Abstract: Methods and compositions are provided for treating an individual in need (e.g., one who has chronic pain). Such methods can include a step of administering a therapy that reduces CamKv activity in opioid receptor mu 1 (OPRM1) expressing neurons of the individual's rostral ventromedial medulla (RVM). In some cases, the therapy (e.g., deep brain stimulation of zona incerta neurons) increases inhibitory input into the individual's RVM. In some cases, the therapy is an agent (e.g., an RNAi agent) that reduces expression or activity of CamKv in the individual's RVM. In some cases, the agent includes a retrograde-enhanced recombinant AAV particle, e.g., one that can be used to deliver an RNAi agent such as an shRNA that targets CamKv. Also provide are retrograde-enhanced clade E variant AAV capsid proteins, AVV particles that include such capsid proteins, methods of making such AAV particles, and methods of expressing a transgene using such AAVs.

Abstract: A reinforced waterproof outdoor lamp, including a first electric conductor, a second electric conductor, a light emitting source, a waterproof plug, a lamp holder, a lamp housing and waterproof glue. The waterproof plug is formed on the first electric conductor and the second electric conductor by means of a mold through plastic filling or injection molding.

Abstract: The present disclosure relates to systems and methods for operating an online on-demand service platform. The systems may perform the methods to: receive confirmation that a service provider has accepted a service request from a service requester; acquire feature information associated with the service provider; acquire surrounding information associated with the service provider; generate identification information of the service provider based on the feature information or the surrounding information for the service requester to identify the service provider; and transmit the identification information of the service provider to a requester terminal associated with the service requester.

Abstract: The present disclosure relates to systems and methods for operating an online on-demand service platform. The systems may perform the methods to: receive confirmation that a service provider has accepted a service request from a service requester; acquire feature information associated with the service provider; acquire surrounding information associated with the service provider; generate identification information of the service provider based on the feature information or the surrounding information for the service requester to identify the service provider; and transmit the identification information of the service provider to a requester terminal associated with the service requester.