Patents by Inventor Xiaochuan Zhou
Xiaochuan Zhou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130261026Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: ApplicationFiled: June 10, 2013Publication date: October 3, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Xiaolian Gao, Hua Zhang, Peillin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Patent number: 8461317Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: GrantFiled: October 11, 2011Date of Patent: June 11, 2013Assignee: The Regents of the University of MichiganInventors: Xiaolian Gao, Hua Zhang, Peillin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Publication number: 20120071358Abstract: The present invention describes microfluidic devices that provide novel fluidic structures to facilitate the separation of fluids into isolated, pico-liter sized compartments for performing multiplexing chemical and biological reactions. Applications of the novel devices including biomolecule synthesis, polynucleotide amplification, and binding assays are also disclosed.Type: ApplicationFiled: June 6, 2011Publication date: March 22, 2012Inventors: Xiaochuan Zhou, Xiaolian Gao
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Publication number: 20120029166Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: ApplicationFiled: October 11, 2011Publication date: February 2, 2012Applicant: THE REGENTS OF THE UNVERSITY OF MICHIGANInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Patent number: 8053187Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: GrantFiled: October 4, 2010Date of Patent: November 8, 2011Assignee: The Regents of the University of MichiganInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Publication number: 20110251109Abstract: Fluidic methods and devices for conducting parallel chemical reactions are disclosed. The methods are based on the use of in situ photogenerated reagents such as photogenerated acids, photogenerated bases, or any other suitable chemical compounds that produce active reagents upon light radiation. The present invention describes devices and methods for performing a large number of parallel chemical reactions without the use of a large number of valves, pumps, and other complicated fluidic components. The present invention provides microfluidic devices that contain a plurality of microscopic vessels for carrying out discrete chemical reactions.Type: ApplicationFiled: October 1, 2009Publication date: October 13, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiaochuan Zhou, Tiecheng Zhou, David Sun
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Publication number: 20110170075Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.Type: ApplicationFiled: November 19, 2010Publication date: July 14, 2011Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
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Publication number: 20110097762Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: ApplicationFiled: October 4, 2010Publication date: April 28, 2011Applicant: THE REGENTS OF THE UNVERSITY OF MICHIGANInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Publication number: 20110008775Abstract: The present invention relates to the field of analysis of nucleic acid sequences. More specifically, the present invention relates to the method and instrument for high throughput parallel DNA sequencing. The present invention also provides method for selection of sequences from analyte samples for enrichment of the target sequences or depletion of the selected molecules and in particular undesirable sequence templates from sequencing samples.Type: ApplicationFiled: December 10, 2008Publication date: January 13, 2011Inventors: Xiaolian Gao, Xiaochuan Zhou
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Patent number: 7838466Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.Type: GrantFiled: May 29, 2002Date of Patent: November 23, 2010Assignees: The Regents of the University of Michigan, University of HoustonInventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
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Patent number: 7807807Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: GrantFiled: June 29, 2009Date of Patent: October 5, 2010Assignee: The Regents of the University of MichiganInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Publication number: 20100112558Abstract: The present invention relates to the field of methods and devices of miniaturized synthesis. More specifically, the present invention relates to the parallel synthesis of large number of different types of molecules and oligomers, such as oligonucleotides (oligos), peptides, lipids, carbohydrates, small ligand molecules, and other organic and inorganic molecules as probes for multiplexing assays. The probes may be synthesized from and/or attached to nanobeads to microbeads. The present invention provides for assays of multiplexing large scale biology, such as analysis of genomic DNAs and RNAs and proteomic proteins or peptides performed simultaneously on the synthetic beads.Type: ApplicationFiled: November 3, 2008Publication date: May 6, 2010Inventors: Xiaolian Gao, Xiaochuan Zhou
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Publication number: 20100093974Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: ApplicationFiled: June 29, 2009Publication date: April 15, 2010Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Publication number: 20100047875Abstract: The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.Type: ApplicationFiled: April 7, 2009Publication date: February 25, 2010Inventors: Xiaioian Gao, Xiaochuan Zhou, Xiaolin Zheng, Niling Sheng, Qi Zhu
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Patent number: 7553958Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: GrantFiled: March 21, 2007Date of Patent: June 30, 2009Assignee: The Regents of the University of MichiganInventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
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Patent number: 7544638Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for generating a photogenerated reaction in solution on a substrate comprising generating a light beam using an optical device system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device and using the spatial optical modulator to direct light to fluidly isolated reaction sites under conditions such that a photogenerated reaction takes place. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.Type: GrantFiled: January 13, 2003Date of Patent: June 9, 2009Assignees: The Regents of the University of Michigan, University of HoustonInventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
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Patent number: 7544793Abstract: The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.Type: GrantFiled: March 1, 2006Date of Patent: June 9, 2009Inventors: Xialoian Gao, Xiaochuan Zhou, Xiaolin Zhang, Nuing Sheng, Qi Zhu
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Patent number: 7491680Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In some embodiments, the present invention provides methods and devices for generating one or more selected multimers at specific reaction sites on a substrate comprising contacting the substrate with a liquid solution comprising photo-reagent precursors, isolating the reaction sites, selectively irradiating the reaction sites with a digital micromirror device, and contacting the substrate with monomers. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.Type: GrantFiled: September 11, 2002Date of Patent: February 17, 2009Assignees: The Regents of the University of Michigan, University of HoustonInventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
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Publication number: 20080318806Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for generating a photogenerated reaction in solution on a substrate comprising generating a light beam using an optical device system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device and using the spatial optical modulator to direct light to fluidly isolated reaction sites under conditions such that a photogenerated reaction takes place. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.Type: ApplicationFiled: January 13, 2003Publication date: December 25, 2008Applicant: The Regents Of The University Of MichiganInventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
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Publication number: 20070287832Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.Type: ApplicationFiled: March 21, 2007Publication date: December 13, 2007Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Pellois, Qin Xiang, Xiaochuan Zhou