Abstract: The present disclosure discloses an autophagosome for promoting healing of a diabetic wound, and a preparation method and use thereof. In the preparation method of the autophagosome provided in the present disclosure, the autophagosome is extracted from a starved vascular endothelial cell, and the production of the autophagosome does not require induction by an additional reagent. The preparation method of the present disclosure involves simple extraction conditions, does not require an ultracentrifuge, and can be implemented in an ordinary laboratory. In addition, a yield of the autophagosome is higher than a yield of an exosome. The present disclosure provides an easy-to-implement and low-cost treatment method without toxic and side effects for a patient suffering from a chronic refractory wound. The autophagosome of the present disclosure can promote healing of a diabetic wound, and reduce physiological and psychological burdens caused by a diabetic wound to a patient.

Abstract: The present disclosure belongs to the technical fields of genetic engineering and biotherapeutic drugs, and relates to an engineered exosome for treating hypertrophic scar (HTS), and a preparation method and use thereof. In the present disclosure, the engineered exosome for treating HTS is a mesenchymal stem cell (MSC)-derived exosome overexpressing miR-141-3p. The exosome can significantly reduce formation of the HTS.

Abstract: The present disclosure provides a circCDK13-enriched engineered small extracellular vesicle (E-sEV), and a preparation method and use thereof, and belongs to the technical field of biomedicine. The present disclosure provides a preparation method of the circCDK13-enriched E-sEV. In the present disclosure, human placenta-derived mesenchymal stem cells (hP-MSCs) are infected with a vector overexpressing circCDK13, and a resulting cell supernatant is collected to obtain a small extracellular vesicle (sEV) overexpressing the circCDK13, which is used for wound healing in diabetes mellitus (DM). The E-sEV shows a therapeutic effect on DM wounds that is significantly better than that of natural small extracellular vesicles (N-sEVs) secreted by the hP-MSCs. This product not only exhibits advantages in healing speed, but also has a greater application potential in stimulating skin appendage regeneration and improving the quality of wound healing.

Abstract: The present disclosure provides a photocurable hydrogel loaded with VH298-modified exosome and a method of preparation and use thereof, belonging to the technical field of medical materials. In the present disclosure, an engineered exosome (VH-EVs) is prepared by combining an exosome with VH298, a hypoxia-inducible factor 1 alpha (HIF-1?) stabilizer; and a solid auxiliary material is loaded by a GelMA hydrogel. The material is not only conducive to sustained release of the engineered exosome, but improves angiogenesis and accelerate wound healing, showing a relatively high value for use.

Abstract: The present disclosure provides a photocurable hydrogel loaded with VH298-modified exosome and a method of preparation and use thereof, belonging to the technical field of medical materials. In the present disclosure, an engineered exosome (VH-EVs) is prepared by combining an exosome with VH298, a hypoxia-inducible factor 1 alpha (HIF-1?) stabilizer; and a solid auxiliary material is loaded by a GelMAhydrogel. The material is not only conducive to sustained release of the engineered exosome, but improves angiogenesis and accelerate wound healing, showing a relatively high value for use.

Abstract: The invention discloses a method for constructing functional exosomes capable of efficiently loading specific miRNA. In order to enable the exosome to carry miRNA with specific regulation function more efficiently so as to play a role in targeted regulation more accurately and efficiently, MS2 phage capsid protein is utilized to edit and construct a capture element of a specific miRNA molecule, and placenta mesenchymal stem cells are reprogrammed to enable the secreted exosome to efficiently load a target miRNA molecule, so that the target miRNA molecule is delivered to tissue cells to play a role in effective regulation, and therefore a new strategy is provided for realizing specific precise treatment in the future.

Abstract: The invention discloses a method for constructing functional exosomes capable of efficiently loading specific miRNA. In order to enable the exosome to carry miRNA with specific regulation function more efficiently so as to play a role in targeted regulation more accurately and efficiently, MS2 phage capsid protein is utilized to edit and construct a capture element of a specific miRNA molecule, and placenta mesenchymal stem cells are reprogrammed to enable the secreted exosome to efficiently load a target miRNA molecule, so that the target miRNA molecule is delivered to tissue cells to play a role in effective regulation, and therefore a new strategy is provided for realizing specific precise treatment in the future.

Abstract: Embodiments of the present disclosure disclose a method, a terminal, and a system for communication pairing of a digital television terminal and a mobile terminal. The method for communication pairing with a digital television terminal includes: acquiring communication connection information of the digital television terminal, wherein the communication connection information includes communication hardware information of the digital television terminal; performing encoding according to the communication connection information to generate a two-dimensional code image; displaying the two-dimensional code image; establishing a link connection to a mobile terminal that acquires the two-dimensional code image by using a camera; and performing communication pairing with the mobile terminal by means of the established link connection. By using the present disclosure, communication connection and pairing between a mobile terminal and a digital television terminal can be completed easily and conveniently.

Abstract: Embodiments of the present disclosure disclose a method, a terminal, and a system for communication pairing of a digital television terminal and a mobile terminal. The method for communication pairing with a digital television terminal includes: acquiring communication connection information of the digital television terminal, wherein the communication connection information includes communication hardware information of the digital television terminal; performing encoding according to the communication connection information to generate a two-dimensional code image; displaying the two-dimensional code image; establishing a link connection to a mobile terminal that acquires the two-dimensional code image by using a camera; and performing communication pairing with the mobile terminal by means of the established link connection. By using the present disclosure, communication connection and pairing between a mobile terminal and a digital television terminal can be completed easily and conveniently.

Abstract: Embodiments of the present disclosure disclose a method, a terminal, and a system for communication pairing of a digital television terminal and a mobile terminal. The method for communication pairing with a digital television terminal includes: acquiring communication connection information of the digital television terminal, wherein the communication connection information includes communication hardware information of the digital television terminal; performing encoding according to the communication connection information to generate a two-dimensional code image; displaying the two-dimensional code image; establishing a link connection to a mobile terminal that acquires the two-dimensional code image by using a camera; and performing communication pairing with the mobile terminal by means of the established link connection. By using the present disclosure, communication connection and pairing between a mobile terminal and a digital television terminal can be completed easily and conveniently.

Abstract: A method for switching application program in a touch screen terminal, comprising: in a running interface of an application program, displaying an application program switching window in a screen sweet area upon a condition that a lateral slide signal is detected which is triggered at a screen vertical edge of the screen sweet area and laterally slid to an opposite side of the screen vertical edge; upon a condition that the application program switching window is being displayed, quitting the application program switching window from the screen sweet area in the event that a lateral slide signal is detected which is triggered at an arbitrary point in the touch screen and laterally slid to the screen vertical edge of the screen sweet area; and switching to a corresponding application program in the event that a click signal to an application program icon within the application program switching window is detected.