Patents by Inventor Xiaohai Liu

Xiaohai Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250146066
    Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Application
    Filed: January 13, 2025
    Publication date: May 8, 2025
    Inventors: Antoine FRANCAIS, Elena CRESSINA, Adam CULLEY, Angelica MARIANI, Xiaolin WU, Xiaohai LIU
  • Patent number: 12286453
    Abstract: Embodiments of the present disclosure relate to nucleotide with acetal 3?-OH blocking groups. Also provided herein are methods of using fully functionalized nucleotides containing the 3? acetal blocking group for sequencing applications.
    Type: Grant
    Filed: September 28, 2023
    Date of Patent: April 29, 2025
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Elena Cressina, Angelica Mariani, Adam Culley, Anno Koetje, Xiaohai Liu
  • Patent number: 12227802
    Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Grant
    Filed: October 17, 2023
    Date of Patent: February 18, 2025
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Patent number: 12195796
    Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.
    Type: Grant
    Filed: May 2, 2023
    Date of Patent: January 14, 2025
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu, Nikolai Nikolaevich Romanov, Antoine Francais, Patrick McCauley, Michael Callingham, Carole Anastasi
  • Publication number: 20240400605
    Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3? phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
    Type: Application
    Filed: August 15, 2024
    Publication date: December 5, 2024
    Inventors: Xiaolin Wu, Randall Smith, Peyton Shieh, John M. Beierle, Wayne N. George, Elliot John Lawrence, Jie Mao, Xiaohai Liu
  • Publication number: 20240352048
    Abstract: The present application relates to substituted dyes containing bis-boron fused heterocycles and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: July 1, 2024
    Publication date: October 24, 2024
    Inventors: Michael Callingham, Nikolai Nikolaevich Romanov, Xiaohai Liu, Justyna Piekos
  • Patent number: 12084474
    Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3? phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: September 10, 2024
    Assignee: Illumina, Inc.
    Inventors: Xiaolin Wu, Randall Smith, Peyton Shieh, John M. Beierle, Wayne N. George, Elliot John Lawrence, Jie Mao, Xiaohai Liu
  • Publication number: 20240294967
    Abstract: Embodiments of the present disclosure relates to various bisulfite-free chemical methods for detecting methylation of cytosine in the DNA sample. These methods convert methylated and hydroxymethylated cytosine in the nucleic acid sequence to a modified or pseudo thymine or a uracil moiety which then can be detected in sequencing.
    Type: Application
    Filed: January 18, 2023
    Publication date: September 5, 2024
    Inventors: Xiaolin WU, Antoine FRANCAIS, Xiaohai LIU
  • Patent number: 12071662
    Abstract: The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.
    Type: Grant
    Filed: May 5, 2023
    Date of Patent: August 27, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Elena Cressina, Antoine Francais, Xiaohai Liu
  • Publication number: 20240271206
    Abstract: Embodiments of the present disclosure relate to nucleotides with 3? allyl blocking groups. Also provided herein are methods of sequencing using nucleotides with 3? allyl blocking groups described herein, and sequencing kits.
    Type: Application
    Filed: December 21, 2023
    Publication date: August 15, 2024
    Inventors: Antoine Francais, Elena Cressina, Kathryn Hattingh, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240271209
    Abstract: The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: April 9, 2024
    Publication date: August 15, 2024
    Inventors: Nikolai Nikolaevich Romanov, Michael Callingham, Carole Anastasi, Patrick McCauley, Niall Hynes, Natasha Crake, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240271185
    Abstract: Disclosed herein include methods, compositions, reaction mixtures, kits and systems for identification of methylated cytosines in nucleic acids using a bisulfite-free, one-step chemoenzymatic modification of methylated cytosines.
    Type: Application
    Filed: August 16, 2022
    Publication date: August 15, 2024
    Inventors: Colin Brown, Xiaohai Liu, Xiaolin Wu, Eric Brustad, Sarah E. Shultzaberger
  • Patent number: 12043637
    Abstract: The present application relates to substituted dyes containing bis-boron fused heterocycles and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: May 4, 2022
    Date of Patent: July 23, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Michael Callingham, Nikolai Nikolaevich Romanov, Xiaohai Liu, Justyna Piekos
  • Patent number: 12031178
    Abstract: Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.
    Type: Grant
    Filed: February 14, 2023
    Date of Patent: July 9, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu, Nikolai Nikolaevich Romanov, Antoine Francais, Patrick McCauley, Michael Callingham, Carole Anastasi
  • Publication number: 20240218443
    Abstract: The present application relates to palladium compositions, methods for sequencing by synthesis using nucleotides with 3? blocking groups, and sequencing kits, where one or more palladium scavengers were used to improve sequencing metrics such phasing and prephasing values.
    Type: Application
    Filed: December 21, 2023
    Publication date: July 4, 2024
    Inventors: Angelica Mariani, Antoine Francais, Frederick James Topping, Christopher Winnard, Philip Balding, Chol Steven Yun, Patrizia Iavicoli, Kathryn Hattingh, Xiaolin Wu, Xiaohai Liu
  • Publication number: 20240209015
    Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal or allyl 3? blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3? blocking groups for sequencing applications.
    Type: Application
    Filed: December 21, 2023
    Publication date: June 27, 2024
    Inventors: Antoine Francais, Elena Cressina, Angelica Mariani, Adam Culley, Anno Koetje, Xiaohai Liu, Xiaolin Wu, Kathryn Hattingh
  • Publication number: 20240182963
    Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3? acetal, thiocarbamate or allyl blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Application
    Filed: December 21, 2023
    Publication date: June 6, 2024
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu, Kathryn Hattingh
  • Patent number: 11981964
    Abstract: The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: July 26, 2021
    Date of Patent: May 14, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Michael Callingham, Carole Anastasi, Patrick Mccauley, Niall Hynes, Natasha Crake, Xiaolin Wu, Xiaohai Liu
  • Patent number: 11981955
    Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: December 6, 2022
    Date of Patent: May 14, 2024
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Xiaohai Liu
  • Publication number: 20240150827
    Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.
    Type: Application
    Filed: October 17, 2023
    Publication date: May 9, 2024
    Inventors: Antoine FRANCAIS, Elena CRESSINA, Adam CULLEY, Angelica MARIANI, Xiaolin WU, Xiaohai LIU