Abstract: A hybridization method of Sinocyclocheilus grahami and carp, which comprises parent fish cultivation, artificial insemination, fish egg incubating and fry feeding, strictly controls environmental parameters and feed types of parent fish cultivation, simulates good living conditions for S. grahami and carp, is beneficial for wild S. grahami and carp to adapt to the artificial breeding environment quickly, realizes natural spermatogenesis and oviposition in artificial environment without using the oxytocin, and provides sperm and eggs both in quality and quantity for artificial hybridization. At the same time, the key parameters during incubation (including incubating water level, water temperature, water flow velocity, pH, dissolved oxygen and sterilization of fertilized eggs, etc.) are also defined to provide suitable environmental conditions for the successful incubation of fertilized eggs.

Abstract: A hybridization method of Sinocyclocheilus grahami and carp, which comprises parent fish cultivation, artificial insemination, fish egg incubating and fry feeding, strictly controls environmental parameters and feed types of parent fish cultivation, simulates good living conditions for S. grahami and carp, is beneficial for wild S. grahami and carp to adapt to the artificial breeding environment quickly, realizes natural spermatogenesis and oviposition in artificial environment without using the oxytocin, and provides sperm and eggs both in quality and quantity for artificial hybridization. At the same time, the key parameters during incubation (including incubating water level, water temperature, water flow velocity, pH, dissolved oxygen and sterilization of fertilized eggs, etc.) are also defined to provide suitable environmental conditions for the successful incubation of fertilized eggs.

Abstract: An artificial propagation method of Sinocyclocheilus rhinocerous, comprising placing female and male parent fish in a sterilized culture container in which a black cave simulant is placed in advance, strictly controlling the environmental parameters of the parent fish culture. The method realizes natural spawning and sperm production of S. rhinocerous under artificial conditions without using oxytocin. There is no oxytocin used in the artificial propagation, thus not only obtaining a higher fertilization rate and incubating rate, but also reducing the aquaculture cost. The operation is simpler and faster. At the same time, different feeds are fed in different growth stages according to the different growth characteristics of each stage of S. rhinocerous fry, thereby increasing the survival rate and reducing the deformity rate of fries. Therefore, the method provided by the present disclosure realizes efficient artificial propagation method of S. rhinocerous.