Patents by Inventor Xiaochun Fan
Xiaochun Fan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240095935Abstract: Methods and systems for deep learning alignment for semiconductor applications are provided. One method includes transforming design information for an alignment target on a specimen to a predicted image of the alignment target by inputting the design information into a deep learning model and aligning the predicted image to an image of the alignment target on the specimen generated by an imaging subsystem. The method also includes determining an offset between the predicted image and the image generated by the imaging subsystem based on results of the aligning and storing the determined offset as an align-to-design offset for use in a process performed on the specimen with the imaging subsystem.Type: ApplicationFiled: March 5, 2023Publication date: March 21, 2024Inventors: Hong Chen, Ziqi Fan, Richard Wallingford, Xiaochun Li, Sangbong Park
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Publication number: 20220073954Abstract: Described are compositions and methods related to hybrid yeast that produces an increased amount of ethanol from starch-containing substrates compared to its parental yeast. Such yeast is well-suited for use in fuel alcohol production to increase yield.Type: ApplicationFiled: January 7, 2020Publication date: March 10, 2022Inventors: Xiaochun FAN, Kerry HOLLANDS, Barbara Urszula KOZAK, Elizabeth A. KRASLEY, Celia Emily Gaby PAYEN, Min QI
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Publication number: 20210179674Abstract: Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.Type: ApplicationFiled: October 22, 2018Publication date: June 17, 2021Inventors: Xiaochun Fan, Zhongqiang Chen, Min Qi
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Publication number: 20200263165Abstract: Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a Pol-II promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a eukaryote cell. The present disclosure further describes methods and compositions employing said recombinant DNA construct to modify the genome of non-conventional yeast.Type: ApplicationFiled: December 15, 2016Publication date: August 20, 2020Inventors: Felipe Oseas Bendezu, Xiaochun Fan, Ryan L. Frisch, Seung-Pyo Hong
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Publication number: 20200190540Abstract: Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5?-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5?-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.Type: ApplicationFiled: October 30, 2019Publication date: June 18, 2020Inventors: Ryan Frisch, Xiaochun Fan, Seung-Pyo Hong
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Publication number: 20200157516Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.Type: ApplicationFiled: January 27, 2020Publication date: May 21, 2020Inventors: RYAN L. FRISCH, XIAOCHUN FAN, SEUNG-PYO HONG, ETHEL NOLAND JACKSON
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Patent number: 10626424Abstract: Recombinant microbial cells comprising an engineered LCDA production pathway that comprises at least one up-regulated long-chain acyl-CoA synthetase (ACoS) are disclosed. These recombinant microbial cells are capable of producing one or more long-chain dicarboxylic acid (LCDA) products from a long-chain fatty acid-comprising substrate. Methods of using recombinant microbial cells to produce LCDAs are also disclosed.Type: GrantFiled: July 20, 2016Date of Patent: April 21, 2020Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLCInventors: Quinn Qun Zhu, Xiaochun Fan, Seung-Pyo Hong, Despina Boughioukou, Dongming Xie, Himanshu H. Dhamankar
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Patent number: 10584322Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.Type: GrantFiled: December 13, 2018Date of Patent: March 10, 2020Assignee: DUPONT US HOLDING, LLCInventors: Ryan L Frisch, Xiaochun Fan, Seung-Pyo Hong, Ethel Noland Jackson
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Patent number: 10513711Abstract: Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5?-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5?-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.Type: GrantFiled: July 5, 2015Date of Patent: December 24, 2019Assignee: DUPONT US HOLDING, LLCInventors: Ryan Frisch, Xiaochun Fan, Seung-Pyo Hong
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Publication number: 20190300877Abstract: Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a tRNA promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said recombinant DNA construct does not comprise a nucleotide sequence encoding a ribozyme, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a microbial cell.Type: ApplicationFiled: April 9, 2019Publication date: October 3, 2019Inventors: FELIPE OSEAS BENDEZU, XIAOCHUN FAN, RYAN L. FRISCH, SEUNG-PYO HONG
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Publication number: 20190144897Abstract: Recombinant microbial cells comprising an engineered LCDA production pathway that comprises at least one up-regulated long-chain acyl-CoA synthetase (ACoS) are disclosed. These recombinant microbial cells are capable of producing one or more long-chain dicarboxylic acid (LCDA) products from a long-chain fatty acid-comprising substrate. Methods of using recombinant microbial cells to produce LCDAs are also disclosed.Type: ApplicationFiled: July 20, 2016Publication date: May 16, 2019Inventors: Quinn Qun Zhu, Xiaochun Fan, Seung-Pyo Hong, Despina Boughioukou, Dongming Xie, Himanshu H. Dhamankar
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Publication number: 20190100737Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.Type: ApplicationFiled: December 13, 2018Publication date: April 4, 2019Inventors: RYAN L. FRISCH, XIAOCHUN FAN, SEUNG-PYO HONG, ETHEL NOLAND JACKSON
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Patent number: 10208298Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.Type: GrantFiled: November 3, 2015Date of Patent: February 19, 2019Assignee: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Ryan L. Frisch, Xiaochun Fan, Seung-Pyo Hong, Ethel Noland Jackson
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Publication number: 20170335300Abstract: A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.Type: ApplicationFiled: November 3, 2015Publication date: November 23, 2017Inventors: Ryan L. Frisch, Xiaochun Fan, Seung-Pyo Hong, Ethel Noland Jackson
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Publication number: 20170226533Abstract: Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5?-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5?-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.Type: ApplicationFiled: July 5, 2015Publication date: August 10, 2017Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Ryan FRISCH, Xiaochun FAN, Seung-Pyo HONG,
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Patent number: 9701989Abstract: Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: GrantFiled: December 18, 2013Date of Patent: July 11, 2017Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Pamela L Sharpe, Narendra S Yadav, Xiaochun Fan, Michael Dauner
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Publication number: 20140186906Abstract: Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: ApplicationFiled: December 18, 2013Publication date: July 3, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Pamela L. Sharpe, Narendra S. Yadav, Xiaochun Fan, Michael Dauner