Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Provided are a tumor immune enhancer, a pharmaceutical composition thereof, and a preparation method therefor. The enhancer comprises one or more polypeptides using sequences shown in SEQ ID NOs.: 1-9 as core structures, and can be used for preparing tumor vaccines.

Abstract: The invention provides use of NW_006883358.1 in a CHO cell genome for stable expression of a protein. A site for stable expression of a protein in the CHO cell genome is located at the 6874389th base of CHO cell gene NW_006883358.1; 5?NNNNNNNNNNNNNNNNNNNNNGG3? within a range of 6874325-6874443 near the site, which can be identified by CRISPR/Cas9 technology, is a target sequence. In the present application, different protein genes are introduced to a fixed position in the CHO cell genome for stable expression.

Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Use of genomic NW_006880285.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 1235357 in a CHO cell gene NW_006880285.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 1235284-1235429 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: Agglomerated fine solids, e.g., agglomerated pellets (2B), that are located within a restricted space or pinch point of a conveyance system (1), and that impede the free-flow of fine solids from one vessel (4) to another vessel (10), are detected and fragmented through the coordinated use of a flow detector (12), breaker plate (8) and press (6). The flow detector (12) senses an interruption or diminishment in the flow of the fine solids and signals and actuates the press (6) which in turn engages and fragments the agglomeration. The breaker plate (8) is sized and configured to trap major agglomerates.

Abstract: Agglomerated fine solids, e.g., agglomerated pellets (2B), that are located within a restricted space or pinch point of a conveyance system (1), and that impede the free-flow of fine solids from one vessel (4) to another vessel (10), are detected and fragmented through the coordinated use of a flow detector (12), breaker plate (8) and press (6). The flow detector (12) senses an interruption or diminishment in the flow of the fine solids and signals and actuates the press (6) which in turn engages and fragments the agglomeration. The breaker plate (8) is sized and configured to trap major agglomerates.