Abstract: The present invention discloses a serum-free medium for full suspension culture of MDCK cells and a preparation method of the serum-free medium. The serum-free medium for full suspension culture of the MDCK cells comprises basic metabolic nutrients, nucleotide, vitamins, inorganic salts, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives. The preparation method of the serum-free medium for the full suspension culture of the MDCK cells comprises the following steps: 1) preparing a mixed solution: dissolving and mixing raw materials; and 2) regulating pH: regulating the pH of the mixed solution to 6.3 to 6.7, and setting a constant volume to obtain the serum-free medium for the full suspension culture of the MDCK cells.

Abstract: A device and a method are provided to automatically generate test case for embedded software. This invention is in software test field, including symbolic execution kernel module, path selection module, solver, debugger, concrete execution kernel module and debugger agent module. The tested software and test cases are uploaded from the host system to the embedded system through debugger and debugger agent. The concrete execution kernel module starts the tested software. The symbolic execution kernel module captures the run-time information of the tested software through the debugger. When the tested software operates on the symbol source, the symbolic execution kernel module marks the symbol source, tracks the symbol propagation, generates path condition and sends the path condition to path selection module. This invention can automatically generate test cases for embedded software, which doesn't need the source code of the tested software and can be conveniently used for commercial software.