Abstract: A self-crosslinking recombinant humanized collagen polymer biomaterial and a preparation method therefor are provided. A collagen self-assembled element includes (i) at least 21 consecutive amino acids at the C-terminus of the amino acid sequence as shown in SEQ ID NO: 1; or an amino acid sequence that is obtained by means of substitution, deletion or addition of one or more amino acids in the amino acid sequence as described in (i) and that retains the function of promoting the self-crosslinking of recombinant type III humanized collagen to form a triple helix structure; or an amino acid sequence that has at least 90%, 92%, 95%, 96%, 97%, 98% or 99% identity to the amino acid sequence as described in (i) and that retains the function of promoting the self-crosslinking of recombinant type III humanized collagen to form a triple helix structure.

Abstract: The present application provides a preparation method for biosynthesis of a human body structural material. A polypeptide has the amino acid sequence of SEQ ID NO. 4, 5 or 6. Recombinant type-VII humanized collagen prepared in the present application has high activity of promoting cell proliferation and does not produce an immune response when applied to a human body, and the preparation method therefor is novel and can obtain the recombinant type-VII humanized collagen on a large scale, and is widely applied in the preparation of human body structural materials.

Abstract: Provided is collagen and uses thereof. Collagen includes an N-terminal sequence and a C-terminal sequence, wherein the N-terminal sequence includes one or more repeating units, and the repeating unit includes an amino acid sequence shown in SEQ ID NO. 15. The recombinant type II humanized collagen prepared by the present invention has high activity in promoting cell adhesion, does not produce an immune response when applied to the human body, can be obtained on a large scale, and is expected to be widely used in the field of cartilage repair.

Abstract: Disclosed in the present invention are a recombinant type-I humanized collagen polypeptide, and a preparation method therefor and the use thereof. The recombinant type-I humanized collagen polypeptide provided by the present disclosure comprises n repeats of a sequence as shown in SEQ ID NO: 1, wherein n is an integer greater than or equal to 1; the repetitive sequences are directly linked when n is an integer greater than or equal to 2; and optionally, the N-terminus of the recombinant type-I humanized collagen polypeptide contains an amino acid sequence capable of being cleaved off by the TEV protease. The recombinant type-I humanized collagen polypeptide provided by the present disclosure has an activity of promoting cell adhesion, the recombinant protein has an amino acid sequence selected from the amino acid sequence of natural collagen and does not generate immune response when applied to the human body, the preparation method therefor is simple, and high-yield collagen can be obtained at a low cost.

Abstract: Provided herein is a method of biosynthesizing human body structural material type IV collagen. Provided herein is a recombinant collagen including the sequence represented by SEQ ID NO: 1, etc. The recombinant collagen of the present application exhibits excellent cell-adhesion activity. The method of the present application utilizes genetic engineering techniques to produce the recombinant collagen, thereby overcoming the drawbacks of the prior art.

Abstract: Provided is a method for biosynthesis of a human body structural material type-VIII collagen. Also provided is a collagen, comprising one or more repeating units, wherein the repeating units are connected directly or by means of linkers, and each repeating unit comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, 4, 7, 10, 13, 16, 19, 22, 25 or 28, or a variant thereof. The collagen can be used for biological dressings, human body bionic materials or plastic surgery materials. According to the method, the type-VIII collagen is produced by utilizing a genetic engineering technology, thereby overcoming the defects in the prior art.

Abstract: Disclosed in the present invention is a preparation method for a biosynthetic human body structural material. The present invention provides a recombinant humanized collagen type IV, the amino acid sequence thereof comprising n repetitive sequences, the n being an integer greater than or equal to 1, and the repetitive sequences being functional region sequences of native human collagen type IV The present invention provides the amino acid sequence of the recombinant humanized collagen type IV capable of being correctly expressed in vitro and biosynthesis thereof is successfully performed in vitro. The amino acid sequence of said protein is derived from the native human collagen type IV and does not cause adverse immune reactions when the protein is applied to a human body; in addition, compared with commercial humanized collagen, the recombinant humanized collagen type IV prepared by the present invention has a higher cell adhesion effect.

Abstract: The present application provides a preparation method for biosynthesis of a human body structural material. A polypeptide has the amino acid sequence of SEQ ID NO. 4, 5 or 6. Recombinant type-VII humanized collagen prepared in the present application has high activity of promoting cell proliferation and does not produce an immune response when applied to a human body, and the preparation method therefor is novel and can obtain the recombinant type-VII humanized collagen on a large scale, and is widely applied in the preparation of human body structural materials.

Abstract: Provided is collagen and uses thereof. Collagen includes an N-terminal sequence and a C-terminal sequence, wherein the N-terminal sequence includes one or more repeating units, and the repeating unit includes an amino acid sequence shown in SEQ ID NO. 15. The recombinant type II humanized collagen prepared by the present invention has high activity in promoting cell adhesion, does not produce an immune response when applied to the human body, can be obtained on a large scale, and is expected to be widely used in the field of cartilage repair.