Patents by Inventor Xiaodan SHEN

Xiaodan SHEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of expressing goldfish gfTP1 tranposase protein
    Patent number: 9944953
    Abstract: An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1 (DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.
    Type: Grant
    Filed: January 4, 2015
    Date of Patent: April 17, 2018
    Assignee: Shanghai Ocean University
    Inventors: Shuming Zou, Xiayun Jiang, Haili Xu, Fei Hou, Luodan Cheng, Xiaodan Shen, Shouchang Deng
  • EXPRESSION METHOD OF ACTIVE GOLDFISH TGF2 TRANSPOSON RECOMBINANT TRANSPOSASE PROTEIN
    Publication number: 20160215299
    Abstract: An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1(DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.
    Type: Application
    Filed: January 4, 2015
    Publication date: July 28, 2016
    Inventors: Shuming ZOU, Xiayun JIANG, Haili XU, Fei HOU, Luodan CHENG, Xiaodan SHEN, Shouchang DENG