Abstract: Provided is a liquid sample sampling device used in an operation, and belongs to the technical field of sampling devices. The sampling device includes a negative pressure suction head, an auxiliary tube connected with a side wall of the negative pressure suction head, and a sampling bottle connected with the auxiliary tube. A rebound connecting plate is arranged at a position connecting the negative suction head and the auxiliary tube. The side wall of the negative pressure suction head is provided with a push switch. A diameter of the auxiliary tube is larger than a diameter of the negative pressure suction head. The push switch is an elastic membrane switch.

Abstract: The invention discloses a method for the detection of sialate glycosyl casein glycomacropeptide by boronate affinity column enrichment-liquid chromatography-tandem mass spectrometry using phenylboric acid modified mesoporous silica as packing material, which belongs to the field of food analysis and detection. The method includes the following steps: (1) sample preparation; (2) enrichment and purification of boronate affinity column; (3) liquid chromatography-tandem mass spectrometry detection. The invention makes use of the affinity property of phenylboric acid to the special sugar group sialic acid on the serine and threonine residues in casein glycogiant peptide, regulates the adsorption and elution of casein glycogiant peptide with sialic acid group by changing pH.

Abstract: The present disclosure discloses use of TM9SF1 gene as a target in vascular diseases, which relates to the field of biotechnology. In the present disclosure, by means of RNA interference strategy, it is found that after endogenous TM9SF1 gene is interfered with, the expression of two important genes IL1? and IL8 related to the inflammation of HUVEC and the expression of the gene ACE1 closely related to vasoconstriction are remarkably downregulated, suggesting that TM9SF1 gene has positive regulation effect on the expression of IL1?, IL8 and ACE1 genes. By inhibiting or silencing the expression of the TM9SF1 gene, it is possible to inhibit or silence the expression of IL1?, IL8, and ACE1 genes, and further achieve the object of treating or preventing vascular diseases associated with the expression level of IL1?, IL8 and ACE1 genes.