Abstract: The present invention relates to the field of biotechnology, in particular to a DNA polymerase mutant and the use thereof. Compared with a wild type, the mutant provided in the present invention has a significantly improved DNA polymerization activity, significantly improved affinity to DNA and amplification uniformity; and has good effects in the amplification of multiplex PCR, high-GC templates and complex templates. A hot-start-version DNA polymerase is further prepared by means of using antibodies or chemical modifications, wherein the yield of the DNA polymerase is significantly improved, while the primer dimer is significantly decreased. The prepared mutant hot-start DNA polymerase can be applied to PCR amplification of multiplex PCR, high-GC templates and complex templates.

Abstract: The present disclosure relates to the technical field of optimization of charging facilities, and in particular, to a day-ahead coordinated optimization scheduling method for a truck mobile charging station.

Abstract: A bilevel coordinated optimization method for fixed and mobile charging facilities on highways, includes: constructing an optimization model framework which includes an upper-layer coordinated location optimization model and a lower-layer coordinated capacity optimization model, where the upper-layer coordinated location optimization model is used to optimize locations of charging stations and determine locations and time points of charging demands that require truck mobile charger (TMC) deployment, while the lower-layer coordinated capacity optimization model is used to optimize TMC and fixed charger (FC) capacities at candidate sites, improving an utilization rate of FCs; and performing equivalent linearization on a nonlinear problem using a big-M method and converting the problem into a mixed-integer linear programming model, and implementing a data exchange process between upper and lower layers using analytical target cascading.

Abstract: A bilevel coordinated optimization method for fixed and mobile charging facilities on highways, includes: constructing an optimization model framework which includes an upper-layer coordinated location optimization model and a lower-layer coordinated capacity optimization model, where the upper-layer coordinated location optimization model is used to optimize locations of charging stations and determine locations and time points of charging demands that require truck mobile charger (TMC) deployment, while the lower-layer coordinated capacity optimization model is used to optimize TMC and fixed charger (FC) capacities at candidate sites, improving an utilization rate of FCs; and performing equivalent linearization on a nonlinear problem using a big-M method and converting the problem into a mixed-integer linear programming model, and implementing a data exchange process between upper and lower layers using analytical target cascading.

Abstract: A method and apparatus for determining the service life of a bearing. By means of the load spectrum of electric motor output torques under various work conditions comprising different work durations, with respect to a target work condition, utilizing the electric motor output torque corresponding to each work duration, combined with the current vibration load and mesh load of a bearing, the equivalent average load under the target work condition is calculated; thus, the service life of the bearing is determined on the basis of the equivalent average load. As such, the calculated service life of the bearing is of increased accuracy, thereby allowing an improved determination to be made on an operating state of a transmission system, thus increasing the operational reliability of the transmission system.

Abstract: A planning method of EV fast charging stations on the expressway, comprising the following steps: Step 1: forecasting the spatial and temporal distribution of EV charging load, that is to determine the time and location of each EV needing charging on the expressway; Step 2: based on the forecast result achieved by Step 1, determining the locations of the fast charging stations on the expressway by the nearest neighbor clustering algorithm; Step 3: according to the spatial and temporal forecast result of the EV charging load and the locations of the fast charging stations, determining the number c of the chargers in each fast charging station by queuing theory. Due to battery characteristics, traditional gas stations do not completely match the fast charging stations.

Abstract: A planning method of EV fast charging stations on the expressway, comprising the following steps: Step 1: forecasting the spatial and temporal distribution of EV charging load, that is to determine the time and location of each EV needing charging on the expressway; Step 2: based on the forecast result achieved by Step 1, determining the locations of the fast charging stations on the expressway by the nearest neighbor clustering algorithm; Step 3: according to the spatial and temporal forecast result of the EV charging load and the locations of the fast charging stations, determining the number c of the chargers in each fast charging station by queuing theory. Due to battery characteristics, traditional gas stations do not completely match the fast charging stations.

Abstract: A pickup apparatus includes a digital signal processor, at least one microphone, multiple amplifiers, and multiple analog-to-digital converters (ADCs), where each microphone is configured to receive an audio signal, and send the audio signal to an amplifier corresponding to the microphone, each amplifier is configured to amplify the received audio signal, and send the amplified audio signal to an analog-to-digital converter corresponding to the amplifier, each analog-to-digital converter is configured to convert the received audio signal into a digital signal, and send the digital signal to the digital signal processor, and the digital signal processor is configured to receive multiple digital signals sent by the multiple analog-to-digital converters, determine an unclipped digital signal from the multiple received digital signals, and determine an output signal according to a gain value corresponding to the unclipped digital signal.

Abstract: A pickup apparatus includes a digital signal processor, at least one microphone, multiple amplifiers, and multiple analog-to-digital converters (ADCs), where each microphone is configured to receive an audio signal, and send the audio signal to an amplifier corresponding to the microphone, each amplifier is configured to amplify the received audio signal, and send the amplified audio signal to an analog-to-digital converter corresponding to the amplifier, each analog-to-digital converter is configured to convert the received audio signal into a digital signal, and send the digital signal to the digital signal processor, and the digital signal processor is configured to receive multiple digital signals sent by the multiple analog-to-digital converters, determine an unclipped digital signal from the multiple received digital signals, and determine an output signal according to a gain value corresponding to the unclipped digital signal.

Abstract: A manufacturing method of an anode foil for an aluminum electrolytic capacitor is provided, which comprises a first step of forming a porous oxide film, i.e. subjecting an etched foil having etched holes thereon to an anodic oxidation process to form a porous oxide film on both the outer surface of the etched foil and the inner surface of etched holes, and a second step of forming a dense oxide film, i.e. converting the porous oxide film into the dense oxide film. The method can be used to manufacture an anode foil for various voltage ranges, e.g. an ultra-high voltage anode foil whose voltage is more than 800 vf, and the method can increase specific capacity, reduce power consumption, simplify the process, and increase production efficiency.

Abstract: A manufacturing method of an anode foil for an aluminum electrolytic capacitor is provided, which comprises a first step of forming a porous oxide film, i.e. subjecting an etched foil having etched holes thereon to an anodic oxidation process to form a porous oxide film on both the outer surface of the etched foil and the inner surface of etched holes, and a second step of forming a dense oxide film, i.e. converting the porous oxide film into the dense oxide film. The method can be used to manufacture an anode foil for various voltage ranges, e.g. an ultra-high voltage anode foil whose voltage is more than 800 vf, and the method can increase specific capacity, reduce power consumption, simplify the process, and increase production efficiency.