Abstract: Genomic insertions, duplications, and insertion/deletion (indels) account for ˜14% of human pathogenic mutations. Current gene editing methods cannot accurately or efficiently correct these abnormal genomic rearrangements, especially larger alterations (>100 bp). The presently disclosed compositions and methods accurately delete insertions/duplications and repair the deletion junction to improve the scope of gene therapies. For example, a Cas9 prime editor (PECas9) is combined with two prime editing guide RNAs (pegRNAs) targeting complementary DNA strands. PECas9 can replace an ˜1-kb genomic fragment with a desired sequence at the target site without requiring an exogenous DNA template.

Abstract: A method and system are described for driving a set of qubits. A first qubit is provided with a first driving frequency and a second qubit is provided with a second driving frequency. Each qubit is provided with a separate microwave gate configured to apply a respective microwave signal. A first electrical signal including the first driving frequency is applied to the first microwave gate for driving the first qubit. Simultaneously a second electrical signal is applied to the second microwave gate including the first driving frequency shifted in phase with respect to the first electrical signal for generating the second microwave signal with the first driving frequency arriving at the second qubit in counterphase to first microwave signal. This may at least partially compensate crosstalk.

Abstract: A reasonable millisecond time control method for excavation blasting of a tunnel is provided, and includes: acquiring physical mechanical parameters to establish a millisecond blasting model, and designing four dimensions blasting parameters of explosive quantity, hole number, inter-hole millisecond and inter-row millisecond; simulating, based on the millisecond blasting model, a blasting process of an explosive package using blasting parameters to obtain a blasting vibration curve; obtaining single-hole blasting vibration waveforms, solving a vibration synthesis curve through a vibration synthesis theory; comparing the vibration synthesis curve with the blasting vibration curve to obtain a coupling relationship of blasting parameters; determining a target group of explosive quantity and hole numbers, determining a target millisecond through the coupling relationship of blasting parameters, and relating a millisecond blasting control strategy to control, and it is used for tunneling project to reduce cut blas

Abstract: A medical image processing apparatus according to the present embodiment includes processing circuitry. The processing circuitry extracts a registration target area corresponding to a registration target organ from an ultrasonic image by acquiring edges of the registration target organ from the ultrasonic image among a plurality of types of medical images including the registration target organ. The processing circuitry calculates the degree of deformation of a plurality of positions in the registration target area. The processing circuitry performs a rigid registration on the types of medical images on the basis of the degree of deformation for each position calculated at the positions and the similarity between the types of medical images at the positions.

Abstract: A smart sub-ambient radiative cooling composition including TiO2 particles; inorganic particles selected from the group consisting of SiO2, CaCO3, SiC, ZnO, Al2O3, ZnO, and mixtures thereof; fluorescent pigment particles; and a polymer useful for sub-ambient radiative cooling, methods of preparation, and use thereof.

Abstract: A clamp device is used for holding a plate type work-piece. The clamp device includes a base. The base includes a top surface and a bottom surface. A first positioning member and two guiding slots are formed on the base. Two movable members are located on the guiding slots, respectively. The movable member can slide along the guiding slot. The two movable members and the first positioning member form a clamping space together.

Abstract: An antihypertensive pharmaceutical composition is provided, which contains levamlodipine or a pharmaceutically acceptable salt thereof, and indapamide. In the present invention, levamlodipine and indapamide are administrated in combination for treating hypertension, a good synergistic antihypertensive effect is achieved, and the edema side effect due to sodium and water retention caused when levamlodipine is administrated alone and the side effect of glucose metabolism interference caused when indapamide is administrated alone are mitigated.

Abstract: A levamlodipine compound pharmaceutical composition is provided, which contains levamlodipine or a pharmaceutically acceptable salt thereof, and chlorthalidone. In the present invention, levamlodipine and chlorthalidone are administrated in combination for treating hypertension, a high synergistic antihypertensive effect is achieved, and the edema side effect due to sodium and water retention caused when levamlodipine is administrated alone is mitigated. In addition, levamlodipine increases insulin sensitivity of an organism, and resists the side effect of increase in blood glucose level caused by chlorthalidone. The pharmaceutical composition of levamlodipine and chlorthalidone overcomes the disadvantage that levamlodipine takes effects slowly in hypertension repression.

Abstract: An antihypertensive pharmaceutical composition is provided, which contains levamlodipine or a pharmaceutically acceptable salt thereof, and indapamide. In the present invention, levamlodipine and indapamide are administrated in combination for treating hypertension, a good synergistic antihypertensive effect is achieved, and the edema side effect due to sodium and water retention caused when levamlodipine is administrated alone and the side effect of glucose metabolism interference caused when indapamide is administrated alone are mitigated.