Abstract: This invention discloses a novel system and method for expressing multiple gene products in oleaginous yeasts including Yarrowia lipolytica and Rhodotorula toruloides. More particularly, the present disclosure provides novel promoters functional in Y. lipolytica which can be used for producing a broad range of bioproducts.

Abstract: The present disclosure provides a method for genetically engineering Yarrowia lipolytica host cell for producing glycolic acid from organic wastes. A subject genetically engineered Y. lipolytica cell comprises the disrupted native genes encoding malate synthase, heterologous enzyme of glyoxylate reductase targeted in the different cellular compartments including mitochondria, peroxisome and cytosol, and a mutant NADP+-dependent malate dehydrogenase. The pathway with a theoretical yield as high as that 1 g of acetic acid can be converted to 1.27 g of glycolic acid without carbon loss was engineered for glycolic acid production. The methods particularly include process for production of volatile fatty acids (VFAs) mainly comprised of acetic acid from organic waste, and then use of resultant VFAs for biosynthesis of glycolic acid by recombinant Y. lipolytica.

Abstract: An alternative biochemical process for fuels and chemicals production from cellulosic biomass is presented. The method includes an aerobic step where microorganisms convert carbohydrates contained in cellulosic biomass into sugar acids, which can be converted to fuels and chemicals, including ethanol, gluconate, and acetic acid, via fermentation.