Abstract: RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.

Abstract: The present disclosure relates to systems, methods and compositions provided herein that include a compound protease. The compound protease can contain a protease domain, a cut site for another enzyme and an association domain. In some embodiments, the compound protease is part of a protein circuit.

Abstract: Disclosed herein include methods, compositions, and systems suitable for use in delivering a polynucleotide to a target cell of a subject in need thereof. In some embodiments, a viral vector comprises a polynucleotide encoding nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L), and one or more transgenes. The viral vector can comprise one or more of a conditionally stable fusion protein, a protease fusion protein, a degron fusion protein, and/or a glycoprotein derived of another species than the viral vector polynucleotide to enable control of viral vector transduction and/or replication.

Abstract: Disclosed is a sulfate corrosion-resistant concrete, a method for optimizing proportion and application thereof. The concrete is formed by mixing and stirring base stocks, aggregates, admixtures, external additives and water. The base stock of the concrete is 17.4-17.5 parts of Portland cement; the aggregates include 38.9 parts of basalt with aggregate size of 5-10 mm and 33.1-33.2 parts of basalt medium sand; the admixtures are 1.9-1.95 parts of silica fume or fly ash, and further including 0.23-0.24 part of polycarboxylate water reducer and 1.34-1.35 part of sulfate corrosion-resistant liquid preservative. Optimized proportion method: according to the corrosion characteristics of sulfate and corrosion environment parameters, determine the composition and proportion of basic samples and comparison samples, make and cure sample components, test the deep components of the samples, and obtain the optimal composition and proportion according to the test results.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in detecting the activation level of a signal transducer. In some embodiments, there are provided synthetic protein circuits wherein recruitment of synthetic protein circuit components to an association location upon activation of a signal transducer generates an active effector protein. The effector protein can be configured to carry out a variety of functions when in an active state, such as, for example, inducing cell death. Methods of treating a disease or disorder characterized by aberrant signaling are provided in some embodiments.

Abstract: Disclosed herein include methods, compositions, and systems suitable for use in delivering a polynucleotide to a target cell of a subject in need thereof. In some embodiments, a viral vector comprises a polynucleotide encoding nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L), and one or more transgenes. The viral vector can comprise one or more of a conditionally stable fusion protein, a protease fusion protein, a degron fusion protein, and/or a glycoprotein derived of another species than the viral vector polynucleotide to enable control of viral vector transduction and/or replication.

Abstract: To program intercellular communication for biomedicine, it is crucial to regulate the secretion and surface display of signaling proteins. If such regulations are at the protein level, there are additional advantages, including compact delivery and direct interactions with endogenous signalling pathways. A modular, generalizable design is provided called Retained Endoplasmic Cleavable Secretion (RELEASE), with engineered proteins retained in the endoplasmic reticulum and displayed/secreted in response to specific proteases. The design allows functional regulation of multiple synthetic and natural proteins by synthetic protease circuits to realize diverse signal processing capabilities, including logic operation and threshold tuning. By linking RELEASE to additional novel sensing and processing circuits, one would be able to achieve elevated protein secretion in response to “undruggable” oncogene KRAS mutants.

Abstract: Disclosed is a sulfate corrosion-resistant concrete, a method for optimizing proportion and application thereof. The concrete is formed by mixing and stirring base stocks, aggregates, admixtures, external additives and water. The base stock of the concrete is 17.4-17.5 parts of Portland cement; the aggregates include 38.9 parts of basalt with aggregate size of 5-10 mm and 33.1-33.2 parts of basalt medium sand; the admixtures are 1.9-1.95 parts of silica fume or fly ash, and further including 0.23-0.24 part of polycarboxylate water reducer and 1.34-1.35 part of sulfate corrosion-resistant liquid preservative. Optimized proportion method: according to the corrosion characteristics of sulfate and corrosion environment parameters, determine the composition and proportion of basic samples and comparison samples, make and cure sample components, test the deep components of the samples, and obtain the optimal composition and proportion according to the test results.

Abstract: Provided is a method for analyzing the expansive stress and expansive strain of tunnel surrounding rock, including: (1) determining the surrounding rock state of the tunnel surrounding rock before expansion and the surrounding rock state after expansion; (2) according to the determined state of the surrounding rock, determining the expansive stress and expansive strain of the tunnel surrounding rock before expansion, and determining the expansive stress and expansive strain of the tunnel surrounding rock after expansion; (3) according to the surrounding rock state of the tunnel surrounding rock after expansion, determining the expansive deformation radius of the tunnel surrounding rock, so as to determine the expansive deformation displacement of the tunnel surrounding rock.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in detecting the activation level of a signal transducer. In some embodiments, there are provided synthetic protein circuits wherein recruitment of synthetic protein circuit components to an association location upon activation of a signal transducer generates an active effector protein. The effector protein can be configured to carry out a variety of functions when in an active state, such as, for example, inducing cell death. Methods of treating a disease or disorder characterized by aberrant signaling are provided in some embodiments.

Abstract: The invention discloses a method for analyzing the expansive stress and expansive strain of tunnel surrounding rock, including: (1) determining the surrounding rock state of the tunnel surrounding rock before expansion and the surrounding rock state after expansion; (2) according to the determined state of the surrounding rock, determining the expansive stress and expansive strain of the tunnel surrounding rock before expansion, and determining the expansive stress and expansive strain of the tunnel surrounding rock after expansion; (3) according to the surrounding rock state of the tunnel surrounding rock after expansion, the expansive deformation radius of the tunnel surrounding rock is determined, so as to determine the expansive deformation displacement of the tunnel surrounding rock.

Abstract: The invention discloses a method for analyzing the constitutive relationship of confined rock expansion, including: (1) determining parameters that affect rock expansive properties, wherein the parameters comprise category, density, and water content; (2) establishing an expression of the relationship between the parameters and rock expansive capacity; (3) establishing an expression of the relationship among the rock expansive capacity, rock expansive stress and rock expansive strain; and (4) determining an expression of the relationship among the parameters, the rock expansive stress and the rock expansive strain according to the rock expansive capacity. The present invention introduces parameters comprising category, density, and water content that affect the rock expansive properties into the analysis of rock expansive stress and rock expansive strain, which can truly reflect the variation of rock expansive stress and rock expansive strain, and can achieve quantized calculation of the rock expansive state.

Abstract: Disclosed herein include methods, compositions, and systems suitable for use in delivering a polynucleotide to a target cell of a subject in need thereof. In some embodiments, a viral vector comprises a polynucleotide encoding nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L), and one or more transgenes. The viral vector can comprise one or more of a conditionally stable fusion protein, a protease fusion protein, a degron fusion protein, and/or a glycoprotein derived of another species than the viral vector polynucleotide to enable control of viral vector transduction and/or replication.

Abstract: Some embodiments of the systems, methods and compositions provided herein relate to a compound protease. In some embodiments, the compound protease includes a protease domain and a cut site for another enzyme. In some embodiments, the compound protease includes an association domain. In some embodiments, the compound protease is part of a protein circuit.

Abstract: Some embodiments of the systems, methods and compositions provided herein relate to a compound protease. In some embodiments, the compound protease includes a protease domain and a cut site for another enzyme. In some embodiments, the compound protease includes an association domain. In some embodiments, the compound protease is part of a protein circuit.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in detecting the activation level of a signal transducer. In some embodiments, there are provided synthetic protein circuits wherein recruitment of synthetic protein circuit components to an association location upon activation of a signal transducer generates an active effector protein. The effector protein can be configured to carry out a variety of functions when in an active state, such as, for example, inducing cell death. Methods of treating a disease or disorder characterized by aberrant signaling are provided in some embodiments.

Abstract: Disclosed herein include methods, compositions, and systems suitable for use in delivering a polynucleotide to a target cell of a subject in need thereof. In some embodiments, a viral vector comprises a polynucleotide encoding nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L), and one or more transgenes. The viral vector can comprise one or more of a conditionally stable fusion protein, a protease fusion protein, a degron fusion protein, and/or a glycoprotein derived of another species than the viral vector polynucleotide to enable control of viral vector transduction and/or replication.

Abstract: Some embodiments of the systems, methods and compositions provided herein relate to a compound protease. In some embodiments, the compound protease includes a protease domain and a cut site for another enzyme. In some embodiments, the compound protease includes an association domain. In some embodiments, the compound protease is part of a protein circuit.