Abstract: Systems, methods, and computer program products for fugitive emission determinations are provided. An example imaging system includes a first infrared (IR) imaging device configured to generate first IR image data of a field of view of the first IR imaging device that include one or more data entries associated with a fugitive emission from an emission source. The system further includes a computing device operably connected with the first IR imaging device and configured to receive the first IR image data from the first IR imaging device, generate spectral absorption data based on the first IR image data, and determine a gas amount associated with the fugitive emission based upon the spectral absorption data. The computing device also determines a leak rate and leak duration of the fugitive emission based upon the determined gas amount and determines a total emission loss based on the same.

Abstract: The present invention relates to the technical field of nucleic acids, and in particular, to a method for precisely preparing a circular RNA with an Anabaena intron self-cleaving ribozyme. By redesigning a target nucleic acid sequence of a linear RNA, selecting a site suitable for precise cleavage by the intron self-cleaving ribozyme, and removing exon sequences from the Anabaena intron self-cleaving ribozyme, precise cyclization of RNAs after in vitro transcription is achieved. The method provided by the present invention solves the problem in the prior art that linear RNAs cannot be precisely cyclized during the process of generating circular RNAs in vitro, and provides an ideal in vitro preparation method for circular RNA applications.

Abstract: Systems, methods, and computer program products for fugitive emission determinations are provided. An example imaging system includes a first infrared (IR) imaging device configured to generate first IR image data of a field of view of the first IR imaging device that include one or more data entries associated with a fugitive emission from an emission source. The system further includes a computing device operably connected with the first IR imaging device and configured to receive the first IR image data from the first IR imaging device, generate spectral absorption data based on the first IR image data, and determine a gas amount associated with the fugitive emission based upon the spectral absorption data. The computing device also determines a leak rate and leak duration of the fugitive emission based upon the determined gas amount and determines a total emission loss based on the same.

Abstract: The present invention relates to the technical field of nucleic acids, and in particular, to a method for precisely preparing a circular RNA with an Anabaena intron self-cleaving ribozyme. By redesigning a target nucleic acid sequence of a linear RNA, selecting a site suitable for precise cleavage by the intron self-cleaving ribozyme, and removing exon sequences from the Anabaena intron self-cleaving ribozyme, precise cyclization of RNAs after in vitro transcription is achieved. The method provided by the present invention solves the problem in the prior art that linear RNAs cannot be precisely cyclized during the process of generating circular RNAs in vitro, and provides an ideal in vitro preparation method for circular RNA applications.

Abstract: Systems, methods, and computer program products for fugitive emission determinations are provided. An example imaging system includes a first infrared (IR) imaging device configured to generate first IR image data of a field of view of the first IR imaging device that include one or more data entries associated with a fugitive emission from an emission source. The system further includes a computing device operably connected with the first IR imaging device and configured to receive the first IR image data from the first IR imaging device, generate spectral absorption data based on the first IR image data, and determine a gas amount associated with the fugitive emission based upon the spectral absorption data. The computing device also determines a leak rate and leak duration of the fugitive emission based upon the determined gas amount and determines a total emission loss based on the same.