Abstract: A checking mechanism for workshop security applying a method for workshop security comprises a transmission device, a recording device, a storage subassembly and a control subassembly. The transmission device comprises a first end (115) and a second end (116). The recording device records personnel and objects in their possession through a security check process, to establish a correspondence. The storage subassembly stores the information comprising personnel and their objects. The control subassembly is used for controlling the recording device to record and store the information to the storage subassembly. The control subassembly can retrieve the information recorded by the recording device to detect a certain person's correspondence to the certain object.

Abstract: Provided herein are methods of assaying a methylated DNA-binding protein comprising contacting methylated DNA-binding proteins with labeled oligonucleotides, obtaining sample partitions for oligonucleotides with different methylation states, and quantifying the labeled oligonucleotides in the partitions.

Abstract: A checking mechanism for workshop security applying a method for workshop security comprises a transmission device, a recording device, a storage subassembly and a control subassembly. The transmission device comprises a first end (115) and a second end (116). The recording device records personnel and objects in their possession through a security check process, to establish a correspondence. The storage subassembly stores the information comprising personnel and their objects. The control subassembly is used for controlling the recording device to record and store the information to the storage subassembly. The control subassembly can retrieve the information recorded by the recording device to detect a certain person’s correspondence to the certain object.

Abstract: A sleeve wrapping device includes a first fixing base, a second fixing base, a first supporting frame protruded from the first fixing base, a second supporting frame protruded from the second fixing base, a first roller mounted on the first supporting frame, a second roller mounted on the second supporting frame, and a sleeve wrapping film comprising a substrate and a plurality of adhesive layers. The first roller and the second roller move back and forth relative to the first supporting frame and the second supporting frame. The sleeve wrapping device is switched between a first state that the first roller and the second roller are in contact with each other and a second state that the first roller and the second roller are separated. The substrate winds the first roller and the second roller.

Abstract: A sleeve wrapping device includes a first fixing base, a second fixing base, a first supporting frame protruded from the first fixing base, a second supporting frame protruded from the second fixing base, a first roller mounted on the first supporting frame, a second roller mounted on the second supporting frame, and a sleeve wrapping film comprising a substrate and a plurality of adhesive layers. The first roller and the second roller move back and forth relative to the first supporting frame and the second supporting frame. The sleeve wrapping device is switched between a first state that the first roller and the second roller are in contact with each other and a second state that the first roller and the second roller are separated. The substrate winds the first roller and the second roller.

Abstract: A lithium ion battery includes an anode plate and a cathode plate spirally coiled with a separator set between the anode plate and the cathode plate. The cathode plate includes a cathode current collector and a cathode film on the cathode current collector. The cathode current collector is formed with a number of cathode extending portions along a length direction thereof. The anode plate includes an anode current collector and an anode film on the anode current collector. The anode current collector is formed with a number of anode extending portions along a length direction thereof. After the cathode plate, the anode plate and the separator are coiled as a battery cell, the cathode extending portions are combined to form a cathode lead, and the anode extending portions are combined to form an anode lead. Before manufacture, the compacted cathode plate/anode plate is baked.

Abstract: Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry.

Abstract: Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry.

Abstract: Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry.

Abstract: Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a source of magnesium ion, and a suitable DNA substrate; measuring the activity level of mammalian Mus81-Eme endonuclease complex in the mixture; comparing the measured activity level to the activity level of a substantially similar mixture of isolated Mus81-Eme1 endonuclease, magnesium ion, and DNA substrate in the absence of the chemical compound to be tested; and selecting a chemical compound that increases or decreases the endonuclease activity. Isolated mammalian Eme1 and Eme2 proteins derived from humans and murine species and isolated nucleic acids encoding the proteins are also described.

Abstract: Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a source of magnesium ion, and a suitable DNA substrate; measuring the activity level of mammalian Mus81-Eme endonuclease complex in the mixture; comparing the measured activity level to the activity level of a substantially similar mixture of isolated Mus81-Eme1 endonuclease, magnesium ion, and DNA substrate in the absence of the chemical compound to be tested; and selecting a chemical compound that increases or decreases the endonuclease activity. Isolated mammalian Eme1 and Eme2 proteins derived from humans and murine species and isolated nucleic acids encoding the proteins are also described.