Abstract: A preparation method for a quartz reference sample for calibration of a luminescence dating instrument includes the following steps: preparing a quartz glass front wall, a quartz glass rear wall and an annular gasket; placing the annular gasket between the quartz glass front wall and the quartz glass rear wall, and placing a quartz sample in a center hole of the annular gasket; wrapping the loading device with aluminum foil, placing the loading device on a dedicated stand and positioning the dedicated stand at a measuring point of a reference radiation field; measuring an air kerma rate at the measuring point; calculating a conversion coefficient from air kerma to an absorbed dose of quartz; calculating an absorbed dose of the quartz sample; assessing the homogeneity of the quartz sample; and assessing the stability of the absorbed dose of quartz.