Abstract: In one aspect, compositions and methods for reducing reverse transcriptase (RT) inhibition in RT-PCR are provided. In some embodiments, the RT inhibition reducer is a phosphorothioate oligodeoxycytosine (SdC), phosphorothioate oligodeoxyadenine (SdA), phosphorothioate oligodeoxythymine (SdT), or phosphorothioate oligodeoxyguanosine (SdG).

Abstract: Amplification reaction mixtures comprising a cationic surfactant and an anionic surfactant are provided.

Abstract: The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule.

Abstract: Methods, computer systems, and computer-storage medium for generating a per-document index used for semantic searching is provided. A document is received and parsed into a plurality of section. Each term in each section is translated in order to at least one of a cache index or a term identifier. Subsequent to translating the terms, each section is separately group encoded to generate the per-document index. The per-document index is stored in association with a data store.

Abstract: An approach, is presented for exchanging status updates while on and/or offline when collaborating on various activities via one or more networks. A method comprises determining one or more tasks associated with at least one collaborative activity for one or more devices participating in the at least one collaborative activity. The method also comprises determining status information for the one or more tasks with respect to the one or more devices. Further, the method also comprises causing, at least in part, an exchange of the status information among the one or more devices via at least one communication mechanism that supports off-line messaging.

Abstract: Methods, computer systems, and computer-storage medium for generating a per-document index used for semantic searching is provided. A document is received and parsed into a plurality of section. Each term in each section is translated in order to at least one of a cache index or a term identifier. Subsequent to translating the terms, each section is separately group encoded to generate the per-document index. The per-document index is stored in association with a data store.

Abstract: An infant basket comprises: a basket body; two pivot plates formed with notches; two hollow connectors pivoted to the pivot plates; two latch members mounted in the hollow connectors and operable to engage and disengage the notches; and a handle having two hollow end portions. The hollow connectors are mounted detachably in the hollow end portions such that the latch members are disposed within the hollow end portions. The latch members are co-rotatable with the handle and the hollow connectors relative to the pivot plates about a rotation axis from an upright position to a lie-back position relative to the basket body when the latch members are disengaged from the notches, respectively.

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Abstract: A computer-implemented method for debugging a program includes responding to user input to debug a program step by step. The debugging information of each line of the program is temporarily stored. Request available stored debugging information is displayed according to user input. Any stored debugging information is deleted when debugging of the program is finished.

Abstract: A database server communicates with a plurality of client devices. Each of the client devices is used to create a program package to cooperatively develop a software project. An integrating package is created in a storage of the database server. When two or more client devices simultaneously access the integrating package, a storage region is created for each of the two or more client devices in a storage space of the integrating package. When the two or more client devices write the program package to the integrating package, the program package of each of the two or more client devices is automatically written into a corresponding storage region.

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Abstract: In order to keep the advantage of PWM dimming control scheme and make the LED driver high reliability and the LED lamp long lifetime, it is required that the frequency of PWM dimming control scheme is related with or the same as the output frequency of existed dimmers; and the output PWM dimming pulse is covered by the output pulse from dimmers further. The present invention discloses a novel “LED dimming control” scheme to make that the frequency of PWM dimming control scheme is related with or the same as the output frequency of dimmers; and the output PWM dimming pulse is covered by the output pulse from the dimmers further. In this way, it can guarantee that as PWM dimming pulse comes, the output from the dimmer can offer enough power to the LED driver and the LED driver can output the required LED driving current without flicker.

Abstract: A method manages a web system having a plurality of sub-systems using a computing device. A web project is created for the web system and is stored in a storage of the computing device. Each of the sub-systems has a directory created under the web project. Programs of each sub-system are compiled to generate a dynamic link library (DLL) document of each sub-system. The DLL document of each sub-system is written into a corresponding directory under the web project.

Abstract: Methods, computer systems, and computer-storage medium for generating a per-document index used for semantic searching is provided. A document is received and parsed into a plurality of section. Each term in each section is translated in order to at least one of a cache index or a term identifier. Subsequent to translating the terms, each section is separately group encoded to generate the per-document index. The per-document index is stored in association with a data store.

Abstract: An infant basket comprises: a basket body; two pivot plates formed with notches; two hollow connectors pivoted to the pivot plates; two latch members mounted in the hollow connectors and operable to engage and disengage the notches; and a handle having two hollow end portions. The hollow connectors are mounted detachably in the hollow end portions such that the latch members are disposed within the hollow end portions. The latch members are co-rotatable with the handle and the hollow connectors relative to the pivot plates about a rotation axis from an upright position to a lie-back position relative to the basket body when the latch members are disengaged from the notches, respectively.

Abstract: Methods, computer systems, and computer-readable media for generating semantic ranking features using a forward index are provided. A search query is received and is analyzed for one or more semantic units including semantic patterns, topical categories, and entities. A forward index comprising a plurality of documents is accessed and semantic units associated with each of the documents are analyzed. The semantic units include semantic patterns, topical categories, unigrams, bigrams, and entities. Documents who share substantially similar semantic units with the search query are identified, and the ranking of the identified documents is adjusted based on the substantially similar semantic units.

Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.

Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.