thereof in cancer treatment.
    Type: Application
    Filed: January 13, 2023
    Publication date: February 27, 2025
    Inventors: Andrew SINKOE, James GULLEY, Christian HINRICHS, Scott Alberto NORBERG, Clint ALLEN, Nisha NAGARSHETH, Xiaolin WU, Ling SU
  • Patent number: 12227802
    Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Grant
    Filed: October 17, 2023
    Date of Patent: February 18, 2025
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Patent number: 12195796
    Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.
    Type: Grant
    Filed: May 2, 2023
    Date of Patent: January 14, 2025
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu, Nikolai Nikolaevich Romanov, Antoine Francais, Patrick McCauley, Michael Callingham, Carole Anastasi
  • Publication number: 20240400605
    Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3? phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
    Type: Application
    Filed: August 15, 2024
    Publication date: December 5, 2024
    Inventors: Xiaolin Wu, Randall Smith, Peyton Shieh, John M. Beierle, Wayne N. George, Elliot John Lawrence, Jie Mao, Xiaohai Liu
  • Publication number: 20240327909
    Abstract: Embodiments of the present application relates to methods and compositions for increasing fluorescent dye signal intensity during sequencing by synthesis. In particular, the compositions and methods described herein involve the use aqueous scan mixtures including one or more water-soluble macrocycles that can form host-guest complexes with the dyes.
    Type: Application
    Filed: March 27, 2024
    Publication date: October 3, 2024
    Inventors: Xiaolin Wu, Pietro Gatti Lafranconi, Timothy Beech, Carlo Bravin, Benedict Mackworth, Thomas Tongue, Philip Balding
  • Publication number: 20240309423
    Abstract: Automated methods conducted in a sequencing flowcell, and kits for reusing a flowcell, are provided herein. In some examples, an automated method conducted in a sequencing flowcell may include, at a surface of the sequencing flowcell coupled to a first moiety, using a reagent to decouple a first complex from the first moiety. In some examples, the first complex may include a second moiety which couples to the first moiety and a polynucleotide coupled to the second moiety. In some examples, the method may further include using a nuclease to polynucleotides in the sequencing flowcell. The method may include, after using the reagent and after using the nuclease, coupling a second complex to the first moiety. The second complex may include a third moiety which couples with the first moiety and an oligonucleotide coupled to the third moiety.
    Type: Application
    Filed: March 5, 2024
    Publication date: September 19, 2024
    Applicant: Illumina, Inc.
    Inventors: Jonathan Boutell, Katharina Mueller-Ott, Jason Betley, Xiaolin Wu, Wayne George, Pietro Gatti Lafranconi, Andrew Brown
  • Publication number: 20240300896
    Abstract: Described herein are heterocyclic azide-containing monomer units, copolymers comprising such heterocyclic azide-containing monomer units, substrate-bound copolymers, and oligonucleotide-bound copolymers, methods for making such copolymers and reacting them with a substrate and/or oligonucleotide, and methods of using such copolymers for immobilization of oligonucleotides to a substrate, for example for use in DNA sequencing or other diagnostic applications.
    Type: Application
    Filed: May 31, 2024
    Publication date: September 12, 2024
    Inventors: Wayne N. George, Xiaolin Wu, Andrew A. Brown, Donald Wellings
  • Patent number: 12084474
    Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3? phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: September 10, 2024
    Assignee: Illumina, Inc.
    Inventors: Xiaolin Wu, Randall Smith, Peyton Shieh, John M. Beierle, Wayne N. George, Elliot John Lawrence, Jie Mao, Xiaohai Liu
  • Publication number: 20240294967
    Abstract: Embodiments of the present disclosure relates to various bisulfite-free chemical methods for detecting methylation of cytosine in the DNA sample. These methods convert methylated and hydroxymethylated cytosine in the nucleic acid sequence to a modified or pseudo thymine or a uracil moiety which then can be detected in sequencing.
    Type: Application
    Filed: January 18, 2023
    Publication date: September 5, 2024
    Inventors: Xiaolin WU, Antoine FRANCAIS, Xiaohai LIU
  • Publication number: 20240271206
    Abstract: Embodiments of the present disclosure relate to nucleotides with 3? allyl blocking groups. Also provided herein are methods of sequencing using nucleotides with 3? allyl blocking groups described herein, and sequencing kits.
    Type: Application
    Filed: December 21, 2023
    Publication date: August 15, 2024
    Inventors: Antoine Francais, Elena Cressina, Kathryn Hattingh, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240271209
    Abstract: The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: April 9, 2024
    Publication date: August 15, 2024
    Inventors: Nikolai Nikolaevich Romanov, Michael Callingham, Carole Anastasi, Patrick McCauley, Niall Hynes, Natasha Crake, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240271185
    Abstract: Disclosed herein include methods, compositions, reaction mixtures, kits and systems for identification of methylated cytosines in nucleic acids using a bisulfite-free, one-step chemoenzymatic modification of methylated cytosines.
    Type: Application
    Filed: August 16, 2022
    Publication date: August 15, 2024
    Inventors: Colin Brown, Xiaohai Liu, Xiaolin Wu, Eric Brustad, Sarah E. Shultzaberger
  • Publication number: 20240254577
    Abstract: The present disclosure relates to a spheroidizing-annealed steel for the ball screw having high strength and resistance to low temperatures, wherein the chemical composition of the steel in mass percentage is: C: 0.40-0.70%, Si: 1.20-1.80%, Mn: 1.00-1.60%, Cr: 0.80-1.20%, S: ?0.025%, P?0.025%, Ni: 0.10-0.60%, Cu: 0.30-0.80%, Mo: 0.10-0.40%, Al?0.05%, Ca?0.0010%, Ti?0.003%, O?0.0010%, As?0.04%, Sn?0.03%, Sb?0.005%, Pb?0.002%, the balance is Fe and unavoidable impurities. In microstructure of the steel, cementite exists in a spheroidized state with a diameter of 0.1-0.5 ?m, preferably 0.3-0.5 ?m, a spheroidizing rate is 95% or more, and the rest is ferrite.
    Type: Application
    Filed: April 11, 2024
    Publication date: August 1, 2024
    Applicant: JIANGYIN XINGCHENG SPECIAL STEEL WORKS CO., LTD
    Inventors: Jiaolong ZHAI, Yun BAI, Qihang FAN, Xiaolin WU, Qian LIU, Shuyan SHAO, Yun LI, Kui ZHANG, Zelei CHEN, Qian LI, Lei GAO, Yu MENG, Shuquan LIAO, Sha LU
  • Patent number: 12031178
    Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.
    Type: Grant
    Filed: February 14, 2023
    Date of Patent: July 9, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu, Nikolai Nikolaevich Romanov, Antoine Francais, Patrick McCauley, Michael Callingham, Carole Anastasi
  • Publication number: 20240218443
    Abstract: The present application relates to palladium compositions, methods for sequencing by synthesis using nucleotides with 3? blocking groups, and sequencing kits, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.
    Type: Application
    Filed: December 21, 2023
    Publication date: July 4, 2024
    Inventors: Angelica Mariani, Antoine Francais, Frederick James Topping, Christopher Winnard, Philip Balding, Chol Steven Yun, Patrizia Iavicoli, Kathryn Hattingh, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240209015
    Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal or allyl 3? blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3? blocking groups for sequencing applications.
    Type: Application
    Filed: December 21, 2023
    Publication date: June 27, 2024
    Inventors: Antoine Francais, Elena Cressina, Angelica Mariani, Adam Culley, Anno Koetje, Xiaohai Liu, Xiaolin Wu, Kathryn Hattingh
  • Publication number: 20240182963
    Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3? acetal, thiocarbamate or allyl blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Application
    Filed: December 21, 2023
    Publication date: June 6, 2024
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu, Kathryn Hattingh