Abstract: The invention discloses a monoclonal antibody against human GPR48 and application of the same. In the antibody provided by the present invention, the amino acid sequences of LCDR1, LCDR2, and LCDR3 in the light chain variable region are shown in positions 27-32, 50-52, and 89-97 of SEQ ID No.1 sequentially, and the amino acid sequences of HCDR1, HCDR2 and HCDR3 in the heavy chain variable region are shown in positions 26-33, 51-60 and 99-108 of SEQ ID No.2 sequentially. The antibody has strong specificity and sensitivity to GPR48 protein, and can be applied to immunological experimental techniques such as western blotting, immunofluorescence and immunohistochemistry; it can be applied to flow cytometry experimental techniques such as flow cyometry analysis and flow cytometry sorting; it can be applied to the scientific research of signaling pathways such as subcellular localization and protein interaction.

Abstract: A continuous welding method and device for a hybrid welding, a welded finished product and a train body. The method comprises: performing hybrid welding on a groove of a welding piece by coupling a laser and a variable polarity arc; wherein the defocusing distance of the laser is not less than a Rayleigh length of the laser. According to the method of the present disclosure, the power density of the laser on the surface of the welding piece is effectively reduced and the height-width-ratio of the weld seam is decreased, the diameter of the welding melted pore is increased, the voids caused by the collapse of the small pore in the welding melted pore can be effectively reduced, thereby solving the problem that the weld porosity is difficult to escape in the prior art, and reducing the generation of the pores, effectively improving welding stability and reliability, and improving the mechanical properties of the weld seams.

Abstract: Disclosed are a pyrimidine small-molecule compound and an application thereof. The pyrimidine small-molecule compound has efficient inhibitory activity towards PDGFR? and PDGFR? kinases, but has relatively weak inhibitory activity towards other kinases. Therefore, the compound has very high selectivity and is capable of being used as a potential drug for resisting tumors or relieving ophthalmic diseases such as wet age-related macular degeneration or uveitis.

Abstract: Disclosed are a pyrimidine small-molecule compound and an application thereof. The pyrimidine small-molecule compound has efficient inhibitory activity towards PDGFR? and PDGFR? kinases, but has relatively weak inhibitory activity towards other kinases. Therefore, the compound has very high selectivity and is capable of being used as a potential drug for resisting tumors or relieving ophthalmic diseases such as wet age-related macular degeneration or uveitis.

Abstract: The present disclosure discloses a genetically engineered strain, belonging to the technical field of bioengineering. L-amino acid oxidase genes, ?-keto acid decarboxylase genes, alcohol dehydrogenase genes, and enzyme genes capable of reducing NAD(P) to NAD(P)H are introduced into the genetically engineered strain of the present disclosure. The present disclosure further discloses a construction method and application of a recombinant Escherichia coli genetically engineered strain. When being applied to the biosynthesis of phenylethanoids, the method of the present disclosure has the characteristics of simple operation, low cost, and high synthesis efficiency and optical purity of the product, and has good industrialization prospects.

Abstract: Provided are a tripeptide compound, a preparation method therefor, and an application thereof. The structure of the related compound is represented by formula (I). The provided compound has angiotensin converting enzyme inhibiting bioactivity, and the compound and a pharmaceutical composition thereof play a role in preventing and treating hypertension and other cardiocerebral vascular system diseases.

Abstract: The disclosure discloses a strain and method for producing rosmarinic acid, and belongs to the technical field of bioengineering. The disclosure constructs a recombinant cell or a combination of recombinant cells expressing 4-coumarate: CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I (PPK2-I) and polyphosphate kinase 2-II (PPK2-II), and utilizes the recombinant cell or the combination of recombinant cells to catalyze Danshensu and caffeic acid for synthesizing rosmarinic acid. The disclosure has good industrial application prospects.

Abstract: An interactive application processing method is described. A selection of an online interactive activity by a user is received in an interactive application. The online interactive activity is associated with an offline interactive activity. According to a past performance result, a determination is made, by circuitry of an interactive application apparatus, as to whether the user qualifies to participate in the offline interactive activity. When the user is determined to qualify to participate in the offline interactive activity, identity information of the user is acquired. A certificate is generated based on the identity information for allowing the user to participate in the offline interactive activity.

Abstract: Use of isopropyl ?-(3,4-dihydroxyphenyl)-?-hydroxypropionate (IDHP) in the preparation of a drug or a health product for prevention and treatment of coronary atherosclerosis diseases. The IDHP can also be used to inhibit platelet aggregation, to promote angiogenesis, and to prepare a drug and a health product for prevention and treatment of senile vascular dementia.

Abstract: The present disclosure discloses an engineering strain and application thereof in joint production of Danshensu and alanine, and belongs to the technical field of bioengineering. The present disclosure constructs a three-enzyme co-expression genetic engineering strain, and realizes joint production of Danshensu and alanine. Further, the transport of a substrate is promoted and decomposition of products is reduced by knocking out or enhancing expression of related genes on E. coli genome. The genetic engineering strain provided by the present disclosure can produce optically pure D-danshensu and L-danshensu, and jointly produce pyruvic acid. The production process is simple, raw materials are easily available, impurities are fewer, and a good industrial application prospect is achieved.

Abstract: The present disclosure discloses a production method of Danshensu, belonging to the technical field of bioengineering. The present disclosure constructs a novel genetic engineering strain co-expressed by three enzymes, which can be applied to the production of optically pure 3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid. All of the (D/L)-?-hydroxycarboxylic acid dehydrogenase selected by the present disclosure have the characteristics of poor substrate specificity and strong optical specificity, and can produce optically pure D-danshensu and L-danshensu. Further, the production efficiency of the recombinant strain is improved by knocking out or enhancing the expression of a related gene on the E. coli genome to promote substrate transport and reduce product decomposition.

Abstract: Embodiments of this application provide a near-field wireless communication service processing method performed at a first computing device associated with a social networking application. While running a social networking application, the first computing device detects a near-field wireless communication signal broadcasted by a second computing device associated with a service provider account registered with the social networking application.

Abstract: Use of isopropyl ?-(3,4-dihydroxyphenyl)-?-hydroxypropionate (IDHP) in the preparation of a drug or a health product for prevention and treatment of coronary atherosclerosis diseases. The IDHP can also be used to inhibit platelet aggregation, to promote angiogenesis, and to prepare a drug and a health product for prevention and treatment of senile vascular dementia.

Abstract: The present disclosure discloses an engineering strain and application thereof in joint production of Danshensu and alanine, and belongs to the technical field of bioengineering. The present disclosure constructs a three-enzyme co-expression genetic engineering strain, and realizes joint production of Danshensu and alanine. Further, the transport of a substrate is promoted and decomposition of products is reduced by knocking out or enhancing expression of related genes on E. coli genome. The genetic engineering strain provided by the present disclosure can produce optically pure D-danshensu and L-danshensu, and jointly produce pyruvic acid. The production process is simple, raw materials are easily available, impurities are fewer, and a good industrial application prospect is achieved.

Abstract: The present disclosure discloses a production method of Danshensu, belonging to the technical field of bioengineering. The present disclosure constructs a novel genetic engineering strain co-expressed by three enzymes, which can be applied to the production of optically pure 3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid. All of the (D/L)-?-hydroxycarboxylic acid dehydrogenase selected by the present disclosure have the characteristics of poor substrate specificity and strong optical specificity, and can produce optically pure D-danshensu and L-danshensu. Further, the production efficiency of the recombinant strain is improved by knocking out or enhancing the expression of a related gene on the E. coli genome to promote substrate transport and reduce product decomposition.

Abstract: Embodiments of this application provide a near-field wireless communication service processing method performed at a first computing device associated with a social networking application. While running a social networking application, the first computing device detects a near-field wireless communication signal broadcasted by a second computing device associated with a service provider account registered with the social networking application.

Abstract: Embodiments of this application provide a near-field wireless communication service processing method performed at a first computing device. While running a social networking application, the first computing device listens to a near-field wireless communication signal broadcasted by a second computing device. After detecting the near-field wireless communication signal broadcasted by the second computing device, first computing device processes the near-field wireless communication signal to obtain identification information associated with the second computing device. The first computing device sends the identification information associated with the second computing device to a remote server supporting the social networking application and obtains a preset service page corresponding to the identification information associated with the second computing device from the server, and displays the preset service page within the social networking application on the first computing device.

Abstract: An interactive application processing method is described. A selection of an online interactive activity by a user is received in an interactive application. The online interactive activity is associated with an offline interactive activity. According to a past performance result, a determination is made, by circuitry of an interactive application apparatus, as to whether the user qualifies to participate in the offline interactive activity. When the user is determined to qualify to participate in the offline interactive activity, identity information of the user is acquired. A certificate is generated based on the identity information for allowing the user to participate in the offline interactive activity.